Comprehensive identification and expression analysis of Hsp90s gene family in Solanum lycopersicum

Zai, Wen Shan; Miao, Lixiang; Xiong, Zhi Li; Zhang, H L; R, Y; Li, Y L; Chen, Y B; Ye, S G

doi:10.4238/2015.july.14.7

Cited by 18 publications

(12 citation statements)

References 26 publications

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“…Except for mutant analysis, two protein inhibitors (geldanamycin (GDA) and radicicol (RAD)) are also widely used to reveal the functions of HSP90s in plants (Baro et al , ; Queitsch et al , ; Samakovli et al , ; Wang et al , ; Watanabe et al , ). So far, the HSP90 gene family has been reported on a genome‐wide basis in several plant species, including Arabidopsis (Krishna & Gloor, ), Populus trichocarpa (Zhang et al , ), tomato (Zai et al , ), chickpea and pigeonpea (Agarwal et al , ), foxtail millet (Singh et al , ), and Brachypodium distachyon (Zhang et al , ). However, cassava HSP90 genes have not been identified and their functions remain to be further characterized.…”

Section: Introductionmentioning

confidence: 99%

The chaperone MeHSP90 recruits MeWRKY20 and MeCatalase1 to regulate drought stress resistance in cassava

Wei

et al. 2020

New Phytologist

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Summary The 90 kDa heat shock protein (HSP90) is widely involved in various developmental processes and stress responses in plants. However, the molecular chaperone HSP90‐constructed protein complex and its function in cassava remain elusive. In this study, we report that HSP90 is essential for drought stress resistance in cassava by regulating abscisic acid (ABA) and hydrogen peroxide (H2O2) using two specific protein inhibitors of HSP90 (geldanamycin (GDA) and radicicol (RAD)). Among 10 MeHSP90s, the transcript of MeHSP90.9 is largely induced during drought stress. Further investigation identifies MeWRKY20 and MeCatalase1 as MeHSP90.9‐interacting proteins. MeHSP90.9‐, MeWRKY20‐, or MeCatalase1‐silenced plants through virus‐induced gene silencing display drought sensitivity in cassava, indicating that they are important to drought stress response. MeHSP90.9 can promote the direct transcriptional activation of MeWRKY20 on the W‐box element of MeNCED5 promoter, encoding a key enzyme in ABA biosynthesis. Moreover, MeHSP90.9 positively regulates the activity of MeCatalase1, and MeHSP90.9‐silenced cassava leaves accumulate more H2O2 under drought stress. Taken together, we demonstrate that the MeHSP90.9 chaperone complex is a regulator of drought stress resistance in cassava.

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Section: Introductionmentioning

confidence: 99%

The chaperone MeHSP90 recruits MeWRKY20 and MeCatalase1 to regulate drought stress resistance in cassava

Wei

et al. 2020

New Phytologist

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“…Although tomato genome has completely sequenced [23]. Most of the published work in tomato is on Hsp90 [24], Hsp70 [25] and small Hsps [26]. Genome wide analysis of Hsp100 family in tomato has yet to be studied.…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic and expression dynamics of tomato ClpB/Hsp100 gene under heat stress

et al. 2021

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Heat shock proteins (Hsps) are stress-responsive molecular chaperones, which uphold proper protein folding in response to external and internal stresses. The Hsp100 gene family plays a substantial role in thermos-tolerance of plants. This study investigated evolutionary relationship and expression of ClpB/Hsp100 gene family in tomato under heat stress. Six SlHsp100 genes were identified using bioinformatics tools. In silico sub-cellular localization indicated that of these 6 ClpB/Hsp100 members, 4 are found in chloroplast, 1 in mitochondria and 1 in the cytoplasm. For evolutionary study, 36 SlHsp100 genes were included in the phylogenetic tree showing a hierarchical clustering shared by the members of the kingdoms Plantae, Archaea, Chromista, Fungi and Bacteria. A total 4 pairs of orthologous and 5 pairs of paralogous genes were identified. Functional divergence between different Hsp100 clusters showed considerable functional homology. Thermo-tolerance measured in terms of cell viability, cell membrane stability and pollen viability indicated that it was paralleled by thermal resistance of Hsps. Reverse transcriptase polymerase chain reaction was used to analyze gene expression in leaves of five-week-old tomato seedlings following exposure to heat stress (45°C) and control (25°C). Chloroplastic LeHSP110/ClpB gene was upregulated in all tomato genotypes after exposure to heat stress highlighting the crucial role of this gene family in acquired thermo-tolerance.

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“…Recent studies indicated that HSP90s mediate both abiotic and biotic stress resistances in plants [5,[8][9][10], and the expression patterns of HSP90 genes under different biotic and abiotic stress conditions have been widely analysed. HSP90 genes have reported increased expression under heat stress [11] or salt stress [12] in Arabidopsis while being downregulated under cold stress [13] in wheat.…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Characterization of HSP90 Gene Family in Malus sieversii and Their Potential Roles in Response to Valsa mali Infection

Haxim

Liu

et al. 2021

Forests

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Heat shock protein 90 (HSP90) is highly conservative molecular chaperon produced by plants in response to adverse environmental stresses including fungal infection. In China, canker disease, caused by Valsa mali, is the main threat for Malus sieversii, an ancestor of the cultivated apple. In this study, a total of eight HSP90 genes were identified from the M. sieversii genome and randomly distributed on eight chromosomes. According to gene structure and phylogenetic analysis, the MsHSP90s can be divided into five categories. The transcriptome analysis of M. sieversii under V. mali infection showed that the plant pathogen interaction pathway was identified as significantly enriched. RNA-seq data and qRT-PCR analysis demonstrated that the MsHSP90-6a gene was significantly repressed by V. mali infection. We further predicted cis-regulatory elements on the promotor region of MsHSP90 genes and identified canonical SHE motifs. Our results improve our understanding of the HSP90 gene family and provide a foundation for further studies of disease prevention in M. sieversii.

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Comprehensive identification and expression analysis of Hsp90s gene family in Solanum lycopersicum

Cited by 18 publications

References 26 publications

The chaperone MeHSP90 recruits MeWRKY20 and MeCatalase1 to regulate drought stress resistance in cassava

The chaperone MeHSP90 recruits MeWRKY20 and MeCatalase1 to regulate drought stress resistance in cassava

Phylogenetic and expression dynamics of tomato ClpB/Hsp100 gene under heat stress

Genome-Wide Characterization of HSP90 Gene Family in Malus sieversii and Their Potential Roles in Response to Valsa mali Infection

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