Comprehensive assessment of Hypsizygus marmoreus polysaccharides through simulated digestion and gut microbiota fermentation in vitro

Kai, Ye; Fu, Chujing; Ma, Stefan; Du, Hengjun; Chen, Shiguo; Liu, Donghong; Ma, Gaoxing; Xiao, Haiyan

doi:10.1016/j.foodhyd.2023.108989

Cited by 12 publications

(1 citation statement)

References 37 publications

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“…The results confirmed that TPS MWs do not change significantly during salivary and gastric digestion (Figure 1A,B); in other words, the main TPS structure remains relatively stable throughout salivary and gastric digestion. Similar results have been noted previously for Hypsizygus marmoreus polysaccharides [40].…”

Section: Changes In Tps Mw and Monosaccharidessupporting

confidence: 91%

In Vitro Characterization of Polysaccharides from Fresh Tea Leaves in Simulated Gastrointestinal Digestion and Gut Microbiome Fermentation

Zhou,

Gao,

Sun

et al. 2024

Foods

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Tea plants have a long cultivation history in the world, but there are few studies on polysaccharides from fresh tea leaves. In this study, tea polysaccharides (TPSs) were isolated from fresh tea leaves. Then, we investigated the characteristics of TPSs during in vitro simulated digestion and fermentation; moreover, the effects of TPSs on gut microbiota were explored. The results revealed that saliva did not significantly affect TPSs’ molecular weight, monosaccharide composition, and reducing sugar content, indicating that TPSs cannot be digested in the oral cavity. However, TPSs were partially decomposed in the gastrointestinal tract after gastric and intestinal digestion, resulting in the release of a small amount of free glucose monosaccharides. Our in vitro fermentation experiments demonstrated that TPSs are degraded by gut microbiota, leading to short-chain fatty acid (SCFA) production and pH reduction. Moreover, TPSs increased the abundance of Bacteroides, Lactobacillus, and Bifidobacterium but reduced that of Escherichia, Shigella, and Enterococcus, demonstrating that TPSs can regulate the gut microbiome. In conclusion, TPSs are partially decomposed by gut microbiota, resulting in the production of SCFAs and the regulation of gut microbiota composition and function. Therefore, TPSs may be used to develop a prebiotic supplement to regulate the gut microbiome and improve host health.

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Section: Changes In Tps Mw and Monosaccharidessupporting

confidence: 91%