Comprehensive Analysis of the COBRA-Like (COBL) Gene Family in Gossypium Identifies Two COBLs Potentially Associated with Fiber Quality

Niu, Erli; Shang, Xiaoguang; Cheng, Chaoze; Bao, Jiang-hao; Zeng, Y. Q.; Cai, Caiping; Du, Xiongming; Guo, Wangzhen

doi:10.1371/journal.pone.0145725

Cited by 53 publications

(40 citation statements)

References 63 publications

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“…A subset of COBRAs appear to be specialized for function in secondary cell walls, which typically consist of 40%-50% cellulose and are thickened to provide rigidity (Zhong and Ye 2015). COBRAs are therefore implicated in regulating wood and fiber quality (Gritsch et al 2015;Niu et al 2015).…”

Section: Gaps and Cell Wall Biosynthesis And Maintenancementioning

confidence: 99%

Plant glycosylphosphatidylinositol anchored proteins at the plasma membrane‐cell wall nexus

Yeats

Bacic

Johnson

2018

JIPB

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Approximately 1% of plant proteins are predicted to be post-translationally modified with a glycosylphosphatidylinositol (GPI) anchor that tethers the polypeptide to the outer leaflet of the plasma membrane. Whereas the synthesis and structure of GPI anchors is largely conserved across eukaryotes, the repertoire of functional domains present in the GPI-anchored proteome has diverged substantially. In plants, this includes a large fraction of the GPI-anchored proteome being further modified with plant-specific arabinogalactan (AG) O-glycans. The importance of the GPI-anchored proteome to plant development is underscored by the fact that GPI biosynthetic null mutants exhibit embryo lethality. Mutations in genes encoding specific GPI-anchored proteins (GAPs) further supports their contribution to diverse biological processes, occurring at the interface of the plasma membrane and cell wall, including signaling, cell wall metabolism, cell wall polymer cross-linking, and plasmodesmatal transport. Here, we review the literature concerning plant GPI-anchored proteins, in the context of their potential to act as molecular hubs that mediate interactions between the plasma membrane and the cell wall, and their potential to transduce the signal into the protoplast and, thereby, activate signal transduction pathways.

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Section: Gaps and Cell Wall Biosynthesis And Maintenancementioning

confidence: 99%

Plant glycosylphosphatidylinositol anchored proteins at the plasma membrane‐cell wall nexus

Yeats

Bacic

Johnson

2018

JIPB

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“…For example, a gene encoding cellulose synthase (Solyc07g043390), the catalytic moiety required for the synthesis of cellulose microfibrils, was expressed at higher levels in the gpat6-a mutant. Conversely, the expression of a COBRA-like gene (SlCOBL1; Solyc02g089120), which encodes a GPIanchored protein regulating epidermal cell wall thickness and cellulose formation in tomato fruit (Cao et al, 2012;Niu et al, 2015), was lower in gpat6-a. We also detected the altered expression of genes related to pectins and hemicelluloses, the matrix polysaccharides of the primary cell wall.…”

Section: The Gpat6-a Mutant Has Abnormal Fruit Cuticle Thickness and mentioning

confidence: 99%

The glycerol-3-phosphate acyltransferase GPAT6 from tomato plays a central role in fruit cutin biosynthesis

et al. 2016

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The thick cuticle covering and embedding the epidermal cells of tomato (Solanum lycopersicum) fruit acts not only as a protective barrier against pathogens and water loss but also influences quality traits such as brightness and postharvest shelf-life. In a recent study, we screened a mutant collection of the miniature tomato cultivar Micro-Tom and isolated several glossy fruit mutants in which the abundance of cutin, the polyester component of the cuticle, was strongly reduced. We employed a newly developed mapping-by-sequencing strategy to identify the causal mutation underlying the cutin deficiency in a mutant thereafter named gpat6-a (for glycerol-3-phosphate acyltransferase6). To this end, a backcross population (BC 1 F 2 ) segregating for the glossy trait was phenotyped. Individuals displaying either a wild-type or a glossy fruit trait were then pooled into bulked populations and submitted to whole-genome sequencing prior to mutation frequency analysis. This revealed that the causal point mutation in the gpat6-a mutant introduces a charged amino acid adjacent to the active site of a GPAT6 enzyme. We further showed that this mutation completely abolished the GPAT activity of the recombinant protein. The gpat6-a mutant showed perturbed pollen formation but, unlike a gpat6 mutant of Arabidopsis (Arabidopsis thaliana), was not male sterile. The most striking phenotype was observed in the mutant fruit, where cuticle thickness, composition, and properties were altered. RNA sequencing analysis highlighted the main processes and pathways that were affected by the mutation at the transcriptional level, which included those associated with lipid, secondary metabolite, and cell wall biosynthesis.

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“…By taking the Pfam data as the query, the HD Zip members were identified using HMMER software, version 3.0 (Finn et al 2011). The phylogenetic tree of the HD-Zip gene family was built using MEGA 5.0 software (www.megasoftware.net) with the maximum likelihood method (Tamura et al 2011), using the parameters referred to Niu et al (2015).…”

Section: Identification Of the Hd-zip Gene Familymentioning

confidence: 99%

Up-regulation of a homeodomain-leucine zipper gene HD-1 contributes to trichome initiation and development in cotton

Niu

Cai

Bao

et al. 2019

Journal of Integrative Agriculture

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Plant trichomes originate from epidermal cells. In this work, we demonstrated that a homeodomain-leucine zipper gene, Gh_A06G1283 (GhHD-1A), was related to the leaf trichome trait in allotetraploid cotton and could be a candidate gene for the T 1 locus. The ortholog of GhHD-1A in the hairless accession Gossypium barbadense cv. Hai7124 was interrupted by a long terminal repeat (LTR) retrotransposon, while GhHD-1A worked well in the hairy accession G. hirsutum acc. T586. Sequence and phylogenetic analysis showed that GhHD-1A belonged to the HD-Zips IV gene family, which mainly regulated epidermis hair development in plants. Silencing of GhHD-1A and its homoeologs GhHD-1D in allotetraploid T586 and Hai7124 could significantly reduce the density of leaf hairs and affect the expression levels of other genes related to leaf trichome formation. Further analysis found that GhHD-1A mainly regulated trichome initiation on the upper epidermal hairs of leaves in cotton, while the upregulated expression of GhHD-1A in different organs/tissues also altered epidermal trichome development. This study not only helps to unravel the important roles of GhHD-1A in regulating trichome initiation in cotton, but also provides a reference for exploring the different forms of trichome development in plants.

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Comprehensive Analysis of the COBRA-Like (COBL) Gene Family in Gossypium Identifies Two COBLs Potentially Associated with Fiber Quality

Cited by 53 publications

References 63 publications

Plant glycosylphosphatidylinositol anchored proteins at the plasma membrane‐cell wall nexus

Plant glycosylphosphatidylinositol anchored proteins at the plasma membrane‐cell wall nexus

The glycerol-3-phosphate acyltransferase GPAT6 from tomato plays a central role in fruit cutin biosynthesis

Up-regulation of a homeodomain-leucine zipper gene HD-1 contributes to trichome initiation and development in cotton

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