Constructing an ecological security pattern in ecologically fragile areas is crucial for maintaining regional ecological stability. This study focuses on the Kunlun Mountain region, identifying ecological sources based on habitat suitability assessments and ecosystem services. An ecological resistance evaluation index system is constructed, considering topography, land use, and habitat quality. The minimum cumulative resistance model is then applied to identify ecological corridors, with areas exhibiting higher ecological currents designated as ecological nodes. By integrating the spatial characteristics of ecosystem services, an ecological security pattern is established. The results are as follows: (1) The ecological source area covers approximately 11.30% of the study area. (2) The cumulative length of ecological corridors is 21,111 km, mainly distributed along valleys, gentle slopes, and oasis areas. (3) The areas of ecological nodes and ecological barriers are 126.75 km2 and 46.75 km2, respectively. Ecological nodes are mainly distributed on both sides of the Kunlun Mountains, while ecological barriers are primarily located in the central mountainous area of the Kunlun Mountains. (4) The findings recommend establishing an ecological security pattern consisting of “2 horizontal and 4 vertical corridors and 5 zones” to ensure the ecological security of the Kunlun Mountains. The integration of ecological corridors and ecosystem services in constructing a regional ecological security pattern provides valuable decision-making tools for protecting ecosystems and species in fragile areas.