Comprehensive characterization of stress tolerant bacteria with plant growth-promoting potential isolated from glyphosate-treated environment

Zhumakayev, Anuar R.; Vörös, Mónika; Szekeres, András; Rakk, Dávid; Vágvölgyi, Csaba; Szűcs, Attila; Kredics, László; Škrbić, Biljana; Lóránt, Hatvani

doi:10.1007/s11274-021-03065-8

Cited by 10 publications

(11 citation statements)

References 75 publications

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“…All P. resinovorans strains of both genotypes ( Zhumakayev et al., 2021 ) were able to substantially inhibit 6 out of the 7 tested A. tumefaciens and A. vitis ( Table 3 ) strains, however, only partial inhibition was observed in the case of A. vitis . No antagonistic activity was shown by the two Ensifer adhaerens isolates.…”

Section: Resultsmentioning

confidence: 97%

“…Single strains of A. tumefaciens SZMC 14554 and A. vitis SZMC 21784 were not inhibited by any of the P. resinovorans isolates, while A. tumefaciens SZMC 14557 and A. vitis SZMC 21396 were found to be the most susceptible isolates, therefore, they were selected to study their carbon source utilization profiles. Among P. resinovorans strains, SZMC 25872 (genotype I, Zhumakayev et al., 2021 ) showed the most intensive inhibition of A. tumefaciens SZMC 14557 and A. vitis SZMC 21396 ( Table 3 ) and was therefore chosen for detailed characterization in terms of its antagonistic potential in the subsequent assays (Subsection 3.2-3.8).…”

Section: Resultsmentioning

confidence: 99%

“…All strains were grown in LMM supplied with the different compounds (2 g/l) individually to test their ability to utilize them as the sole carbon source. The P. resinovorans SZMC 25872 (genotype I, Zhumakayev et al., 2021 ) strain showed substantial growth (OD 620 >0.2) in the presence of 10 compounds (19, 39, 40, 42, 43, 47, 49, 63, 67, 68). OD 620 values in the case of 9 substances (2, 6, 14, 17, 31, 37, 44, 46, 55) fell in the range of 0.1-0.2, and low growth (OD 620 = 0.05-0.1) was observed in the presence of 10 compounds (7, 10, 12, 13, 16, 20, 34, 35, 45, 73).…”

Section: Resultsmentioning

confidence: 99%

“…Glyphosate-tolerant bacteria were isolated, and identified based on the sequence analysis of fragments of their 16S rRNA and rpoB genes in our previous study ( Zhumakayev et al., 2021 ). Briefly, 5 g of soil sample previously exposed to the glyphosate-containing herbicide “Glifol” (Galenika Fitofarmacija, Belgrade, Serbia) was suspended in 50 ml sterile physiological saline solution (0.9% NaCl in distilled water).…”

Section: Methodsmentioning

confidence: 99%

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Characterization of the antagonistic potential of the glyphosate-tolerant Pseudomonas resinovorans SZMC 25872 strain against the plant pathogenic bacterium Agrobacterium tumefaciens

et al. 2022

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The utilization of microorganisms with biocontrol activity against fungal and bacterial pathogens of plants is recognized as a promising, effective, and environment-friendly strategy to protect agricultural crops. We report the glyphosate-tolerant Pseudomonas resinovorans SZMC 25872 isolate as a novel strain with antagonistic potential towards the plant pathogenic bacterium Agrobacterium tumefaciens. In our studies, the growth of the P. resinovorans SZMC 25872 and A. tumefaciens SZMC 14557 isolates in the presence of 74 different carbon sources, and the effect of 11 carbon sources utilized by both strains on the biocontrol efficacy was examined. Seven variations of media with different carbon sources were selected for the assays to observe the biocontrol potential of the P. resinovorans strain. Also, 50% concentrations of the cell-free culture filtrates (CCF) obtained from medium amended with L-alanine or succinic acid as sole carbon source were found to be effective for the growth suppression of A. tumefaciens by 83.03 and 56.80%, respectively. The effect of 7 media on siderophore amount and the activity of extracellular trypsin- and chymotrypsin-like proteases, as well as esterases were also evaluated. Significant positive correlation was found between the siderophore amount and the percentage of inhibition, and the inhibitory effect of the CCFs obtained from medium amended with succinic acid was eliminated in the presence of an additional iron source, suggesting that siderophores produced by P. resinovorans play an important role in its antagonistic potential. The metabolic profile analysis of the P. resinovorans SZMC 25872 strain, performed by high performance liquid chromatography - high resolution mass spectrometry (HPLC-HRMS), has identified several previously not reported metabolites that might play role in the antagonistic effect against A. tumefaciens. Based on our findings we suggest that the possible inhibition modes of A. tumefaciens SZMC 14557 by P. resinovorans SZMC 25872 include siderophore-mediated suppression, extracellular enzyme activities and novel bioactive metabolites.

