Comprehensive Characterization of a Porcine Model of The “Small-for-Flow” Syndrome

Orue-Echebarria, Maitane I.; Vaquero, Javier; Lisbona, Cristina; Rojas, Pilar; Steiner, M.; Morales, Álvaro; López-Baena, José Ángel; Laso, Juan; Hernández, Inmaculada; Olmedilla, Luis; Sabrido, José Luis García; Peligros, Isabel; Sola, Emma; Carballal, Carlos; Vara, Elena; Asencio, José Manuel

doi:10.1007/s11605-019-04130-2

Cited by 3 publications

(8 citation statements)

References 27 publications

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“…Five studies (12% of all articles) had a non-survival design [34,38,49,53,63]. The average study duration was 13 ± 11 days, ranging from 5 days [46] to 42 days (5%) [54,60], while 7 days was the most used follow-up period (32% of all studies) [30,[39][40][41]47,49,50,55,62,64,65,69] (Table 1).…”

Section: Study Durationmentioning

confidence: 99%

“…Thirty-six studies (88% of all articles) considered hepatic resections, of which 32 were about direct hepatectomy and 4 were ALPPS [44,46,47,55]. The highest RLV was 60% of the primitive liver volume [56,61], while the lowest was 10% [38,49,52,64] (Table 2). Among the ALPPS models, only Croome et al [55] performed an extended-left hepatectomy with a described RLV of 15-20%.…”

Section: Remnant Liver Volume (Rlv) and Surgical Proceduresmentioning

confidence: 99%

“…The most frequently applied resection was the left hepatectomy, with an associated RLV, which ranged from 20% [52] to 50% [35] when data were reported. The right lateral (RL) lobe was involved only in "small-for-size" syndrome models [38,43,49,51,52,57,59,62,64]. Twenty-three studies (64%) reported enough available data to confirm the volume of resected lobes (Table 3), showing the different types of resections with different interpretations of the amount of RLV considered.…”

Section: Remnant Liver Volume (Rlv) and Surgical Proceduresmentioning

confidence: 99%

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Surgical Models of Liver Regeneration in Pigs: A Practical Review of the Literature for Researchers

Cinelli

Muttillo

Felli

et al. 2023

Cells

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The remarkable capacity of regeneration of the liver is well known, although the involved mechanisms are far from being understood. Furthermore, limits concerning the residual functional mass of the liver remain critical in both fields of hepatic resection and transplantation. The aim of the present study was to review the surgical experiments regarding liver regeneration in pigs to promote experimental methodological standardization. The Pubmed, Medline, Scopus, and Cochrane Library databases were searched. Studies evaluating liver regeneration through surgical experiments performed on pigs were included. A total of 139 titles were screened, and 41 articles were included in the study, with 689 pigs in total. A total of 29 studies (71% of all) had a survival design, with an average study duration of 13 days. Overall, 36 studies (88%) considered partial hepatectomy, of which four were an associating liver partition and portal vein ligation for staged hepatectomy (ALPPS). Remnant liver volume ranged from 10% to 60%. Only 2 studies considered a hepatotoxic pre-treatment, while 25 studies evaluated additional liver procedures, such as stem cell application, ischemia/reperfusion injury, portal vein modulation, liver scaffold application, bio-artificial, and pharmacological liver treatment. Only nine authors analysed how cytokines and growth factors changed in response to liver resection. The most used imaging system to evaluate liver volume was CT-scan volumetry, even if performed only by nine authors. The pig represents one of the best animal models for the study of liver regeneration. However, it remains a mostly unexplored field due to the lack of experiments reproducing the chronic pathological aspects of the liver and the heterogeneity of existing studies.

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Section: Study Durationmentioning

confidence: 99%

Section: Remnant Liver Volume (Rlv) and Surgical Proceduresmentioning

confidence: 99%

Section: Remnant Liver Volume (Rlv) and Surgical Proceduresmentioning

confidence: 99%

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Surgical Models of Liver Regeneration in Pigs: A Practical Review of the Literature for Researchers

Cinelli

Muttillo

Felli

et al. 2023

Cells

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“…), and atracurium (0.6 mg/kg i.v. ), following previously described protocols 16 . After the resection of liver mass, all animals were maintained anesthetized for 24 h until the end of the experiments.…”

Section: Anesthetic and Surgical Protocolmentioning

confidence: 99%

“…The measurement of liver function by the indocyanine green retention test and the collection of blood and tissue samples were performed at the beginning of the surgery before deresection of liver mass (Bas), and 15 min (T15m) and 24 h (T24h) after performing the subtotal 90% hepatectomy in conditions of hemodynamic stability. A more detailed description of the present experimental model can be found in a recent publication 16 .…”

Section: Anesthetic and Surgical Protocolmentioning

confidence: 99%

Mecanismos del precondicionamiento regenerativo en hepatectomías subtotales en modelo porcino

Orue-Echebarria¹,

Vaquero²,

Vara³

et al. 2022

CIRU

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Background: Portal vein occlusion shortly before extended hepatic resections has hepatoprotective properties, but its molecular effects have not been elucidated. We characterized the impact of regenerative preconditioning by portal vein embolization (PVE) on hepatic energy metabolism and cytokine expression. Materials and methods: About 90% hepatectomies were performed in normal pigs (Control) and in pigs that underwent a PVE 24 h before the surgery (n = 10/group). Blood biochemistry and coagulation, liver damage, liver function (ICG), hepatic content of adenine nucleotides, and hepatic expression of inflammatory mediators (RT-PCR and WB) were determined before the hepatectomy, 15 min, and 24 h later. Results: All PVE and hepatectomies were successfully accomplished. The 90% hepatectomy resulted in: Immediate reduction of ATP, leading to persistent decreases of energy load and ATP/ADP ratio up to the 24-h time-point; and pro-inflammatory expression profile of cytokines in the remnant liver. Prior performance of PVE attenuated the bioenergetic alterations and prevented many of the changes in hepatic cytokine expression. Conclusions: Regenerative preconditioning by PVE improved hepatic energy metabolism and modulated inflammatory mediators in the remnant liver in pigs undergoing major hepatectomies, potentially contributing to its hepatoprotective effects.

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“Small-for-Flow” Syndrome: Concept Evolution

Orue-Echebarria

Rojas

Olmedilla

et al. 2020

Journal of Gastrointestinal Surgery

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Comprehensive Characterization of a Porcine Model of The “Small-for-Flow” Syndrome

Cited by 3 publications

References 27 publications

Surgical Models of Liver Regeneration in Pigs: A Practical Review of the Literature for Researchers

Surgical Models of Liver Regeneration in Pigs: A Practical Review of the Literature for Researchers

Mecanismos del precondicionamiento regenerativo en hepatectomías subtotales en modelo porcino

“Small-for-Flow” Syndrome: Concept Evolution

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