Comprehensive collagen crosslinking comparison of microfluidic wet-extruded microfibers for bioactive surgical suture development

Dasgupta, Amrita; Sori, Nardos; Petrova, Stella; Maghdouri‐White, Yas; Thayer, Nick; Kemper, Nathan; Polk, Seth; Leathers, Delaney; Coughenour, Kelly; Dascoli, Jake; Palikonda, Riya; Donahue, Connor; Bulysheva, Anna; Francis, Michael P.

doi:10.1016/j.actbio.2021.04.028

Cited by 25 publications

(12 citation statements)

References 70 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In general, the pure collagen fibers that are physically crosslinked have insufficient mechanical strength, thereby various modification strategies and crosslinking methods have been proposed to overcome this drawback. Dasgupta et al [114] systematically investigated the mechanical properties and biocompatibility of microfluidic collagen microfibers crosslinked by different methods. Fibers were obtained using coaxial needle microfluidics in which the acidic collagen solution was squeezed as an internal phase by an external phase consisting of a neutralizing alkaline solution.…”

Section: Collagenmentioning

confidence: 99%

Biomaterials for microfluidic technology

Chen

Zhang

et al. 2022

Mater. Futures

View full text Add to dashboard Cite

Micro/nanomaterial-based drug and cell delivery systems play an important role in biomedical fields for their injectability and targeting. Microfluidics is a rapidly developing technology and has become a robust tool for preparing biomaterial micro/nanocarriers with precise structural control and high reproducibility. By flexibly designing microfluidic channels and manipulating fluid behavior, various forms of biomaterial carriers can be fabricated using microfluidics, including microspheres, nanoparticles and microfibers. In this review, recent advances in biomaterials for designing functional microfluidic vehicles are summarized. We introduce the application of natural materials such as polysaccharides and proteins as well as synthetic polymers in the production of microfluidic carriers. How the material properties determine the manufacture of carriers and the type of cargoes to be encapsulated is highlighted. Furthermore, the current limitations of microfluidic biomaterial carriers and perspectives on its future developments is presented.

show abstract

Section: Collagenmentioning

confidence: 99%

Biomaterials for microfluidic technology

Chen

Zhang

et al. 2022

Mater. Futures

View full text Add to dashboard Cite

show abstract

“…[15] However, the wet-spinning of collagen fibers, as of yet, has failed to yield nature-like structures, bioactivities, or mechanical strength. [16] Previous studies on the production of collagen fibers out of diluted acids [12,13,[17][18][19][20][21] upon coagulation in pure ethanol, [12] phosphate buffered saline (PBS) buffers, [22] or polyethylene glycol (PEG)-containing buf fers, [11,17,19,21,23] required additional chemical crosslinking using genipin, [13] glutaraldehyde, [13] 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide (EDC/ NHS), [12,24] glyoxal, [23] or formaldehyde [20] to ensure acceptable mechanical properties. While previous studies focused on crosslinking strategies to enhance the mechanical properties of spun fibers, emphasis on determining the effect of these crosslinking processes on host tissue responses was neglected.…”

Section: Introductionmentioning

confidence: 99%

“…[25] Furthermore, insufficient crosslinking can result in lower tensile strength. [23] Delgado et al discussed the response of host tissue and macrophages to different crosslinking methods and densities for stabilizing collagenbased scaffolds. In vitro and in vivo data showed that such chemical crosslinking methods alter the normal wound healing process, even at low concentrations.…”

Section: Introductionmentioning

confidence: 99%

“…[17] Non-crosslinked collagen fibers in a hydrated state are known to be less mechanically stable. [13,23] Further, in most tissue engineering applications such as large-scale musculoskeletal tissue regeneration, collagen-based scaffolds are more common than single fibers. Textile techniques (e.g., weaving and braiding) allow to produce fine-tuned tissuelike constructs mimicking the microarchitecture of the native tissues as well as enhancing the stability of the collagen fibers in wet state.…”

