Comprehensive Assessment of Nile Tilapia Skin (Oreochromis niloticus) Collagen Hydrogels for Wound Dressings

Abstract

Collagen plays an important role in the formation of extracellular matrix (ECM) and development/migration of cells and tissues. Here we report the preparation of collagen and collagen hydrogel from the skin of tilapia and an evaluation of their potential as a wound dressing for the treatment of refractory wounds. The acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) were extracted and characterized using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), differential scanning calorimetry (DSC), circular dichroism (CD) and Fourier transform infrared spectroscopy (FTIR) analysis. Both ASC and PSC belong to type I collagen and have a complete triple helix structure, but PSC shows lower molecular weight and thermal stability, and has the inherent low antigenicity. Therefore, PSC was selected to prepare biomedical hydrogels using its self-aggregating properties. Rheological characterization showed that the mechanical strength of the hydrogels increased as the PSC content increased. Scanning electron microscope (SEM) analysis indicated that hydrogels could form a regular network structure at a suitable PSC content. Cytotoxicity experiments confirmed that hydrogels with different PSC content showed no significant toxicity to fibroblasts. Skin repair experiments and pathological analysis showed that the collagen hydrogels wound dressing could significantly accelerate the healing of deep second-degree burn wounds and the generation of new skin appendages, which can be used for treatment of various refractory wounds.

A Novel Composite Hydrogel Composed of Formic Acid-Decellularized Pepsin-Soluble Extracellular Matrix Hydrogel and Sacchachitin Hydrogel as Wound Dressing to Synergistically Accelerate Diabetic Wound Healing

“…These results demonstrated that aECM HCl,25 hydrogel was optimal with a sol form at 25 • C for easier application on the wound area and transforming into a gel form at 37 • C with a gel strength higher enough for firmly retaining. Pepsin-soluble collagen (PSC) hydrogel with similar rheological behaviors has been reported by Ge et al [15]. But it did not demonstrate that there exists a sol-gel transition characteristic at 37 • C for PSC hydrogel.…”

“…The "wet wound healing theory" proposes that the wet healing environment is favorable to the granulation growth and to the enablement of the skin cell division, thereby promoting the complete wound healing [15]. Based on this theory, intrinsic characteristics of hydrogels or pastes are expectable to be advantageous for the wound healing process since they provide the dressing with desirable essential properties-better biocompatibility; protection from physical injury; optimal gaseous exchange and nutrients supply to cells due to high sponginess; additionally, their swellability allows for the absorption of excess exudates as well as the maintenance of an optimal moisture environment around the wound, which exhibited to accelerate the wound re-epithelialization process [16].…”

“…Based on this theory, intrinsic characteristics of hydrogels or pastes are expectable to be advantageous for the wound healing process since they provide the dressing with desirable essential properties-better biocompatibility; protection from physical injury; optimal gaseous exchange and nutrients supply to cells due to high sponginess; additionally, their swellability allows for the absorption of excess exudates as well as the maintenance of an optimal moisture environment around the wound, which exhibited to accelerate the wound re-epithelialization process [16]. Hydrogels or pastes based on the natural polymers chitosan [17] and hyaluronic acid [18], an interpenetrated polymer network (IPN) composed of chitosan/oxidized hyaluronic acid/catechol terpolymer/Fe [16], pepsin-soluble collagen isolated from Nile Tilapia skin [15], micronized ADM [19], have been explored for this purpose and demonstrated to provide an extracellular matrix (ECM) mimicking microenvironment with high cell affinity and bioactive functionalities that could enhance the tissue regeneration process and has a great potential for its application as wound dressing.…”

Manufacturing micropatterned collagen scaffolds with chemical-crosslinking for development of biomimetic tissue-engineered oral mucosa

“…Therefore, the collagen scaffold manufactured in this study could facilitate wound healing after transplantation onto wounds in the oral cavity. Applications of marine collagen as a biomimicking material, not only for hard tissues but also soft tissues including hemostats, has great potential in regenerative medicine 52,53 . www.nature.com/scientificreports/ In summary, our manufacturing system is valuable for fabricating a negative mold to replicate a variety of the biomimetic microstructures, such as DEJ on collagen scaffolds, implying competency of this technique to engineer scaffolds with a micropattern composed of a variety of hydrogels, such as the native topography of DEJ.…”

Fish Waste: From Problem to Valuable Resource

“…Collagen from Oreochromis niloticus skin was used to prepare biomedical hydrogel. It has been proven that collagen hydrogel was able to heal of deep second-degree burn of rat skin [ 78 ]. Collagen accelerates wound healing by promoting the expression of vascular endothelial growth factor (VEGF), transforming growth factor-beta (TGF-ß1), basic fibroblast growth factor (bFGF), and alpha-smooth muscle actin (α-SMA) [ 79 ].…”