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Section: Resultsmentioning

confidence: 97%

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Characterization of the antagonistic potential of the glyphosate-tolerant Pseudomonas resinovorans SZMC 25872 strain against the plant pathogenic bacterium Agrobacterium tumefaciens

et al. 2022

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“…Ensifer adhaerens is a rhizosphere inhabiting bacterium with the ability to genetically transform several plants species (Rudder et al., 2014 ). E. adhaerens strains have been isolated in relation to N 2 fixing (Katiyar et al., 2021 ), biosorption and biodegradation potential (Xu et al., 2016 , Mesa et al., 2017 ), ligninolytic potential (Falade et al., 2017 ), exopolysaccharide production (Marques Alvarez et al., 2018 ), degradation of neonicotinoid insecticides (Sun et al., 2021 , Zhao et al., 2021 ), vitamin B production (Thi Vu et al., 2013 ), mineral weathering capacity (Wang et al., 2016 ), alliinase production with antimicrobial activity (Yutani et al., 2011 ) and growth promotion potential (Zhumakayev et al., 2021 ).…”

Section: Assessmentmentioning

confidence: 99%

Update of the list of QPS‐recommended microbiological agents intentionally added to food or feed as notified to EFSA 16: suitability of taxonomic units notified to EFSA until March 2022

Koutsoumanis¹,

Allende²,

Álvarez‐Ordóñez³

et al. 2022

EFS2

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The qualified presumption of safety (QPS) approach was developed to provide a regularly updated generic pre‐evaluation of the safety of microorganisms, intended for use in the food or feed chains, to support the work of EFSA's Scientific Panels. The QPS approach is based on an assessment of published data for each agent, with respect to its taxonomic identity, the body of relevant knowledge, safety concerns and occurrence of antimicrobial resistance. Safety concerns identified for a taxonomic unit (TU) are, where possible, confirmed at the species/strain or product level and reflected by ‘qualifications’. In the period covered by this statement, no new information was found that would change the status of previously recommended QPS TUs. Of the 50 microorganisms notified to EFSA in October 2021 to March 2022 (inclusive), 41 were not evaluated: 10 filamentous fungi, 1 Enterococcus faecium , 1 Clostridium butyricum, 3 Escherichia coli and 1 Streptomyces spp. because are excluded from QPS evaluation, and 25 TUs that have already a QPS status. Nine notifications, corresponding to seven TUs were evaluated: four of these, Streptococcus salivarius, Companilactobacillus formosensis, Pseudonocardia autotrophica and Papiliotrema terrestris , being evaluated for the first time. The other three, Microbacterium foliorum, Pseudomonas fluorescens and Ensifer adhaerens were re‐assessed. None of these TUs were recommended for QPS status: Ensifer adhaerens, Microbacterium foliorum, Companilactobacillus formosensis and Papiliotrema terrestris due to a limited body of knowledge , Streptococcus salivarius due to its ability to cause bacteraemia and systemic infection that results in a variety of morbidities, Pseudonocardia autotrophica due to lack of body of knowledge and uncertainty on the safety of biologically active compounds which can be produced, and Pseudomonas fluorescens due to possible safety concerns .

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Obtainment and Inoculation of Acinetobacter pittii Strain JJ-2, and Combined Action with Plants for Formaldehyde and CO2 Removal: A Research Study

Zhong,

Chen,

Lin

et al. 2023

Curr Microbiol

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Comprehensive characterization of stress tolerant bacteria with plant growth-promoting potential isolated from glyphosate-treated environment

Cited by 10 publications

References 75 publications

Characterization of the antagonistic potential of the glyphosate-tolerant Pseudomonas resinovorans SZMC 25872 strain against the plant pathogenic bacterium Agrobacterium tumefaciens

Characterization of the antagonistic potential of the glyphosate-tolerant Pseudomonas resinovorans SZMC 25872 strain against the plant pathogenic bacterium Agrobacterium tumefaciens

Update of the list of QPS‐recommended microbiological agents intentionally added to food or feed as notified to EFSA 16: suitability of taxonomic units notified to EFSA until March 2022

Obtainment and Inoculation of Acinetobacter pittii Strain JJ-2, and Combined Action with Plants for Formaldehyde and CO2 Removal: A Research Study

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