Comprehensive evaluation of biogenic amines and related drugs’ antiradical activity using reactive 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical

Abstract

Abstract:© Versita Sp. z o.o. Keywords: Biogenic amines • Related drugs • Reference antioxidants • DPPH • Antiradical activityFaculty of Chemistry and Chemical Engineering, România Costel Sârbu, Dorina Casoni * Research ArticleA comprehensive study of biogenic amines and related drugs as free radical scavengers and potential antioxidants was carried out using the stable free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH•). The results of the effect of test duration and the relative concentration on the ability of the compounds to scavenge the DPPH• were investigated and discussed. The antiradical activity of amines was compared with the activity of some reference antioxidants (phenols, phenolic acids, flavonols and other antioxidants). Some particular insights related to the mechanism of reactivity toward the DPPH• were investigated by carrying out kinetic studies in methanol for different relative concentrations. According to the obtained results, catecholamines and especially their related drugs (D-dopa, adrenalone, S-(-)-carbidopa and isoprenaline) showed a fast reactivity towards DPPH• and the highest antiradical activity which increased in a concentration-dependent manner. Moreover, catecholamines proved to be more powerful scavengers than ascorbic and caffeic acids even at low concentrations while their related drugs (D-dopa, adrenalone and S-(-)-carbidopa) showed higher antiradical activity than gallic acid for all of the investigated concentrations.

Comprehensive evaluation of antioxidant activity: A chemometric approach using principal component analysis

“…The activities of biogenic amines neurotransmitters (dopamine (2), norepinephrine (5), epinephrine (6) and their related drug isoprenaline (8) were comparable with the activity of pyrogallol (18) and caffeic acid (20) respectively. All these findings are in good agreement with the results obtained by reference DPPH • method [38].…”

“…The activity order of the investigated antioxidants (quercetin (22) > gallic acid (19) > pyrogallol (18) > caffeic acid (20) > catechol (15) > trolox (24) > ascorbic acid (23) > kaempferol (21) > tocopherol (25)) obtained by using the new proposed parameters (mPC1 and maxPC1), is almost the same as reported by literature [38,41]. By a comparative evaluation, the catecholamine related drug Ddopa (12) is more active than quercetin (22) and adrenalone (10) and carbidopa (13) are more active than gallic acid (19).…”

“…As a consequence, the most recent studies proposed the involvement of different chemometric tools for a complete antioxidant profile evaluation of various classes of compounds [25,27,40]. According to our previous study [38], some important compounds (as biogenic amines and their related drugs) involved in usual metabolic processes proved to have effective in vitro radical scavenging activity. For a comprehensive evaluation of their radical scavenging profiles, in the present study, a new DPPH • spectrophotometric method combined with a chemometric approach was proposed and evaluated.…”

“…Also, for a comparative purpose, in the study, we used a dataset of antioxidant activity evaluation parameters (% RSA and IC 50 values for a set of amines, related drugs and reference antioxidants), experimental determined by us and already published [38].…”

“…In this field efforts are likely to be directed at finding more powerful methods to investigate the free-radical-scavenging profiles and antioxidant properties of existing drugs in order to enhance their applicability as antioxidants [34] and improve their therapeutic applications [35]. According to recent studies, some biogenic amines (important compounds involved in usual metabolic processes and supporting the diagnosis of many diseases) and related drugs proved to have effective in vitro antioxidant and radical scavenging activity [36][37][38] but the complex profiles of these properties are still unclear.…”