Section: Introductionmentioning

confidence: 99%

“…[ 25 ] Furthermore, insufficient crosslinking can result in lower tensile strength. [ 23 ] Delgado et al. discussed the response of host tissue and macrophages to different crosslinking methods and densities for stabilizing collagen‐based scaffolds.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Processing of Continuous Non‐Crosslinked Collagen Fibers for Microtissue Formation at the Muscle‐Tendon Interface

Koeck

Salehi

Humeník

et al. 2021

Adv Funct Materials

View full text Add to dashboard Cite

One of the main components of the extracellular matrix (ECM) in natural tissues is collagen. Therefore, there has been a strong focus on processing of collagen for biomaterials application in tissue engineering such as in anisotropic tissues like muscles and tendons. To achieve native-like mechanical properties of the in vitro processed collagen, various crosslinking methods have been tested, but the used crosslinkers often do not yield sufficient mechanical properties or induce considerable inflammatory reactions. Here, good mechanical stability of collagen fibers is achieved by self-assembly during wet-spinning without the need of additional crosslinkers. The produced endless collagen fibers show fibril alignment within the fiber with a typical D-band pattern and a periodicity of approximately 67 nm, which is unique for fibril-forming collagens. Furthermore, the collagen fibers are processed into hierarchical assemblies using textile-engineering techniques. The woven assemblies are shown to be excellent substrates for the formation of muscle microtissue with long, aligned, and contractile myofibers. Such constructs are highly important at the muscle-tendon-junction (MTJ) and therefore myoblasts and fibroblasts are co-cultured to develop an MTJ-model.

show abstract

Assembled Cell‐Decorated Collagen (AC‐DC) Fiber Bioprinted Implants with Musculoskeletal Tissue Properties Promote Functional Recovery in Volumetric Muscle Loss

Christensen¹,

Turner

Coughenour³

et al. 2021

Adv Healthcare Materials

Self Cite

View full text Add to dashboard Cite

Musculoskeletal tissue injuries, including volumetric muscle loss (VML), are commonplace and often lead to permanent disability and deformation. Addressing this healthcare need, an advanced biomanufacturing platform, assembled cell-decorated collagen (AC-DC) bioprinting, is invented to rapidly and reproducibly create living biomaterial implants, using clinically relevant cells and strong, microfluidic wet-extruded collagen microfibers. Quantitative analysis shows that the directionality and distribution of cells throughout AC-DC implants mimic native musculoskeletal tissue. AC-DC bioprinted implants further approximate or exceed the strength and stiffness of human musculoskeletal tissue and exceed collagen hydrogel tensile properties by orders of magnitude. In vivo, AC-DC implants are assessed in a critically sized muscle injury in the hindlimb, with limb torque generation potential measured over 12 weeks. Both acellular and cellular implants promote functional recovery compared to the unrepaired group, with AC-DC implants containing therapeutic muscle progenitor cells promoting the highest degree of recovery. Histological analysis and automated image processing of explanted muscle cross-sections reveal increased total muscle fiber count, median muscle fiber size, and increased cellularization for injuries repaired with cellularized implants. These studies introduce an advanced bioprinting method for generating musculoskeletal tissue analogs with near-native biological and biomechanical properties with the potential to repair myriad challenging musculoskeletal injuries.

show abstract

Comprehensive collagen crosslinking comparison of microfluidic wet-extruded microfibers for bioactive surgical suture development

Cited by 25 publications

References 70 publications

Biomaterials for microfluidic technology

Biomaterials for microfluidic technology

Processing of Continuous Non‐Crosslinked Collagen Fibers for Microtissue Formation at the Muscle‐Tendon Interface

Assembled Cell‐Decorated Collagen (AC‐DC) Fiber Bioprinted Implants with Musculoskeletal Tissue Properties Promote Functional Recovery in Volumetric Muscle Loss

Contact Info

Product

Resources

About