Comprehensive metabolite profiling of onion bulbs (Allium cepa) using liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry

Böttcher, Christoph; Krähmer, Andrea; Stürtz, Melanie; Widder, Sabine; Schulz, Hartwig

doi:10.1007/s11306-017-1175-1

Cited by 35 publications

(40 citation statements)

References 38 publications

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“…Overall, quercetin diglucoside (39.7%), quercetin 4- O -glucoside (23.6%), vanillic acid (11.6%), and myricetin (9.9%) were the main phenolic compounds in the nondigested fresh onion samples ( Table 2 ). These results are in line with those of Böttcher et al [ 25 ], who reported, among others, quercetin glycosides with glucosyl moieties in 4′- O and 3- O positions as being the main flavonoids in red and yellow onion cultivars. Regarding black onion, a total of seven polyphenols were identified and quantified, the main type being free quercetin, which accounted for 94% of the total content ( Table 2 ).…”

Section: Resultssupporting

confidence: 93%

Changes in the Organosulfur and Polyphenol Compound Profiles of Black and Fresh Onion during Simulated Gastrointestinal Digestion

Moreno-Ortega

Ordóñez-Díaz

Moreno-Rojas

et al. 2021

Foods

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This study aims to determine the changes in, and bioaccessibility of, polyphenols and organosulfur compounds (OSCs) during the simulated gastrointestinal digestion of black onion, a novel product derived from fresh onion by a combination of heat and humidity treatment, and to compare it with its fresh counterpart. Fresh and black onions were subjected to in-vitro gastrointestinal digestion, and their polyphenol and OSC profiles were determined by ultra-high-performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLC-HRMS). Although to a lesser extent than in the fresh onion, the phenolic compounds in the black variety remained stable during the digestion process, presenting a higher bioaccessibility index (BI) with recovery corresponding to 41.1%, compared with that of fresh onion (23.5%). As for OSCs, apart from being more stable after the digestion process, with a BI of 83%, significantly higher quantities (21 times higher) were found in black onion than in fresh onion, suggesting that the black onion production process has a positive effect on the OSC content. Gallic acid, quercetin, isorhamnetin, and ɣ-glutamyl-S-(1-propenyl)-L-cysteine sulfoxide were the most bioaccessible compounds in fresh onion, while isorhamnetin, quercetin-diglucoside, ɣ-glutamyl-S-methyl-L-cysteine sulfoxide and methionine sulfoxide were found in black onion. These results indicate that OSCs and polyphenols are more bioaccessible in black onion than in fresh onion, indicating a positive effect of the processing treatment.

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Section: Resultssupporting

confidence: 93%

Changes in the Organosulfur and Polyphenol Compound Profiles of Black and Fresh Onion during Simulated Gastrointestinal Digestion

Moreno-Ortega

Ordóñez-Díaz

Moreno-Rojas

et al. 2021

Foods

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“…Metabolite profiling has now been implemented on a broad range of plant species, including but not limited to, tomato, potato, rice, wheat, strawberry, Medicago , Arabidopsis and Allium species . Approximately, 13000 onion accessions are held in gene banks worldwide ( Böttcher et al, 2017 ), but only a small portion of this diverse Allium resource is exploited due to the paucity of information ( Soininen et al, 2014a ; Soininen et al, 2014b ; Soininen et al, 2012 ) . Allium crops are extensively consumed for their broad array of health-promoting effects, which are assigned to the presence of different profiles of organosulfur compounds (OSCs), such asalk(en)yl cysteine sulfoxides, S-allyl cysteine, thiosulfinates (mainly allicin), diallyl sulfides, vinyldithiins, and (E)- and (Z)-ajoene ( Block et al, 2010 ; Ramirez et al, 2017 ).…”

Section: Survey Methodologymentioning

confidence: 99%

“…They also govern important characters in alliums, such as color, pungency, taste, stress tolerance, and medicinal properties. A wide range of modern techniques, such as Liquid Chromatography-Mass Spectrometry (LC–MS), Gas Chromatography–MS (GC-MS), Nuclear Magnetic Resonance (NMR), LC-Fourier Transform Ion Cyclotron Resonance (FTICR)-MS, LC-tandem quadrupole (QqQ)-MS, LC-Quadrupole Time-of-Flight (QTOF)-MS/MS, and Ultrahigh Performance Liquid Chromatography-coupled Electrospray Ionization Quadrupole Time-of-Flight Mass Spectrometry (UHPLC/ESI-QTOFMS), have been used to explore, determine, and characterize the metabolic profile of Allium species ( Soininen et al, 2014a ; Soininen et al, 2014b ; Soininen et al, 2012 ; Nakabayashi et al, 2016 ; Böttcher et al, 2017 ; Molina-Calle et al, 2017 ; Hrbek et al, 2018 ; Table 4 ).…”

