Comprehensive depiction of novel heavy metal tolerant and EPS producing bioluminescent Vibrio alginolyticus PBR1 and V. rotiferianus PBL1 confined from marine organisms

Parmar, Paritosh; Shukla, Arpit; Goswami, Dweipayan; Gaur, Shailendra Nath; Patel, Baldev; Saraf, Meenu

doi:10.1016/j.micres.2020.126526

Cited by 18 publications

(5 citation statements)

References 77 publications

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“…It was demonstrated earlier that heavier atoms inhibit bacterial bioluminescence to a higher degree as compared to lighter atoms [ 70 , 71 , 72 ]. This pattern was concerned [ 70 ] with atomic electronegativity, which contributed to efficient electron acceptance by heavy metals, with this providing a basic physicochemical explanation for their multiple effects on complex living systems: interactions with enzymes and substrates, disturbing the free-radical balance in cells and intracellular media, involving ROS balance, and so on.…”

Section: Resultsmentioning

confidence: 99%

Development of Cellular and Enzymatic Bioluminescent Assay Systems to Study Low-Dose Effects of Thorium

et al. 2021

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Thorium is one of the most widespread radioactive elements in natural ecosystems, along with uranium, it is the most important source of nuclear energy. However, the effects of thorium on living organisms have not been thoroughly studied. Marine luminescent bacteria and their enzymes are optimal bioassays for studying low-dose thorium exposures. Luminescent bioassays provide a quantitative measure of toxicity and are characterized by high rates, sensitivity, and simplicity. It is known that the metabolic activity of bacteria is associated with the production of reactive oxygen species (ROS). We studied the effects of thorium-232 (10−11–10−3 M) on Photobacterium phosphoreum and bacterial enzymatic reactions; kinetics of bacterial bioluminescence and ROS content were investigated in both systems. Bioluminescence activation was revealed under low-dose exposures (<0.1 Gy) and discussed in terms of “radiation hormesis”. The activation was accompanied by an intensification of the oxidation of a low-molecular reducer, NADH, during the enzymatic processes. Negative correlations were found between the intensity of bioluminescence and the content of ROS in bacteria and enzyme systems; an active role of ROS in the low-dose activation by thorium was discussed. The results contribute to radioecological potential of bioluminescence techniques adapted to study low-intensity radioactive exposures.

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Section: Resultsmentioning

confidence: 99%

Development of Cellular and Enzymatic Bioluminescent Assay Systems to Study Low-Dose Effects of Thorium

et al. 2021

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“…In this work, 1.04 g EPS as dry weight was obtained from 48 h-cultures associated with the stationary phase of growth. Parmar ( Parmar et al, 2020 ) extracted 1.34% EPS (w/v) but after 72 h at the same temperature (25°C) and concluded that increasing temperature or incubation time decreased EPS yield. Although Daniel ( Daniel et al, 2016 ) proved that Pseudomonas veronii 2E evidenced an optimal biofilm development in PY broth, Ferreira ( Ferreira et al, 2017 ) showed the highest EPS production in a minimal medium with glycerol as sole carbon source and at stationary rather than exponential phase of growth.…”

Section: Discussionmentioning

confidence: 99%

“…One of such adaptations is the ability to produce extracellular polymeric substances (EPS) as a protective barrier which avoids the ERM uptake. The interaction of metals with the EPS matrix usually occurs through an adsorption process as consequence of the presence of negatively charged functional groups ( Parmar et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Metal-Pseudomonas veronii 2E Interactions as Strategies for Innovative Process Developments in Environmental Biotechnology

