Comprehensive description of blood microbiome from healthy donors assessed by 16S targeted metagenomic sequencing

Abstract

We demonstrate that a diversified microbiome exists in healthy blood. This microbiome has most likely an important physiologic role and could be implicated in certain transfusion-transmitted bacterial infections. In this regard, the amount of 16S bacterial DNA or the microbiome profile could be monitored to improve the safety of the blood supply.

Circulating microbiome in blood of different circulatory compartments

“…We performed 16S ribosomal RNA (rRNA) gene sequencing of buffy coat samples and identified 65 genera belonging to four phyla (predominantly Proteobacteria) in this cohort (online supplementary figure 1 and figure 1B). Blood microbiome phylum compositions identified in our study agreed with previous findings investigating the peripheral blood microbiome in buffy coat samples from patients with liver fibrosis2 as well as healthy individuals3 but differed from the gut microbiome measured in faecal samples, where Bacteroidetes and Firmicutes are predominant 2…”

Cultural Isolation and Characteristics of the Blood Microbiome of Healthy Individuals

“…In 2016 Paise et al demonstrated that a diversified microbiome exists in healthy blood. Most of the blood bacterial DNA was found located in the BC (93.74%), while RBCs contain more bacterial DNA (6.23%) than the plasma (0.03%) [14]. A significant number of bacterial species were also detected in the blood of healthy chickens [16] …”

“…We note, that some phyla are enriched by the applied resuscitation strategy according to the OTUs combined abundancy (Table 1). Other studies also identified Proteobacteria as predominant in the blood of healthy individuals [14], healthy broilers [16] and cats [17].…”

“…Hypothesis on the bacterial structure of the blood microbiota in healthy humans have been arisen [5] [6] [12], but cultures for exhaustive microbiota analysis remain a target. Rich bacterial diversity in the blood of healthy individuals was confirmed by 16S rRNA genes sequencing [14] and total RNA sequencing [15]. In 2016 Paise et al demonstrated that a diversified microbiome exists in healthy blood.…”

Early-Life Sugar Consumption Affects the Rat Microbiome Independently of Obesity

“…The detection of sequencing fragments was performed by using MiSeq Illumina technology (Illumina Inc.). The 16S targeted sequences were then clustered into operational taxonomic units (OTUs) before taxonomic assignment and analyzed by using the bioinformatics pipeline as described previously (38,39). The OTUs and taxonomy classifications were tabulated, and to account for differences in raw counts across the samples the tables were log normalized by using Equation 1:…”