Comprehensive analysis of NAC transcription factors in Scutellaria baicalensis and their response to exogenous ABA and GA3

He, Hongbin; Li, Qiuyue; Fang, Liang Xing; Ye, Wen; Xu, Feng; Yan, Yan; Mao, Renjun

doi:10.1016/j.ijbiomac.2023.125290

Cited by 8 publications

(7 citation statements)

References 53 publications

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“…Accordingly, EPS not only stimulates the expression of ABA and GA key enzyme genes (GA2ox and CYP707A) in S. baicalensis calli but also promotes the expression of MYB3, MYB8, and MYB13. Based on our experimental results and relevant literature (Bang et al, 2008;Yuan et al, 2013Yuan et al, , 2015Qian et al, 2020;Zhou et al, 2022;He et al, 2023), EPS treatment induces flavonoid synthesis in S. baicalensis calli by regulating the expression of phytohormones, TFs, and enzymes. That is, EPS might stimulate the expression of genes encoding MYB3, MYB8, MYB13, GA2ox, and CYP707A, leading to significantly higher expression of CHS, CHI, FNS, and F6H and ultimately promoting the synthesis of flavonoids.…”

Section: Discussionmentioning

confidence: 72%

“…ABA and GA can promote flavonoid synthesis in S. baicalensis ( Yuan et al., 2013 ; He et al., 2023 ). Our RNA-seq results revealed significant up-regulation of GA2ox and CYP707A , which are involved in GA and ABA synthesis, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…The formation and accumulation of plant secondary metabolites are regulated by a complex interplay of plant hormones and transcription factors (TFs). Plant hormones, such as gibberellic acid (GA) and abscisic acid (ABA), reportedly promote flavonoid production in S. baicalensis ( Yuan et al., 2013 ; He et al., 2023 ). Meanwhile, TFs, such as the NAC and MYB families, are key regulators of flavonoid synthesis ( Yuan et al., 2013 , 2015 ; Qian et al., 2020 ; Fang et al., 2023 , 2023 ; He et al., 2023 ).…”

Section: Introductionmentioning

confidence: 99%

“…Plant hormones, such as gibberellic acid (GA) and abscisic acid (ABA), reportedly promote flavonoid production in S. baicalensis ( Yuan et al., 2013 ; He et al., 2023 ). Meanwhile, TFs, such as the NAC and MYB families, are key regulators of flavonoid synthesis ( Yuan et al., 2013 , 2015 ; Qian et al., 2020 ; Fang et al., 2023 , 2023 ; He et al., 2023 ). The crosstalk between plant hormones and TFs adds another layer of regulation to secondary metabolite formation and accumulation ( Bang et al., 2008 ; Yuan et al., 2013 ; Zhou et al., 2022 ).…”

Section: Introductionmentioning

confidence: 99%

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Effects of elicitors from culture filtrate of Fusarium solani CL105 on flavonoid production of Scutellaria baicalensis calli

Cui,

Zhang,

Sun

et al. 2024

Front. Plant Sci.

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IntroductionEndophytic fungi can promote secondary metabolite accumulation in medicinal plants. Previously, we observed that the culture filtrate of Fusarium solani CL105 promoted flavonoid production in Scutellaria baicalensis calli. However, the active ingredients and mechanisms associated with this secondary metabolite accumulation remain unclear.MethodsThis study evaluates the effects of different elicitors from the culture filtrate of F. solani CL105 namely, exopolysaccharide (EPS), exoprotein (EP), and other parts (OP), on the flavonoid production in S. baicalensis calli by HPLC. Subsequently, the underlying mechanism of EPS induced flavonoid production in S. baicalensis calli was revealed by transcriptomics and RT-PCR.Results and discussionThe results indicated a significant increase in flavonoid production in S. baicalensis calli following treatment with EPS. Baicalin (1.40 fold), wogonoside (1.91 fold), and wogonin (2.76 fold) were most significantly up-regulated compared with the control. Transcriptome analysis further revealed up-regulation of key enzyme genes (CHS, CHI, FNS, and F6H) involved in flavonoid synthesis after 5 days of EPS treatment. Moreover, the expression of GA2ox and CYP707A—genes involved in gibberellin acid (GA) and abscisic acid biosynthesis (ABA), respectively—were significantly up-regulated. The expression levels of certain transcription factors, including MYB3, MYB8, and MYB13, were also significantly higher than in controls. Our results indicated that EPS was a main active elicitor involved in promoting flavonoid production in S. baicalensis calli. We postulated that EPS might stimulate the expression of MYB3, MYB8, MYB13, GA2ox, and CYP707A, leading to markedly upregulated CHS, CHI, FNS, and F6H expression levels, ultimately promoting flavonoid synthesis. This study provides a novel avenue for large-scale in vitro production of flavonoids in S. baicalensis.