et al. 2021

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The increase of industrial discharges is the first cause of the contamination of water bodies. The bacterial survival strategies contribute to the equilibrium restoration of ecosystems being useful tools for the development of innovative environmental biotechnologies. The aim of this work was to study the Cu(II) and Cd(II) biosensing, removal and recovery, mediated by whole cells, exopolymeric substances (EPS) and biosurfactants of the indigenous and non-pathogenic Pseudomonas veronii 2E to be applied in the development of wastewater biotreatments. An electrochemical biosensor was developed using P. veronii 2E biosorption mechanism mediated by the cell surface associated to bound exopolymeric substances. A Carbon Paste Electrode modified with P. veronii 2E (CPEM) was built using mineral oil, pre-washed graphite power and 24 h-dried cells. For Cd(II) quantification the CPEM was immersed in Cd(II) (1–25 μM), detected by Square Wave Voltammetry. A similar procedure was used for 1–50 μM Cu(II). Regarding Cd(II), removal mediated by immobilized EPS was tested in a 50 ml bioreactor with 0.13 mM Cd(II), pH 7.5. A 54% metal retention by EPS was achieved after 7 h of continuous operation, while a 40% was removed by a control resin. In addition, surfactants produced by P. veronii 2E were studied for recovery of Cd(II) adsorbed on diatomite, obtaining a 36% desorption efficiency at pH 6.5. Cu(II) adsorption from a 1 mM solution was tested using P. veronii 2E purified soluble EPS in 50 mL- batch reactors (pH = 5.5, 32°C). An 80% of the initial Cu(II) was retained using 1.04 g immobilized EPS. Focusing on metal recovery, Cu nanoparticles (NPs) biosynthesis by P. veronii 2E was carried out in Cu(II)-PYG Broth at 25°C for 5 days. Extracellular CuNPs were characterized by UV-Vis spectral analysis while both extracellular and intracellular NPs were analyzed by SEM and TEM techniques. Responses of P. veronii 2E and its products as biosurfactants, bound and soluble EPS allowed Cu(II) and Cd(II) removal, recovery and biosensing resulting in a multiple and versatile tool for sustainable wastewater biotreatments.

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“…These two kinds of bacteria, PBR1 and PBL1, are luminescent bacteria, subordinate to vibrio genera. Although most of the vibrio strains are pathogenic, the results of a β hemolysin test for PBR1 and PBL1 showed that these two strains are not pathogenic, and that the EPS produced by them can be used to repair heavy metal pollution in the water environment, and that the luminescence characteristics of these two strains can be used to develop luminescent biosensors [ 151 ]. Given the important role of EPS in wastewater treatment, Salama et al proposed five better ways to understand EPS: (1) Development of EPS extraction methods; (2) Establishment of EPS in situ analytical methods; (3) Identifying the sub-fractions of EPS; (4) Elucidation of the key roles of EPS; (5) Identification of the key factors influencing the production of EPS [ 18 ].…”

Section: Environmental Remediationmentioning

confidence: 99%

Exopolysaccharides from Marine Microbes: Source, Structure and Application

Zheng

et al. 2022

Marine Drugs

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The unique living environment of marine microorganisms endows them with the potential to produce novel chemical compounds with various biological activities. Among them, the exopolysaccharides produced by marine microbes are an important factor for them to survive in these extreme environments. Up to now, exopolysaccharides from marine microbes, especially from extremophiles, have attracted more and more attention due to their structural complexity, biodegradability, biological activities, and biocompatibility. With the development of culture and separation methods, an increasing number of novel exopolysaccharides are being found and investigated. Here, the source, structure and biological activities of exopolysaccharides, as well as their potential applications in environmental restoration fields of the last decade are summarized, indicating the commercial potential of these versatile EPS in different areas, such as food, cosmetic, and biomedical industries, and also in environmental remediation.

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Comprehensive depiction of novel heavy metal tolerant and EPS producing bioluminescent Vibrio alginolyticus PBR1 and V. rotiferianus PBL1 confined from marine organisms

Cited by 18 publications

References 77 publications

Development of Cellular and Enzymatic Bioluminescent Assay Systems to Study Low-Dose Effects of Thorium

Development of Cellular and Enzymatic Bioluminescent Assay Systems to Study Low-Dose Effects of Thorium

Metal-Pseudomonas veronii 2E Interactions as Strategies for Innovative Process Developments in Environmental Biotechnology

Exopolysaccharides from Marine Microbes: Source, Structure and Application

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