Comprehensive characterization of human adipose tissue-derived stem cells expanded in vitro

Danišovič, Ľuboš; Kuniaková, Marcela; Varchulová-Nováková, Zuzana; Boháč, Martin; Varga, Ivan; Rusnáková, Jana; Polák, Štefan; Böhmer, Daniel

doi:10.2478/s11756-013-0201-7

Cited by 5 publications

(4 citation statements)

References 27 publications

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“…The first type exhibited the typical ultrastructure of stem cells-nucleus highly rich in euchromatin with multiple indentations and numerous organelles in the cytoplasm. Similar morphological features could be also seen in other types of the stem cells, e.g., adipose tissue-derived stem cells, skeletal muscle-derived stem cells, and dental pulpderived stem cells, as well as in human neonatal fibroblasts after in vitro cultivation [14][15][16]. Interestingly, we have also observed the formation of extracellular vesicles within this type of UDSCs.…”

Section: Discussionsupporting

confidence: 84%

Isolation, Culture and Comprehensive Characterization of Biological Properties of Human Urine-Derived Stem Cells

Culenova

Nicodemou

Nováková

et al. 2021

IJMS

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Mesenchymal stem cells (MSCs) represent an attractive source within the field of tissue engineering. However, their harvesting often requires invasive medical procedures. Urine-derived stem cells (UDSCs) display similar properties to MSCs, and their obtention and further processing is non-invasive for the donors as well as low cost. Here, we offer a comprehensive analysis of their biological properties. The goal of this study was to analyze their morphology, stemness, differentiation potential and cytokine profile. We have successfully isolated UDSCs from 25 urine samples. First colonies emerged up to 9 days after the initial seeding. Cell doubling time was 45 ± 0.24 SD, and when seeded at the density of 100 cells/cm2, they formed 42 ± 6.5 SD colonies within 10 days. Morphological analyzes revealed that two different types of the cell populations have been present. The first type had a rice-grain shape and the second one was characterized by a polyhedral shape. In several cell cultures, dome-shaped cells were observed as well. All examined UDSCs expressed typical MSC-like surface markers, CD73, CD90 and CD105. Moreover, conditioned media from UDSCs were harvested, and cytokine profile has been evaluated showing a significantly higher secretory rate of IL-8, IL-6 and chemokines MCP-1 and GM-CSF. We have also successfully induced human UDSCs into chondrogenic, osteogenic and myogenic cell lineages. Our findings indicate that UDSCs might have immense potential in the regeneration of the damaged tissues.

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Section: Discussionsupporting

confidence: 84%

Isolation, Culture and Comprehensive Characterization of Biological Properties of Human Urine-Derived Stem Cells

Culenova

Nicodemou

Nováková

et al. 2021

IJMS

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“…Their isolation and cultivation was described in our previous works. We were working with muscle-tissue derived stem cells, 12 dental pulp-derived stem cells, 14 adipose tissue-derived stem cells 15 and human mesenchymal stem cells derived from bone marrow. 16 In case of solid tissues we used combination of mechanical disaggregation and enzymatic digestion.…”

Section: Methodsmentioning

confidence: 99%

Ultrastructural analysis of different human mesenchymal stem cells after in vitro expansion: a technical review

et al. 2015

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Transmission electron microscopy reveals ultrastructural details of cells, and it is a valuable method for studying cell organelles. That is why we used this method for detailed morphological description of different adult tissue-derived stem cells, focusing on the morphological signs of their functions (proteosynthetic activity, exchange with external environment, etc.) and their comparison. Preparing a specimen from the cell culture suitable for transmission electron microscopy is, however, much more challenging than routine tissue processing for normal histological examination. There are several issues that need to be solved while working with cell pellets instead of solid tissue. Here we describe a simple protocol for the isolation and culture of mesenchymal stem cells from different adult tissues, with applications to stem cell biology and regenerative medicine. Since we are working with population of cells that was obtained after many days of passaging, very efficient and gentle procedures are highly necessary. We demonstrated that our semi-conservative approach regarding to histological techniques and processing of cells for transmission electron microscopy is a well reproducible procedure which results in quality pictures and images of cell populations with minimum distortions and artifacts. We also commented about riskiest steps and histochemical issues (e.g., precise pH, temperature) while preparing the specimen. We bring full and detailed procedures of fixation, post-fixation, infiltration, embedding, polymerization and contrasting of cell obtained from in vitro cell and tissue cultures, with modifications according to our experience. All this steps are essential for us to know more about adult stem cells derived from different sources or about other random cell populations. The knowledge about detailed ultra-structure of adult stem cells cultured in vitro are also essential for their using in regenerative medicine and tissue engineering.

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“…Mesenchymal stromal cells (MSCs) are a population of multipotent cells [ 1 , 2 ], characterized by a high capacity for self-renewal [ 3 , 4 ] and a high proliferative rate when grown in culture at low density [ 2 , 5 ]. Beyond their proliferative prowess, MSCs exhibit an exceptional regenerative potential [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Influence of the Anatomical Site on Adipose Tissue-Derived Stromal Cells’ Biological Profile and Osteogenic Potential in Companion Animals

Ferreira-Baptista,

Ferreira,

Fernandes

et al. 2023

Veterinary Sciences

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Adipose tissue-derived stromal cells (ADSCs) have generated considerable interest in the field of veterinary medicine, particularly for their potential in therapeutic strategies focused on bone regeneration. These cells possess unique biological characteristics, including their regenerative capacity and their ability to produce bioactive molecules. However, it is crucial to recognize that the characteristics of ADSCs can vary depending on the animal species and the site from which they are derived, such as the subcutaneous and visceral regions (SCAT and VAT, respectively). Thus, the present work aimed to comprehensively review the different traits of ADSCs isolated from diverse anatomical sites in companion animals, i.e., dogs, cats, and horses, in terms of immunophenotype, morphology, proliferation, and osteogenic differentiation potential. The findings indicate that the immunophenotype, proliferation, and osteogenic potential of ADSCs differ according to tissue origin and species. Generally, the proliferation rate is higher in VAT-derived ADSCs in dogs and horses, whereas in cats, the proliferation rate appears to be similar in both cells isolated from SCAT and VAT regions. In terms of osteogenic differentiation potential, VAT-derived ADSCs demonstrate the highest capability in cats, whereas SCAT-derived ADSCs exhibit superior potential in horses. Interestingly, in dogs, VAT-derived cells appear to have greater potential than those isolated from SCAT. Within the VAT, ADSCs derived from the falciform ligament and omentum show increased osteogenic potential, compared to cells isolated from other anatomical locations. Consequently, considering these disparities, optimizing isolation protocols becomes pivotal, tailoring them to the specific target species and therapeutic aims, and judiciously selecting the anatomical site for ADSC isolation. This approach holds promise to enhance the efficacy of ADSCs-based bone regenerative therapies.

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Comprehensive characterization of human adipose tissue-derived stem cells expanded in vitro

Cited by 5 publications

References 27 publications

Isolation, Culture and Comprehensive Characterization of Biological Properties of Human Urine-Derived Stem Cells

Isolation, Culture and Comprehensive Characterization of Biological Properties of Human Urine-Derived Stem Cells

Ultrastructural analysis of different human mesenchymal stem cells after in vitro expansion: a technical review

Influence of the Anatomical Site on Adipose Tissue-Derived Stromal Cells’ Biological Profile and Osteogenic Potential in Companion Animals

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