This paper calculates the index weights of ecotourism development environmental impact evaluation through AHP hierarchical analysis and constructs a cloud model of ecotourism development environmental impact evaluation by combining the research method of a cloud model so as to prepare for the subsequent example study of an ecotourism development environmental impact evaluation in Nanyang city. Secondly, the impact of tourism development and utilization on the quality of water bodies in the protected area is studied. In order to evaluate the impact of tourism development and utilization on water bodies in the area from various aspects, this paper analyzes the changes in water quality in the protected area from Space-time sequence. Then the above five characteristic parameters are substituted into the MATLAB software forward generator to get the ecotourism development environmental impact evaluation standard cloud model so as to derive the evaluation level of the ecotourism development environmental impact evaluation index located and summarize and analyze the impact of each index. The characteristic parameters of the cloud model are obtained: light impact (0.49.0.166,0.03), average impact (1.49,0.166,0.03), good impact (2.49,0.166.0.03), stronger impact (3.49,0.166,0.03), and strong impact (4.49,0.166,0.03), indicating that the cloud model-based method has the effect of resolving the ambiguity and randomness of comprehensive impact evaluation. This study makes the EIA process transparent and the expression of EIA results intuitive, which can better serve environmental decision-making and management.