Comprehensive assessment of the aortic valve in critically ill patients for the non-cardiologist. Part II: Chronic aortic regurgitation of the native valve

Walpot, Jeroen; Vermeiren, Guy L.; Mafragi, Amar Al; Malbrain, Manu L. N. G.

doi:10.5114/ait.2021.104892

Cited by 1 publication

(1 citation statement)

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“…Aortic regurgitation is a valve disease characterized by the inadequate closure of the aortic valve during diastole, resulting in reverse blood flow from the aorta to the left ventricle [7]. Chronic valve regurgitation causes left-ventricle volume overload, leading to a left-ventricle dilatation that remains silent for a long period of time [8]. However, AR patients suddenly progress towards symptoms and HF after this long stable period [9].…”

Section: Introductionmentioning

confidence: 99%

Unraveling the Etiology of Dilated Cardiomyopathy through Differential miRNA–mRNA Interactome

Bonet,

Hernandez-Torres,

Ramos-Sánchez

et al. 2024

Biomolecules

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Dilated cardiomyopathy (DCM) encompasses various acquired or genetic diseases sharing a common phenotype. The understanding of pathogenetic mechanisms and the determination of the functional effects of each etiology may allow for tailoring different therapeutic strategies. MicroRNAs (miRNAs) have emerged as key regulators in cardiovascular diseases, including DCM. However, their specific roles in different DCM etiologies remain elusive. Here, we applied mRNA-seq and miRNA-seq to identify the gene and miRNA signature from myocardial biopsies from four patients with DCM caused by volume overload (VCM) and four with ischemic DCM (ICM). Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis were used for differentially expressed genes (DEGs). The miRNA–mRNA interactions were identified by Pearson correlation analysis and miRNA target-prediction programs. mRNA-seq and miRNA-seq were validated by qRT-PCR and miRNA–mRNA interactions were validated by luciferase assays. We found 112 mRNAs and five miRNAs dysregulated in VCM vs. ICM. DEGs were positively enriched for pathways related to the extracellular matrix (ECM), mitochondrial respiration, cardiac muscle contraction, and fatty acid metabolism in VCM vs. ICM and negatively enriched for immune-response-related pathways, JAK-STAT, and NF-kappa B signaling. We identified four pairs of negatively correlated miRNA–mRNA: miR-218-5p-DDX6, miR-218-5p-TTC39C, miR-218-5p-SEMA4A, and miR-494-3p-SGMS2. Our study revealed novel miRNA–mRNA interaction networks and signaling pathways for VCM and ICM, providing novel insights into the development of these DCM etiologies.

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Section: Introductionmentioning

confidence: 99%