Comprehensive gene expression profiling in Japanese flounder kidney after injection with two different formalin-killed pathogenic bacteria

Immune response of flounder (Paralichthys olivaceus) was associated with the concentration of inactivated Edwardsiella tarda and immersion time

“…However, there were gaps to the application of these vaccines in the practice (Tonheim et al, 2008). Nevertheless, the formalin-inactivated E. tarda vaccine was reported to be able to effectively elicit a protective immune response in flounder for a long-term protection by injection, immersion immunization (Gutierrez and Miyazaki, 1994;Kondo et al, 2014). Therefore, the formalin-inactivated E. tarda was still widely employed for the immunological control of edwardsiellosis due to its convenience and cost-effectiveness (Park, 2009).…”

Influence of long (16L:8D) and short (8L:16D) photoperiods on blood metabolites and hepatic metabolism in Olive flounder, Paralichthys olivaceus

“…Specific primers for hsl , pepck and β-actin transcripts were designed using flounder mRNA sequences for hsl (AB828672), β - actin ( AF135499) and pepck using Primer Premier 5.0 software. The sequence of the olive founder pepck gene was obtained from GEO database GSE47556 (Kondo et al 2014 ). Primers were commercially synthesized (Sangon Biotech, Shanghai, China) (Table 1 ), and validation of primers for qRT-PCR were performed using standard ABI protocols.…”

Temperature-dependent regulation of gene expression in poly (I:C)-treated Japanese flounder, Paralichthys olivaceus

“…The cDNA microarray used in this study contained more than 13,000 unique probes [25]. Microarray analysis was carried out using three samples in each group, and each sample was selected based on RNA quality.…”

“…This approach provides valuable information for understanding and discovering gene functions, gene expression patterns, and signal transduction pathways. Consequently, in this study, we performed microarray analysis on the gene expression in Japanese flounder treated with poly (I:C) and reared at 15 C or 25 C using a Japanese flounder oligomicroarray containing more than 13,000 unique probes developed in a recent study [25]. The microarray result was further validated by qPCR.…”