Comprehensive lipidomics of mouse plasma using class-specific surrogate calibrants and SWATH acquisition for large-scale lipid quantification in untargeted analysis

A combined flow injection/reversed phase chromatography – high resolution mass spectrometry workflow for accurate absolute lipid quantification with13C- internal standards

“…Different calibration approaches have been developed minimizing the number of required ISTDs: (1) Class-specific ISTD at a single concentration serve for the quantification of an entire lipid class, [4][5][6][7][8][9][10] or (2) a class-specific ISTD is used at several concentration levels to obtain an internal calibration curve for the whole lipid class. [11][12][13][14] Both calibration approaches are widely accepted for direct infusion mass spectrometry as all standards and analytes co-ionize. On top of that, the streamlined class-specific internal standardization is also applied in combination with chromatographic techniques separating lipids according to their head group chemistry (hydrophilic interaction liquid chromatography HILC, normal phase liquid chromatography NP-LC, supercritical fluid chromatography SFC), in which standards and analytes of the same lipid class co-elute and thus co-ionize.…”

Recent advances in analytical strategies for mass spectrometry-based lipidomics

“…Since comprehensive MS/MS data covering the entire mass range is generated through continuous isolation windows instead of a single wide window, SWATH overperforms all-ion fragmentation method in terms of spectral quality and annotation coverage [ 45 , 55 ]. Therefore, SWATH-based DIA technique has gained widespread popularity in untargeted lipidomics [ [56] , [57] , [58] , [59] ]. However, SWATH encounters a big challenge, i.e., the complexity of MS2 spectra destructs the direct association between precursor ions and fragments [ 60 ].…”

The state of the art in plant lipidomics

“…35 This issue was solved when an open-source data processing software called mass spectrometry-data independent analysis (MS-DIAL) software 101 was introduced to deconvolute the data accumulated by SWATH, which incorporated the LipidBlast 102 library to identify lipids. 101 At present, the SWATH acquisition method is immensely popular for untargeted lipidomic analyses 103 and has been used to analyze lipids in human platelets, 94 mouse plasma, 104 and human plasma. 105 More recently it was adapted for plant lipidomics to analyse lipids from diverse Arabidopsis tissues.…”