Comprehensive metabolic fingerprinting of Withania somnifera leaf and root extracts

Chatterjee, Sandipan; Srivastava, Saurabh Kumar; Khalid, Asna; Singh, Niharika; Sangwan, Rajender S.; Sidhu, O. P.; Roy, Raja; Khetrapal, C. L.; Tuli, Rakesh

doi:10.1016/j.phytochem.2010.04.001

Cited by 194 publications

(113 citation statements)

References 33 publications

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“…These medicinal values are mainly attributed to withanolides present in the roots of W. somnifera [18]. Withanolides are biosynthesized by de novo pathway in roots as well as in leaf [19][20][21]. Quantitative determination of withanolides has been carried out by high-performance liquid chromatography (HPLC) and high-performance thin-layer chromatography (HPTLC) [22][23][24][25].…”

Section: Introductionmentioning

confidence: 99%

Estimation of antioxidant potential of individual components present in complex mixture ofWithania somnifera(Ashwagandha) root fraction by thin-layer chromatography-2,2-diphenyl-1-picrylhdrazyl method

Devkar

Jagtap

Katyare³

et al. 2014

Journal of Planar Chromatography – Modern TLC

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Section: Introductionmentioning

confidence: 99%

Estimation of antioxidant potential of individual components present in complex mixture ofWithania somnifera(Ashwagandha) root fraction by thin-layer chromatography-2,2-diphenyl-1-picrylhdrazyl method

Devkar

Jagtap

Katyare³

et al. 2014

Journal of Planar Chromatography – Modern TLC

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“…Though non-significant, the reducing sugar slightly increased in AVII with progress of storage period, which might be attributed to the hydrolysis of non-reducing sugar. The retention of the determined phytoconstituents in the present study for longer periods (270 days) might be due to the antimicrobial and antioxidative activity of A. racemosus (Alok et al, 2013) and W. somnifera (Chatterjee et al, 2010). Moisture levels in a food material greatly affect its physical, chemical, and microbial stability and have a critical effect on product's shelf-life (Gulati et al, 2015).…”

Section: Organoleptic Evaluationmentioning

confidence: 70%

Development of polyherbal granules and its nutritional characterization

Baruah

Arya

Nalwade

et al. 2017

AJDFR

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Two poly herbal granules (AVII and SBWII) were developed utilizing the extracts of five medicinal plants on the basis of organoleptic evaluation, nutritional composition and shelf life. Five medicinal plants viz., Asparagus racemosus, Chlorophytum borivilianum, Tinospora cordifolia, Tribulus terrestris and Withania somnifera enriched with general tonic and health promoting property were selected for the formulations which were based on Aloe vera gel and soyabean whey. The nutrient analysis of the formulated granules exhibited that the carbohydrates were the major constituents in both AVII and SBWII, followed by protein and total ash. Fat contents were 0.96 and 1.18 g/100g in AVII and SBWII, respectively. The energy levels of AVII and SBWII were 378 and 377 Kcal/100g, correspondingly. Higher contents of Ca (70.20 mg/ 100g), Mg (85.27mg/100g) and P (61.55 mg/100g) were observed in SBWII. The trace elements revealed that, Zn and Fe content were 1.84 and 8.63mg/100g in AVII and 1.85 and 7.85mg/100g in SBWII, respectively. The retention and stability of vitamin C, total sugar, reducing sugar and non-reducing sugar for 270 days illustrated the storage life of the product in ambient condition.

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“…This indicated the presence of O-α-D-glucopyranosyl-β-D-fructofuranosyl-α-D-glucopyranoside and sucrose sugar units were present in the ashwagandha root extract. Further, this is also represented the presence of glyco-withanolides/ withanosides in the root extract [40,48]. Similarly, diethoxy-2-methyl-propane (5) (C 8 H 16 O 2 + ; m/z = 144) was identified at R t of 14.4 minutes ( Figure 2 and Table 2).…”

Section: Figure 1 the Total Ion Chromatograms (Tic) Of The Control Amentioning

confidence: 90%

“…Modern sophisticated techniques such as highperformance liquid chromatography (HPLC) with photodiode array and evaporative light scattering detection, ultraperformance liquid chromatography (UPLC) electrospray ionization (ESI) normally hyphenated with mass spectrometry, gas chromatography (GC), nuclear magnetic resonance (NMR) are very useful for the metabolite profiling and identification of the crude herbal extract [8,[40][41][42]. The LC-MS/MS, GC-MS and NMR analysis of W. somnifera hydro-alcoholic root extract revealed the presence of several known withanolides, alkaloids, glycosides, sugar derivatives, etc.…”

Section: Introductionmentioning

confidence: 99%

Effect of the Energy of Consciousness (The Trivedi Effect<sup>®</sup>) on <i>Withania somnifera</i> Root Extract Using Gas Chromatography – Mass Spectrometry and Nuclear Magnetic Resonance Spectroscopy