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Section: Discussionmentioning

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of elicitors from culture filtrate of Fusarium solani CL105 on flavonoid production of Scutellaria baicalensis calli

Cui,

Zhang,

Sun

et al. 2024

Front. Plant Sci.

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“…In recent years, NACs have been elaborately studied in medicinal plants. Twelve SbNACs are involved in regulating the synthesis of flavonoids in the flowers of Scutellaria baicalensis [27]. PgNAC41-2 positively regulates the biosynthesis of saponins in Panax ginseng [28] and the NAC family acts as a regulatory effector in the synthesis of a variety of metabolites under salt stress in Isatis indigotica [29].…”

Section: Introductionmentioning

confidence: 99%

Comprehensive Analysis of NAC Transcription Factors Reveals Their Evolution in Malvales and Functional Characterization of AsNAC019 and AsNAC098 in Aquilaria sinensis

Yang,

Mei,

Wang

et al. 2023

IJMS

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NAC is a class of plant-specific transcription factors that are widely involved in the growth, development and (a)biotic stress response of plants. However, their molecular evolution has not been extensively studied in Malvales, especially in Aquilaria sinensis, a commercial and horticultural crop that produces an aromatic resin named agarwood. In this study, 1502 members of the NAC gene family were identified from the genomes of nine species from Malvales and three model plants. The macroevolutionary analysis revealed that whole genome duplication (WGD) and dispersed duplication (DSD) have shaped the current architectural structure of NAC gene families in Malvales plants. Then, 111 NAC genes were systemically characterized in A. sinensis. The phylogenetic analysis suggests that NAC genes in A. sinensis can be classified into 16 known clusters and four new subfamilies, with each subfamily presenting similar gene structures and conserved motifs. RNA-seq analysis showed that AsNACs presents a broad transcriptional response to the agarwood inducer. The expression patterns of 15 AsNACs in A. sinensis after injury treatment indicated that AsNAC019 and AsNAC098 were positively correlated with the expression patterns of four polyketide synthase (PKS) genes. Additionally, AsNAC019 and AsNAC098 were also found to bind with the AsPKS07 promoter and activate its transcription. This comprehensive analysis provides valuable insights into the molecular evolution of the NAC gene family in Malvales plants and highlights the potential mechanisms of AsNACs for regulating secondary metabolite biosynthesis in A. sinensis, especially for the biosynthesis of 2-(2-phenyl) chromones in agarwood.

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Sulfur redirects carbon metabolism to optimize nitrogen utilization and promote andrographolide biosynthesis in Andrographis paniculata seedlings

Jian,

Huang,

Wan

et al. 2024

Current Plant Biology

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Comprehensive analysis of NAC transcription factors in Scutellaria baicalensis and their response to exogenous ABA and GA3

Cited by 8 publications

References 53 publications

Effects of elicitors from culture filtrate of Fusarium solani CL105 on flavonoid production of Scutellaria baicalensis calli

Effects of elicitors from culture filtrate of Fusarium solani CL105 on flavonoid production of Scutellaria baicalensis calli

Comprehensive Analysis of NAC Transcription Factors Reveals Their Evolution in Malvales and Functional Characterization of AsNAC019 and AsNAC098 in Aquilaria sinensis

Sulfur redirects carbon metabolism to optimize nitrogen utilization and promote andrographolide biosynthesis in Andrographis paniculata seedlings

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