Identification of slope subsurface strata for natural soil slopes is essential to assess the stability of potential landslides. The highly variable strata in a slope are hard to characterize by traditional boreholes at limited locations. Ground-penetrating radar (GPR) is a non-destructive method that is capable of capturing continuous subsurface information. However, the accuracy of subsurface identification using GPRs is still an open issue. This work systematically investigates the capability of the GPR technique to identify different strata via both laboratory experiments and on-site examination. Six large-scale models were constructed with various stratigraphic interfaces (i.e., sand–rock, clay–rock, clay–sand, interbedded clay, water table, and V–shaped sand–rock). The continuous interfaces of the strata in these models were obtained using a GPR, and the depths at different points of the interfaces were interpreted. The interpreted depths along the interface were compared with the measured values to quantify the interpretation accuracy. Results show that the depths of interfaces should be interpreted with the relative permittivity, back-calculated using on-site borehole information instead of empirical values. The relative errors of the depth of horizontal interfaces of different strata range within ±5%. The relative and absolute errors of the V–shaped sand–rock interface depths are in the ranges of [−9.9%, 10.5%] and [−107, 119] mm, respectively. Finally, the GPR technique was used in the field to identify the strata of a slope from Tanglang Mountain in China. The continuous profile of the subsurface strata was successfully identified with a relative error within ±5%.