Trivedi¹

2017

JDMP

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Abstract:Withania somnifera (Ashwagandha) root extract is very popular ancient herbal medicine. The objective of the study was to characterize and evaluate the impact of The Trivedi Effect ® -Energy of Consciousness Healing Treatment (Biofield Energy Healing) on phytoconstituents present in the ashwagandha root extract using GC-MS and NMR. Ashwagandha root extract was divided into two parts. One part was denoted as the control, while the other part was defined as The Trivedi Effect ® -Biofield Energy Treated sample, which received The Trivedi Effect ® -Energy of Consciousness Healing Treatment remotely from eighteen renowned Biofield Energy Healers. The GC-MS data indicated that the peak height and peak area of The Trivedi Effect ® treated sample were found to be altered compared with the control sample. The peak height of the phytoconstituents present in the treated ashwagandha sample was altered significantly in the range of -8.32% to 89.25% compared with the control sample. Similarly, the peak area of the treated sample was altered significantly in the range of -4.28% to 216.30% compared with the control sample. Overall, the change in the peak area% of the treated sample was significantly altered in the range of -18.29% to 170.18% compared with the control sample. The GC-MS and NMR analysis results identified the presence of withanolides such as glyco-withanolides, alkaloids, and sugars in the root extract in both the sample. The peak area of 2,3,4,5-tetrahydropyridazine (1), methyl ethyl sulfoxide (2), 5,6-dihydro-2-methyl-4(H)pyran-3,4-dione (4), diethoxy-2-methyl-propane (5), 2,3,4,5-tetrahydroxy-tetrahydro-pyran (6), and 3,4-dimethyl-2(3H)-furanone (7) were significantly increased by 170.18%, 58.21%, 7.74%, 139.50%, 23.16%, and 45.63%, respectively in the treated sample compared with the control sample. On the contrary, the peak area% of 2-hydroxy-γ-butyrolactone (3) was decreased by -14.96% in the treated ashwagandha compared with the control sample. From the results, it can be hypothesized that The Trivedi Effect ® -Biofield Energy Treatment might have the impact on the intrinsic physicochemical properties of the 24 Mahendra Kumar Trivedi et al.: Effect of the Energy of Consciousness (The Trivedi Effect ® ) on Withania somnifera Root Extract Using Gas Chromatography -Mass Spectrometry and Nuclear Magnetic Resonance Spectroscopy phytoconstituents present in the ashwagandha root extract and responsible for the alteration in the relative peak height/area of treated sample compared with the control sample. As a result, the concentrations of the phytoconstituents assumed to be increased in treated sample compared with the control sample. This treated ashwagandha root extract would be helpful for designing better nutraceutical/pharmaceutical formulations which might be providing a better therapeutic response against autoimmune diseases, nervous and sexual disorders, infectious diseases, antiaging, diabetes, cancer, immunological disorders, stress, arthritis, etc.

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Comprehensive metabolic fingerprinting of Withania somnifera leaf and root extracts

Cited by 194 publications

References 33 publications

Estimation of antioxidant potential of individual components present in complex mixture ofWithania somnifera(Ashwagandha) root fraction by thin-layer chromatography-2,2-diphenyl-1-picrylhdrazyl method

Estimation of antioxidant potential of individual components present in complex mixture ofWithania somnifera(Ashwagandha) root fraction by thin-layer chromatography-2,2-diphenyl-1-picrylhdrazyl method

Development of polyherbal granules and its nutritional characterization

Effect of the Energy of Consciousness (The Trivedi Effect<sup>®</sup>) on <i>Withania somnifera</i> Root Extract Using Gas Chromatography – Mass Spectrometry and Nuclear Magnetic Resonance Spectroscopy

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Comprehensive metabolic fingerprinting of Withania somnifera leaf and root extracts

Cited by 194 publications

References 33 publications

Estimation of antioxidant potential of individual components present in complex mixture ofWithania somnifera(Ashwagandha) root fraction by thin-layer chromatography-2,2-diphenyl-1-picrylhdrazyl method

Estimation of antioxidant potential of individual components present in complex mixture ofWithania somnifera(Ashwagandha) root fraction by thin-layer chromatography-2,2-diphenyl-1-picrylhdrazyl method

Development of polyherbal granules and its nutritional characterization

Effect of the Energy of Consciousness (The Trivedi Effect&lt;sup&gt;®&lt;/sup&gt;) on &lt;i&gt;Withania somnifera&lt;/i&gt; Root Extract Using Gas Chromatography – Mass Spectrometry and Nuclear Magnetic Resonance Spectroscopy

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Effect of the Energy of Consciousness (The Trivedi Effect<sup>®</sup>) on <i>Withania somnifera</i> Root Extract Using Gas Chromatography – Mass Spectrometry and Nuclear Magnetic Resonance Spectroscopy