Comprehensive Expression Profiling Analysis of Pituitary Indicates that circRNA Participates in the Regulation of Sheep Estrus

Li, Xiaoyue; Li, Cunyuan; Wei, Junchang; Ni, Wei; Xu, Yuwen; Yao, Rui; Zhang, Mengdan; Li, Huixiang; Liu, Li; Dang, Hanli; Hazi, Wureli; Hu, Shengwei

doi:10.3390/genes10020090

Cited by 25 publications

(34 citation statements)

References 46 publications

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“…During our first estrus observation on the first day of the estrus cycle, three sheep were selected for slaughter in order to collect the anterior pituitary gland. On the other hand, if there is no evidence of estrus after 34-36 days of second estrus cycles, it is called anestrus period [26]. At this stage, three ewes were slaughtered to collect the anterior pituitary, and designated as the anestrus pituitary group (PGA).…”

Section: Sample Collection and Preparationmentioning

confidence: 99%

“…Types of sRNA are determined by the length of the distribution peak. Gene annotation and reference genome were downloaded from sheep reference genome [26]. Using Bowtie software, the sRNA was mapped to a reference sequence.…”

Section: Bioinformatics Analysis Of Small Rna Sequencesmentioning

confidence: 99%

“…The results were presented by cytoscape 3.7.2. Encyclopedia of Genes and Genomes (KEGG) enrichment analysis [32] and the International Standard Classification System for Gene Pathways in Gene Ontology (GO) Analysis [26,33] were used to perform enrichment analysis of differentially expressed miRNAs of two states. Host genes of differentially expressed miRNAs from each group were run for enrichment analysis using GO and KEGG enrichment, respectively, according to the correspondence between miRNA and its host gene.…”

Section: Gene Prediction and Target Enrichment Analysismentioning

confidence: 99%

“…Sheep's seasonal estrus usually occurs in the fall (September-February), followed by the anestrus. The average estrus cycle in sheep is about 16-17 days and the complete estrus cycle in sheep takes approximately 18 days [26].…”

Section: Introductionmentioning

confidence: 99%

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Identification and Profiling of Pituitary microRNAs of Sheep during Anestrus and Estrus Stages

Ullah

et al. 2020

Animals

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MicroRNAs (miRNAs) are a class of small non-coding RNAs, molecules of 21 to 25 nucleotides in length, that regulate gene expression by binding to their target mRNA and play a significant role in animal development. The expression and role of miRNAs in regulating sheep estrus, however, remain elusive. Transcriptome analysis is helpful to understand the biological roles of miRNAs in the pituitary gland of sheep. A sheep’s pituitary gland has a significant difference between estrus and anestrus states. Here, we investigate the expression profiles of sheep anterior pituitary microRNAs (miRNAs) in two states, estrus and anestrus, using Illumina HiSeq-technology. This study identified a total of 199 miRNAs and 25 differentially expressed miRNAs in the estrus and anestrus pituitary gland in sheep. Reverse transcription quantitative-PCR (RT-qPCR) analysis shows six differentially (p < 0.05) expressed miRNAs, that are miR-143, miR-199a, miR-181a, miR-200a, miR-218, and miR-221 in both estrus and anestrus states. miRNAs containing estrus-related terms and pathways regulation are enriched using enrichment analysis from gene ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG). Moreover, we also envisioned a miRNA–mRNA interaction network to understand the function of miRNAs involved in the pituitary gland regulatory network. In conclusion, miRNA expression profiles in sheep pituitary gland in the anestrus and estrus deliver a theoretical basis for the study of pituitary gland biology in sheep.

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Section: Sample Collection and Preparationmentioning

confidence: 99%

Section: Bioinformatics Analysis Of Small Rna Sequencesmentioning

confidence: 99%

Section: Gene Prediction and Target Enrichment Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identification and Profiling of Pituitary microRNAs of Sheep during Anestrus and Estrus Stages

Ullah

et al. 2020

Animals

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“…Research on the hair follicle cycle in the Angora rabbit revealed the existence of a lncRNA/circRNA-miRNA/mRNA network and has shown that non-coding RNAs (ncRNAs) play an important role in regulating the HF cycle [20]. In a another recent study, a total of 12,468 circRNAs and 9,231 differentially-expressed circRNAs were identified in the estrus and anestrus states of the sheep pituitary system [21].…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of circRNAs in the skin during wool follicle development in Aohan fine wool sheep

Zhao

Liu

Han

et al. 2020

Preprint

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Background: Aohan fine wool sheep (AFWS) is a historically bred fine wool sheep, cultivated in China. The wool has excellent quality and good textile performance. Investigating the molecular mechanisms that regulate wool growth is important to improve wool quality and yield. Circular RNAs (circRNAs) are non-coding RNAs that are widely expressed, and can act as a competitive endogenous RNAs (ceRNAs) to bind to miRNAs. Although circRNAs have been studied in many fields, research on their activity in sheep wool follicles is limited. To understand the regulation of circRNAs in the growth of fine wool in sheep, we used RNA-seq to identify circRNAs in sheep shoulder skin samples at three developmental stages: embryonic day 90 (E90d), embryonic day 120 (E120d), and at birth (Birth). Results: We identified 8,753 circRNAs and found that 918 were differentially-expressed. We then analyzed the classification and characteristic of the circRNAs in sheep shoulder skin. Using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG), we identified the source genes of circRNAs, which were mainly enriched in cellular component organization, regulation of primary metabolic processes, tight junctions, and the cGMP-PKG and AMPK signaling pathways. In addition, we predict interactions between 17 circRNAs and eight miRNAs, using miRanda software. Based on the significant pathways, we speculate that circ_0005720, circ_0001754, circ_0008036, circ_0004032, circ_0005174, circ_0005519, circ_0007826 might play an important role in regulating wool follicle growth in AFWS. Seven circRNAs were randomly selected, and have validated the results of the RNA-seq by qRT-PCR. Conclusion: Our results provide more information about circRNAs in regulating wool follicle development in AFWS, and establish a solid foundation for future research.

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Exploring the physiological roles of circular RNAs in livestock animals

Raza

Wijayanti

Pant

et al. 2022

Research in Veterinary Science

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Comprehensive Expression Profiling Analysis of Pituitary Indicates that circRNA Participates in the Regulation of Sheep Estrus

Cited by 25 publications

References 46 publications

Identification and Profiling of Pituitary microRNAs of Sheep during Anestrus and Estrus Stages

Identification and Profiling of Pituitary microRNAs of Sheep during Anestrus and Estrus Stages

Identification and characterization of circRNAs in the skin during wool follicle development in Aohan fine wool sheep

Exploring the physiological roles of circular RNAs in livestock animals

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