Comprehensive Analysis of Potential miRNA-Target mRNA-Immunocyte Subtype Network in Cerebral Infarction

Qi, Xiuyan; Lin, Huiqian; Hou, Yongge; Su, Xuantao; Gao, Yanfang

doi:10.1159/000518893

Cited by 5 publications

(4 citation statements)

References 65 publications

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“…miR-330-3p exhibits therapeutic significance in neuropathic pain [25]. Consistent with previous findings [16], our study revealed an upregulation of miR-330-3p in patients with ACI compared with that in healthy controls. The diagnosis significance of miR-330-3p for patients with ACI was assessed using an ROC curve, which confirmed its high diagnostic value for ACI.…”

Section: Discussionsupporting

confidence: 92%

“…Moreover, miR-330 levels could impact glial fibrillary acidic protein during neural stem cell differentiation, and aberrantly high expression of miR-330 due to PTEN-induced kinase 1 gene deficiency could result in abnormal astrocyte development [15]. miR-330-3p has been identified as a dysregulated miRNA in cerebral infarction using bioinformatic analysis [16]. However, its significance in ACI diagnosis and prognosis has not been illustrated.…”

Section: Introductionmentioning

confidence: 99%

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Clinical Significance of MicroRNA-330-3p in Plasma Level for Acute Cerebral Infarction

Jian,

Wang,

Wang

et al. 2023

Cerebrovasc Dis

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Introduction: The present study was to investigate the clinical role of miR-330-3p in acute cerebral infarction (ACI), including its diagnostic and prognostic potential. Preliminary exploration of its target genes was archived by bioinformatics analysis. Methods: miR-330-3p in plasma of the patients with ACI and controls were quantified by real-time quantitative PCR. The 1-month prognosis of the ACI patients was evaluated by the Glasgow Outcome Scale (GOS). The correlation between the plasma levels of miR-330-3p and the GOS scores was tested by Pearson correlation analysis. The receiver operating characteristic (ROC) curves were established based on the expression level of miR-330-3p in different groups. The miR-330-3p-targeting genes were analyzed using Venn diagram, protein-protein interaction network, and Gene Ontology enrichment analysis. Results: miR-330-3p was significantly increased in the plasma of ACI patients compared with that in healthy controls, and ROC curve revealed its diagnostic value for ACI. miR-330-3p was significantly increased in the plasma of patients with poor 1-month prognosis compared with those with good 1-month prognosis. miR-330-3p expression was negatively correlated with GOS score, suggesting its potential to predict the 1-month prognosis for ACI. One-year survival analysis revealed surviving patients had lower levels of miR-330-3p than the deceased. miR-330-3p was proven to predict the death of patients with ACI. The miR-330-3p-targeting genes were associated with synapse-related Gene Ontology terms. Conclusion: miR-330-3p was upregulated in the plasma of patients with ACI, making it a promising diagnostic and prognostic marker for patients with ACI. miR-330-3p could facilitate synaptic plasticity following cerebral infarction.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Clinical Significance of MicroRNA-330-3p in Plasma Level for Acute Cerebral Infarction

Jian,

Wang,

Wang

et al. 2023

Cerebrovasc Dis

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“…The three miRNAs identified were hsa-miR-548ag, hsa-miR-329-5p, and hsa-miR-625-3p. The expression of hsa-miR-625-3p was correlated with cholesterol levels ( 46 ) and hsa-miR-625-3p was reported to be interrelated with cerebral infarction ( 47 ). Given these findings, the identified transcription factors and miRNAs were essential to the ferroptosis dysfunction in IS.…”

Section: Discussionmentioning

confidence: 99%

Ferroptosis-related differentially expressed genes serve as new biomarkers in ischemic stroke and identification of therapeutic drugs

Zhang

Yao

et al. 2022

Front. Nutr.

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BackgroundIron is an essential nutrient element, and iron metabolism is related to many diseases. Ferroptosis is an iron-dependent form of regulated cell death associated with ischemic stroke (IS). Hence, this study intended to discover and validate the possible ferroptosis-related genes involved in IS.Materials and methodsGSE16561, GSE37587, and GSE58294 were retrieved from the GEO database. Using R software, we identified ferroptosis-related differentially expressed genes (DEGs) in IS. Protein-protein interactions (PPIs) and enrichment analyses were conducted. The ROC curve was plotted to explore the diagnostic significance of those identified genes. The consistent clustering method was used to classify the IS samples. The level of immune cell infiltration of different subtypes was evaluated by ssGSEA and CIBERSORT algorithm. Validation was conducted in the test sets GSE37587 and GSE58294.ResultsTwenty-one ferroptosis-related DEGs were detected in IS vs. the normal controls. Enrichment analysis shows that the 21 DEGs are involved in monocarboxylic acid metabolism, iron ion response, and ferroptosis. Moreover, their expression levels were pertinent to the age and gender of IS patients. The ROC analysis demonstrated remarkable diagnostic values of LAMP2, TSC22D3, SLC38A1, and RPL8 for IS. Transcription factors and targeting miRNAs of the 21 DEGs were determined. Vandetanib, FERRIC CITRATE, etc., were confirmed as potential therapeutic drugs for IS. Using 11 hub genes, IS patients were categorized into C1 and C2 subtypes. The two subtypes significantly differed between immune cell infiltration, checkpoints, and HLA genes. The 272 DEGs were identified from two subtypes and their biological functions were explored. Verification was performed in the GSE37587 and GSE58294 datasets.ConclusionOur findings indicate that ferroptosis plays a critical role in the diversity and complexity of the IS immune microenvironment.

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“…MiR-671-5p can also act as a target to mediate immune regulation, affect cytokine production, and changes in antigen-related marker levels ( 32 ). Meanwhile, miR-671-3p exists in the miRNA-mRNA-immune cell regulatory network ( 33 ). It can be a biomarker for predicting and diagnosing grades II-IV acute graft-versus-host disease ( 34 ).…”

Section: Mir-671 In Bone-related Inflammatory Diseasesmentioning

confidence: 99%

Research progress on the role and mechanism of miR-671 in bone metabolism and bone-related diseases

Wang

Hu²,

Song³

et al. 2023

Front. Oncol.

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Bone metabolism consists of bone formation and resorption and maintains a dynamic balance in vivo. When bone homeostasis is broken, it can manifest as osteoarthritis (OA), rheumatoid arthritis (RA), osteosarcoma (OS), etc. MiR-671, an important class of non-coding nucleotide sequences in vivo, is regulated by lncRNA and regulates bone metabolism balance by regulating downstream target proteins and activating various signaling pathways. Based on the structure and primary function of miR-671, this paper summarizes the effect and mechanism of miR-671 in bone-related inflammation and cancer diseases, and prospects the application possibility of miR-671, providing reference information for targeted therapy of bone-related disorders.

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Comprehensive Analysis of Potential miRNA-Target mRNA-Immunocyte Subtype Network in Cerebral Infarction

Cited by 5 publications

References 65 publications

Clinical Significance of MicroRNA-330-3p in Plasma Level for Acute Cerebral Infarction

Clinical Significance of MicroRNA-330-3p in Plasma Level for Acute Cerebral Infarction

Ferroptosis-related differentially expressed genes serve as new biomarkers in ischemic stroke and identification of therapeutic drugs

Research progress on the role and mechanism of miR-671 in bone metabolism and bone-related diseases

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