Comprehensive lipid profiling of Microchloropsis gaditana by liquid chromatography - (tandem) mass spectrometry: Bead milling and extraction solvent effects

Cauchie, Gaëla; Delfau-Bonnet, Guillaume; Caulier, Guillaume; Hantson, Anne-Lise; Renault, Jean‐Hugues; Gerbaux, Pascal

doi:10.1016/j.algal.2021.102388

Cited by 6 publications

(2 citation statements)

References 55 publications

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“…Currently, DGTS, MGTS, and DGCC are known to occur in microalgae. For example, DGTS and MGTS in Microchloropsis gaditana , DGCC in Chaetoceros gracilis and Thalassiosira pseudonana , and DGTS in Chlorella sp . have been reported.…”

Section: Resultsmentioning

confidence: 99%

Comprehensive Lipidomic Analysis of Three Edible Microalgae Species Based on RPLC–Q-TOF-MS/MS

Cao,

Cong,

Song

et al. 2024

J. Agric. Food Chem.

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Microalgae, integral to marine ecosystems for their rich nutrient content, notably lipids and proteins, were investigated by using reversed-phase liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (RPLC−Q-TOF-MS/MS). This study focused on lipid composition in three commonly used microalgae species (Spirulina platensis, Chlorella vulgaris, and Schizochytrium limacinum) for functional food applications. The analysis unveiled more than 700 lipid molecular species, including glycolipids (GLs), phospholipids (PLs), sphingolipids (SLs), glycerolipids, and betaine lipids (BLs). GLs (19.9−64.8%) and glycerolipids (24.1−70.4%) comprised the primary lipid. Some novel lipid content, such as acylated monogalactosyldiacylglycerols (acMGDG) and acylated digalactosyldiacylglycerols (acDGDG), ranged from 0.62 to 9.68%. The analysis revealed substantial GLs, PLs, and glycerolipid variations across microalgae species. Notably, S. platensis and C. vulgaris displayed a predominance of fatty acid (FA) 18:2 and FA 18:3 in GLs, while S. limacinum exhibited a prevalence of FA 16:0, collectively constituting over 60% of the FAs of GLs. In terms of PLs and glycerolipids, S. platensis and C. vulgaris displayed elevated levels of arachidonic acid (AA) and eicosapentaenoic acid (EPA), whereas S. limacinum exhibited a significant presence of docosahexaenoic acid (DHA). Principal component analysis (PCA) revealed MGDG (16:0/18:1), DG (16:0/22:5), Cer (d18:1/20:0), and LPC (16:1) as promising lipid markers for discriminating between these microalgae samples. This study contributes to a comprehensive understanding of lipid profiles in three microalgae species, emphasizing their distinct biochemical characteristics and potentially informing us of their high-value utilization in the food industry.

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Section: Resultsmentioning

confidence: 99%

Comprehensive Lipidomic Analysis of Three Edible Microalgae Species Based on RPLC–Q-TOF-MS/MS

Cao,

Cong,

Song

et al. 2024

J. Agric. Food Chem.

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“…Monoacyglyceryl‐ N ‐trimethylhomoserine (MGTS)‐type lipids have only a monoacylglycerol backbone and are thus more polar. To date, they have been studied more rarely, although they are supposed to be common constituents of the membrane lipid bilayers (Cauchie et al, 2021). In addition, some organisms, particularly when exposed to freezing or desiccation, also contain significant amounts of galactolipids with three or more galactose residues, the so‐called oligogalactolipids (OGLs).…”

Section: Introductionmentioning

confidence: 99%

Acquisition of Desiccation Tolerance Unveiled: Polar Lipid Profiles of Streptophyte Algae Offer Insights

Arzac,

Miranda‐Apodaca,

Gasulla

et al. 2023

Physiologia Plantarum

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Terrestrialization by photosynthetic eukaryotes took place in the two branches of green microalgae: Chlorophyta and Charophyta. Within the latter, the paraphyletic streptophytic algae divide into two clades. These are named Klebsormidiophyceae‐Chlorokybophyceae‐Mesostigmatophyceae (KCM), which is the oldest, and Zygnematophyceae‐Coleochaetophyceae‐Charophyceae (ZCC), which contains the closest relatives of vascular plants. Terrestrialization required the emergence of adaptations in response to new challenges, such as irradiance, temperature oscillations and water deprivation. In this study, we evaluated lipid composition in species representative of distinct phylogenetic clusters within Charophyta and Chlorophyta. We aim to study whether the inherent thylakoid lipid composition, as well as its adaptability in response to desiccation, were fundamental factors for the evolutionary history of terrestrial plants. The results showed that the lipid composition was similar to that found in flowering land plants, differing only in betaine lipids. Likewise, the largest constitutive pool of oligogalactolipids (OGL) was found only in the fully desiccation‐tolerant species Klebsormidium nitens. After desiccation, the content of polar lipids decreased in all species. Conversely, the content of OGL increased, particularly trigalactosyldiacylglycerol and tetragalactosyldiacylglycerol in the ZCC clade. The analysis of the molecular species composition of the newly formed OGL may suggest a different biosynthetic route for the KCM and ZCC clades. We speculate that the appearance of a new OGL synthesis pathway, which eventually arose during the streptophyte evolutionary process, endowed algae with a much more dynamic regulation of thylakoid composition in response to stress, which ultimately contributed to the colonization of terrestrial habitats.

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Unwrapping the (glyco-)lipidome in the microalgae Microchloropsis gaditana: Effects of eco-friendly extraction methods

Pühringer,

Rampler,

Castejón

2024

Algal Research

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Comprehensive lipid profiling of Microchloropsis gaditana by liquid chromatography - (tandem) mass spectrometry: Bead milling and extraction solvent effects

Cited by 6 publications

References 55 publications

Comprehensive Lipidomic Analysis of Three Edible Microalgae Species Based on RPLC–Q-TOF-MS/MS

Comprehensive Lipidomic Analysis of Three Edible Microalgae Species Based on RPLC–Q-TOF-MS/MS

Acquisition of Desiccation Tolerance Unveiled: Polar Lipid Profiles of Streptophyte Algae Offer Insights

Unwrapping the (glyco-)lipidome in the microalgae Microchloropsis gaditana: Effects of eco-friendly extraction methods

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