Comprehensive expression analysis of Arabidopsis GA2-oxidase genes and their functional insights

Li, Chen; Zheng, Lanlan; Wang, Xuening; Hu, Zhubing; Zheng, Yan; Chen, Qinhua; Hao, Xiao‐Jiang; Xiao, Xiao; Wang, Xuanbin; Wang, Guodong; Zhang, Yonghong

doi:10.1016/j.plantsci.2019.04.023

Cited by 69 publications

(59 citation statements)

References 51 publications

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“…24,25 GA degradation depends on the activity of the GIBBERELLIN 2 OXIDASE (GA2ox) dioxygenases, 26,27 among which GA2ox2 is expressed, as PHB, mostly in the vasculature (SD4). 28 Thus, we hypothesized that PHB might promote GAI stability via the control of GA2ox2 expression.…”

Section: Phb Regulates Ga Homeostasis Via Ga2ox2mentioning

confidence: 99%

A PHABULOSA-Controlled Genetic Pathway Regulates Ground Tissue Patterning in the Arabidopsis Root

Bertolotti¹,

Unterholzner

Scintu³

et al. 2021

Current Biology

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Summary In both animals and plants, development involves anatomical modifications. In the root of Arabidopsis thaliana , maturation of the ground tissue (GT)—a tissue comprising all cells between epidermal and vascular ones—is a paradigmatic example of these modifications, as it generates an additional tissue layer, the middle cortex (MC). 1 , 2 , 3 , 4 In early post-embryonic phases, the Arabidopsis root GT is composed of one layer of endodermis and one of cortex. A second cortex layer, the MC, is generated by asymmetric cell divisions in about 80% of Arabidopsis primary roots, in a time window spanning from 7 to 14 days post-germination (dpg). The cell cycle regulator CYCLIN D6;1 (CYCD6;1) plays a central role in this process, as its accumulation in the endodermis triggers the formation of MC. 5 The phytohormone gibberellin (GA) is a key regulator of the timing of MC formation, as alterations in its signaling and homeostasis result in precocious endodermal asymmetric cell divisions. 3 , 6 , 7 However, little is known on how GAs are regulated during GT maturation. Here, we show that the HOMEODOMAIN LEUCINE ZIPPER III (HD-ZIPIII) transcription factor PHABULOSA (PHB) is a master regulator of MC formation, controlling the accumulation of CYCD6;1 in the endodermis in a cell non-autonomous manner. We show that PHB activates the GA catabolic gene GIBBERELLIN 2 OXIDASE 2 ( GA2ox2 ) in the vascular tissue, thus regulating the stability of the DELLA protein GIBBERELLIN INSENSITIVE (GAI)—a GA signaling repressor—in the root and, hence, CYCD6;1 expression in the endodermis.

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Section: Phb Regulates Ga Homeostasis Via Ga2ox2mentioning

confidence: 99%

A PHABULOSA-Controlled Genetic Pathway Regulates Ground Tissue Patterning in the Arabidopsis Root

Bertolotti¹,

Unterholzner

Scintu³

et al. 2021

Current Biology

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“…These TF genes likely play a crucial role in the regulation of root development and secondary metabolic processes in hexaploid S. canadensis, and the specific functions of the above TF genes in S. canadensis need further exploration. Plant hormones contribute to plant growth and development and some secondary metabolism-related gene regulation; for instance, jasmonic acid regulates terpenoid and flavonoid biosynthesis in plants [62], GA play multiple roles in plant growth and development processes [63], ABA modulates the growth of primary and lateral roots in Arabidopsis [64,65] and flavonoid synthesis in tomato [66]. Furthermore, MAPK signal transduction can combine with hormone signal response processes and develop into regulation network in root [67][68][69].…”

Section: The Expression Of Genes Involved In Regulation May Be Changementioning

confidence: 99%

Metabolome and Transcriptome Analysis of Hexaploid Solidago canadensis Roots Reveals its Invasive Capacity Related to Polyploidy

