Comprehensive characterization of naturally occurring antioxidants from the twigs of mulberry (Morus alba) using on‐line high‐performance liquid chromatography coupled with chemical detection and high‐resolution mass spectrometry

Abstract

Introduction: The mulberry tree (Morus alba L.) is a prolific source of biologically active compounds. There is considerable growing interest in probing M. alba twigs as a source of disruptive antioxidant lead candidates for cosmetic skin care product development.Objective: An integrated approach using high-performance liquid chromatography (HPLC) coupled with either chemical detection (CD) or high-resolution mass spectrometry (HRMS) was applied to the hydroalcoholic extract of M. alba to detect and identify lead antioxidant compounds, respectively.

Ionic Liquid−β-Cyclodextrin Vesicle-Based Mechanochemical-Assisted Extraction for the Weak Acid Compounds from Mori Fructus

“…To clearly illustrate the greenness of the established method, we have compared the established method in detail with previously reported extraction methods, − such as solvent extraction and ultrasonic-assisted extraction (UAE). As displayed in Table , the previous methods were similar in operation and organic solvents used (ethanol and methanol), and suffered from long extraction times, tedious operation, and high consumption of samples (10 and 100 g).…”

Extraction of phytochemicals from the pomegranate (Punica granatum L., Punicaceae) by reverse iontophoresis

“…1,2 Characterisation of this plethora of chemicals has advanced signicantly over recent years with respect to increasing sophistication, in terms of sensitivity, specicity, speed and high-throughput capabilities, of analytical and data processing platforms. [2][3][4][5] As a result, exploitation of the vast array of compounds available, whether as nutrition supplements, ingredients in cosmetics and personal care products, or as potential therapeutics, represents an intense eld of research and development activity.…”

Mixed-Valence V2O5-(Ce3+/Ce4+) Metal–Organic-Framework Nanorods for the Colorimetric and Fluorometric Detection of Glutathione

“…Glutathione deficiency is linked to a variety of disorders, including Alzheimer’s, Parkinson’s, liver damage, AIDS, hepatitis, newborn autism, cancer, and others. As a result, the rapid and precise detection of glutathione as a health indicator has gotten a lot of attention. − Currently, capillary electrophoresis, electrochemistry, high-performance liquid chromatography, mass spectrometry, and Raman scattering are all used to detect glutathione levels. However, most of these approaches are difficult, expensive, time-consuming, and have limited sensitivity; as a result, a cost-effective, accurate method is necessary for detecting glutathione levels in cancer cells, as well as other applications in biomedical sciences. , Because of their ease of use, high sensitivity, quick detection, simple and inexpensive equipment, and reliability, spectroscopy techniques, particularly fluorescence and UV–vis, have gotten a lot of attention, , In these two methods, the measurements are based on increasing or decreasing the signal intensity (fluorescence emission intensity or absorption intensity); most UV–vis and fluorescence procedures require specialized enzymes that function as catalysts.…”

“…As a result, the rapid and precise detection of glutathione as a health indicator has gotten a lot of attention. 2−4 Currently, capillary electrophoresis, 5 electrochemistry, 6 high-performance liquid chromatography, 7 mass spectrometry, 8 and Raman scattering 9 are all used to detect glutathione levels. However, most of these approaches are difficult, expensive, time-consuming, and have limited sensitivity; as a result, a cost-effective, accurate method is necessary for detecting glutathione levels in cancer cells, as well as other applications in biomedical sciences.…”