2019
DOI: 10.21203/rs.2.11045/v1
|View full text |Cite
Preprint
|
Sign up to set email alerts
|

Comprehensive influences of overexpression of a MYB transcriptor regulating anthocyanin biosynthesis on transcriptome and metabolome of tobacco leaves

Abstract: Background Overexpression of MYB transcription factors can induce the expression of structural genes for anthocyanin biosynthesis and increase the anthocyanin content of plant tissues. However, it remains unclear whether MYB transcription factor overexpression effects the activation of other genes and the concomitant accumulation of chemical compounds. Results Overexpression of LrAN2 promoted anthocyanin accumulation in a variety of tissues in tobacco cultivar Samsun. Only 185 unigenes, from total of 160,96… Show more

Help me understand this report
View published versions

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
2
1

Citation Types

0
9
0

Year Published

2020
2020
2022
2022

Publication Types

Select...
3
3

Relationship

4
2

Authors

Journals

citations
Cited by 14 publications
(9 citation statements)
references
References 23 publications
0
9
0
Order By: Relevance
“…Gene overexpression in Nicotiana tabacum leaves is one such system. Tobacco leaves possess functional genes of the anthocyanin pathway and, probably, a sufficient level of WD40 protein, because no significant differences of its expression are observed between green leaves and anthocyanin-accumulating transgenic tobacco leaves [ 31 ], and the addition of a heterologous WD40 gene also did not observably influence anthocyanin accumulation induced by heterologous MYB and bHLH genes in previous infiltration experiments [ 32 ]. Thus, the expression of relevant MYB and bHLH proteins from infiltrated heterologous genes can lead to the formation of an active MBW complex, which can upregulate native tobacco anthocyanin pathway genes.…”
Section: Resultsmentioning
confidence: 99%
“…Gene overexpression in Nicotiana tabacum leaves is one such system. Tobacco leaves possess functional genes of the anthocyanin pathway and, probably, a sufficient level of WD40 protein, because no significant differences of its expression are observed between green leaves and anthocyanin-accumulating transgenic tobacco leaves [ 31 ], and the addition of a heterologous WD40 gene also did not observably influence anthocyanin accumulation induced by heterologous MYB and bHLH genes in previous infiltration experiments [ 32 ]. Thus, the expression of relevant MYB and bHLH proteins from infiltrated heterologous genes can lead to the formation of an active MBW complex, which can upregulate native tobacco anthocyanin pathway genes.…”
Section: Resultsmentioning
confidence: 99%
“…In transcriptome analysis, expression of the structural genes of anthocyanin biosynthesis was activated in the tuber epidermis of 'QY1', a nding which was also con rmed by qPCR. As is known, expression of the anthocyanin structural genes is regulated by MYB and bHLH transcription factors, with the MYB transcription factors inducing the expression of the bHLH transcription factor [32]. In fact, HtMYB2 was the only MYB transcription factor, regulating anthocyanin biosynthesis, expressed at a high level in the H. tuberosus 'QY1' tuber epidermis, where anthocyanins accumulated, indicating that HtMYB2 was involved in anthocyanin biosynthesis in the tuber epidermis of 'QY1'.…”
Section: Discussionmentioning
confidence: 95%
“…As with the up-regulation of expression of the structural genes in 'QY1', the genes encoding transcription factors MYB and bHLH exhibited greater expression levels in 'QY1' than in 'QY3' ( Table S2). Considering that the structural genes were regulated by the transcription factors, and that the MYB transcription factor could induce expression of the bHLH transcription factor [32]. HtMYB2 (Unigene44371_All) should be the key gene responsible for the red tuber skin color trait in H. tuberosus.…”
Section: Transcriptome Analyses Of Two H Tuberosus Cultivarsmentioning
confidence: 99%
“…In transcriptome analysis, expression of the structural genes of anthocyanin biosynthesis was activated in the tuber epidermis of 'QY1', a finding which was also confirmed by qPCR. As is known, expression of the anthocyanin structural genes is regulated by MYB and bHLH transcription factors, with the MYB transcription factors inducing the expression of the bHLH transcription factor [32]. In fact, HtMYB2 was the only MYB transcription factor expressed at a high level in the H. tuberosus 'QY1' tuber epidermis, where anthocyanins accumulated, indicating that HtMYB2 was involved in anthocyanin biosynthesis in the tuber epidermis of 'QY1'.…”
Section: Discussionmentioning
confidence: 96%