Comprehensive genus-wide screening of seahorse microsatellite loci identifies priority species for conservation assessment

Wilson, Anthony B.; Ashe, Jimiane; Padrón, Mariana; Hamilton, Healy

doi:10.1007/s12686-021-01198-4

Cited by 4 publications

(4 citation statements)

References 39 publications

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“…The guided artificial gene flow strategy was based on genetic analyses of 14 microsatellite loci and enabled the rescue of this species from extinction. All 46 species of seahorses, Hippocampus , are listed as subjects of different types of protection from over-exploitation and are of particular conservation significance [ 169 ]. A set of up to 24 microsatellites has been constructed for cross-species amplification of 15 of species, which will facilitate their further conservation activities.…”

Section: Conservation Geneticsmentioning

confidence: 99%

“…Comparisons of the results of SNP-based and microsatellite-based population studies has often demonstrated the higher accuracy of SNPs. Nevertheless, microsatellites are also effective in the discovery of population processes and kinship analysis and are considered relevant markers in population genetic research [ 14 , 169 , 170 , 171 ].…”

Section: Introductionmentioning

confidence: 99%

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Microsatellites as Molecular Markers with Applications in Exploitation and Conservation of Aquatic Animal Populations

Wenne

2023

Genes

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A large number of species and taxa has been studied for genetic polymorphism. Microsatellites have been known as hypervariable neutral molecular markers with the highest resolution power in comparison with any other markers. However, the discovery of a new type of molecular marker—single nucleotide polymorphism (SNP) has put the existing applications of microsatellites to the test. To ensure good resolution power in studies of populations and individuals, a number of microsatellite loci from 14 to 20 was often used, which corresponds to about 200 independent alleles. Recently, these numbers have tended to be increased by the application of genomic sequencing of expressed sequence tags (ESTs), and the choice of the most informative loci for genotyping depends on the aims of research. Examples of successful applications of microsatellite molecular markers in aquaculture, fisheries, and conservation genetics in comparison with SNPs have been summarized in this review. Microsatellites can be considered superior markers in such topics as kinship and parentage analysis in cultured and natural populations, the assessment of gynogenesis, androgenesis and ploidization. Microsatellites can be coupled with SNPs for mapping QTL. Microsatellites will continue to be used in research on genetic diversity in cultured stocks, and also in natural populations as an economically advantageous genotyping technique.

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Section: Conservation Geneticsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microsatellites as Molecular Markers with Applications in Exploitation and Conservation of Aquatic Animal Populations

Wenne

2023

Genes

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“…Besides the ecological information, conservation planning and management also requires data on population genetic structure, reproductive dynamics and local adaptations (Garner et al 2020). The scarcity of genetic data for threatened species impedes assessing genetic diversity that can provide unique insights into population health, demography and persistence (Wilson et al, 2021). Genetic tools have been used to describe new species and reduce misidentification (Koning and Hoeksema, 2021).…”

Section: Conservationmentioning

confidence: 99%

Distribution of seahorse species in Malaysia, harvesting trends and conservation concerns – A review

Chen¹,

Halid²,

Lim³

et al. 2021

Borneo J. Mar. Sci. Aqua

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Seahorse is a unique fish belonging to the genus Hippocampus. It has a horse-like head, tubular mouth, and the body is covered with rings of armoured plates until the tip of the prehensile tail. Seahorses are traded for human consumption and ornamental purposes. However, the dry product of seahorses has a major demand for traditional Chinese medicine (TCM). The ornamental products such as keychains, earrings and brooches also support a growing ‘curio’ trade. There is a limited amount of published data on seahorse distribution in Malaysia despite increasing pressure of exploitation, this study was carried out to review the available information, identify knowledge gaps and draw attention towards sustainability of the seahorse populations. To date, twelve seahorse species have been reported in Malaysia. Most of these species are found in East Malaysia. In Sabah, 11 out of 12 tropical seahorse species have been encountered. This does not include the Japanese dwarf seahorse, Hippocampus mohnikei. Among the eleven species of seahorses found in Sabah are seven species of common size seahorses, H. barbouri, H. comes, H. histrix, H. kelloggi, H. kuda, H. spinosissimus and H. trimaculatus and four species of pygmy seahorses, H. bargibanti, H. denise, H. satomiae and H. pontohi, that were previously identified as H. severnsi. This study highlights the importance of conservation of wild population of seahorses and the need for farming as a means of lessening the exploitation of wild stocks while meeting the human demand.

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“…As next-generation sequencing technologies offer new opportunities for conservation genetics [ 19 ], microsatellite markers with high mutation rates and genome-wide distributions reveal recent changes in genetic structure and demography critical for population management [ 20 , 21 ]. Although several studies using 10 dinucleotide microsatellites have shown that L. leishanense has high levels of genetic diversity and has not experienced recent bottleneck events [ 22 , 23 , 24 ], genetic assessments of this species are not nearly sufficient.…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity and Population Dynamics of Leptobrachium leishanense (Anura: Megophryidae) as Determined by Tetranucleotide Microsatellite Markers Developed from Its Genome

Cai

et al. 2021

Animals

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Persisting declination of amphibians around the world has resulted in the public attaching importance to the conservation of their biodiversity. Genetic data can be greatly helpful in conservation planning and management, especially in species that are small in size and hard to observe. It is essential to perform genetic assessments for the conservation of Leptobrachium leishanense, an endangered toad and receiving secondary protection on the list of state-protected wildlife in China. However, current molecular markers with low reliability and efficiency hinder studies. Here, we sampled 120 adult toes from the population in the Leishan Mountain, 23 of which were used to develop tetranucleotide microsatellite markers based on one reference L. leishanense genome. After primer optimization, stability detection, and polymorphism detection, we obtained 12 satisfactory microsatellite loci. Then, we used these loci to evaluate the genetic diversity and population dynamics of the 120 individuals. Our results show that there is a low degree of inbreeding in the population, and it has a high genetic diversity. Recently, the population has not experienced population bottlenecks, and the estimated effective population size was 424.3. Accordingly, stabilizing genetic diversity will be key to population sustainability. Recovering its habitat and avoiding intentional human use will be useful for conservation of this species.

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Comprehensive genus-wide screening of seahorse microsatellite loci identifies priority species for conservation assessment

Cited by 4 publications

References 39 publications

Microsatellites as Molecular Markers with Applications in Exploitation and Conservation of Aquatic Animal Populations

Microsatellites as Molecular Markers with Applications in Exploitation and Conservation of Aquatic Animal Populations

Distribution of seahorse species in Malaysia, harvesting trends and conservation concerns – A review

Genetic Diversity and Population Dynamics of Leptobrachium leishanense (Anura: Megophryidae) as Determined by Tetranucleotide Microsatellite Markers Developed from Its Genome

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