Chen

et al. 2020

Genes

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Polyploid plants are more often invasive species than their diploid counterparts. As the invasiveness of a species is often linked to its production of allelopathic compounds, we hypothesize that differences in invasive ability between cytotypes may be due to their different ability to synthesize allelopathic metabolites. We test this using two cytotypes of Solidago canadensis as the model and use integrated metabolome and transcriptome data to resolve the question. Metabolome analysis identified 122 metabolites about flavonoids, phenylpropanoids and terpenoids, of which 57 were differentially accumulated between the two cytotypes. Transcriptome analysis showed that many differentially expressed genes (DEGs) were enriched in ‘biosynthesis of secondary metabolites’, ‘plant hormone signal transduction’, and ‘MAPK signaling’, covering most steps of plant allelopathic metabolite synthesis. Importantly, the differentially accumulated flavonoids, phenylpropanoids and terpenoids were closely correlated with related DEGs. Furthermore, 30 miRNAs were found to be negatively associated with putative targets, and they were thought to be involved in target gene expression regulation. These miRNAs probably play a vital role in the regulation of metabolite synthesis in hexaploid S. canadensis. The two cytotypes of S. canadensis differ in the allelopathic metabolite synthesis and this difference is associated with regulation of expression of a range of genes. These results suggest that changes in gene expression may underlying the increased invasive potential of the polyploidy.

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“…The GA20ox6 gene, for example, is essential for reproductive development, including anther dehiscence, pollen fertility, and seed initiation in rice, which is consistent with its expression patterns in rice [31]. Comprehensive expression analysis of Arabidopsis GA2-oxidase genes provides a valuable resource for further elucidating the roles of GA2ox genes during different stages of development [32]. Therefore, the different expression patterns of certain of OsGAox genes in rice indicated that they might play important roles in plant development and have unique functions in specific developmental stages.…”

Section: The Orchestrated Ga Homeostasis Is Quite Complicatedmentioning

confidence: 55%

Genome-wide analysis of gibberellin-dioxygenases genes in rice

Liu¹,

Huang²,

Xu³

et al. 2019

Preprint

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Background Gibberellins (GAs), a pivotal plant hormone, play fundamental roles in plant development and growth. In rice, gibberellin-dioxygenases (GAoxes), OsGA20ox, OsGA3ox and OsGA2ox, are involved in the biosynthesis and deactivation of gibberellins. However, a comprehensive genome-wide analysis of gibberellin-dioxygenases genes is still uncovered.Results In this study, a total of 95 candidate OsGAox genes were found and 19 OsGAox genes were further analyzed. Results of phylogenetic tree showed that the OsGAox genes in Arabidopsis and rice were divided into four subgroups and shared some common features. Furthermore, analysis of gene structure and conserved motifs revealed that splicing phase and motifs were well conserved during the evolution of GAox genes in Arabidopsis and rice, and some specific motifs still need to be further studied. Exploration of expression profiles indicated that most of OsGAox genes exhibited tissue-specific expression patterns, implied their complicated functions. Moreover, the expression patterns of these genes under GA 3 and PAC treatment were investigated, and result showed that some genes, OsGA2ox3, OsGA2ox5, OsGA2ox7, OsGA2ox9, OsGA20ox2, and OsGA20ox4, may play a major role in GA homeostasis to cope with exogenous GA.Conclusions Our study provided a comprehensive analysis of the OsGAox gene family. Splicing characteristics and conserved motifs indicated the evolutionary conserved function in plants. Expression profiles indicated that each OsGAox gene has complicated and special functions. Although plenty of GAoxes were involved in the endogenous GA metabolism, only some of them acted in response to the exogenous GA treatment, which provided available information for researchers to manipulate the chemical GAs to improve the plant architecture and production.

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Comprehensive expression analysis of Arabidopsis GA2-oxidase genes and their functional insights

Cited by 69 publications

References 51 publications

A PHABULOSA-Controlled Genetic Pathway Regulates Ground Tissue Patterning in the Arabidopsis Root

A PHABULOSA-Controlled Genetic Pathway Regulates Ground Tissue Patterning in the Arabidopsis Root

Metabolome and Transcriptome Analysis of Hexaploid Solidago canadensis Roots Reveals its Invasive Capacity Related to Polyploidy

Genome-wide analysis of gibberellin-dioxygenases genes in rice

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