Section: Survey Methodologymentioning

confidence: 99%

Omics approaches inAlliumresearch: Progress and way ahead

Khandagale

Krishna

Roylawar

et al. 2020

PeerJ

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Background The genus Allium (Family: Amaryllidaceae) is an economically important group of crops cultivated worldwide for their use as a vegetable and spices. Alliums are also well known for their nutraceutical properties. Among alliums, onion, garlic, leek, and chives cultivated worldwide. Despite their substantial economic and medicinal importance, the genome sequence of any of the Allium is not available, probably due to their large genome sizes. Recently evolved omics technologies are highly efficient and robust in elucidating molecular mechanisms of several complex life processes in plants. Omics technologies, such as genomics, transcriptomics, proteomics, metabolomics, metagenomics, etc. have the potential to open new avenues in research and improvement of allium crops where genome sequence information is limited. A significant amount of data has been generated using these technologies for various Allium species; it will help in understanding the key traits in Allium crops such as flowering, bulb development, flavonoid biosynthesis, male sterility and stress tolerance at molecular and metabolite level. This information will ultimately assist us in speeding up the breeding in Allium crops. Method In the present review, major omics approaches, and their progress, as well as potential applications in Allium crops, could be discussed in detail. Results Here, we have discussed the recent progress made in Allium research using omics technologies such as genomics, transcriptomics, micro RNAs, proteomics, metabolomics, and metagenomics. These omics interventions have been used in alliums for marker discovery, the study of the biotic and abiotic stress response, male sterility, organ development, flavonoid and bulb color, micro RNA discovery, and microbiome associated with Allium crops. Further, we also emphasized the integrated use of these omics platforms for a better understanding of the complex molecular mechanisms to speed up the breeding programs for better cultivars. Conclusion All the information and literature provided in the present review throws light on the progress and potential of omics platforms in the research of Allium crops. We also mentioned a few research areas in Allium crops that need to be explored using omics technologies to get more insight. Overall, alliums are an under-studied group of plants, and thus, there is tremendous scope and need for research in Allium species.

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“…For this purpose, an Infinity 1290 Series UHPLC system interfaced via a dual Agilent jet stream electrospray ion source to an iFunnel Q-TOF mass spectrometer (G6550A, Agilent Technologies, Santa Clara, CA, USA) was used. For further instrument settings see Böttcher et al [32].…”

Section: Treatment Numbermentioning

confidence: 99%

Do Soil Warming and Changes in Precipitation Patterns Affect Seed Yield and Seed Quality of Field-Grown Winter Oilseed Rape?

et al. 2020

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Increasing air and soil temperatures and changes in precipitation patterns as consequences of climate change will affect crop production in agricultural ecosystems. The combined effects of soil warming and altered precipitation on the productivity and product quality of oil crops are not yet well studied. Winter oilseed rape (OSR) (Brassica napus L., cv. Mercedes) was field-grown under elevated soil temperature (+2.5 °C), reduced precipitation amount (−25%), reduced precipitation frequency (−50%) both separately and in combination in order to investigate effects on crop development, seed yield, and seed quality. Soil warming accelerated crop development during early plant growth and during spring. At maturity, however, plants in all treatments were similar in quantitative (aboveground biomass, seed yield) and qualitative (protein and oil content, amino acids, fatty acids) parameters. We observed the long-term effects of the precipitation manipulation on leaf size, leaf senescence and biomass allocation. Seed yield was not affected by the altered climatic factors, perhaps due to adaptation of soil microorganisms to permanent soil warming and to relatively wet conditions during the seed-filling period. Overall, OSR performed well under moderate changes in soil temperature and precipitation patterns; thus, we observed stable seed yield without negative impacts on nutritive seed quality.

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Comprehensive metabolite profiling of onion bulbs (Allium cepa) using liquid chromatography coupled with electrospray ionization quadrupole time-of-flight mass spectrometry

Cited by 35 publications

References 38 publications

Changes in the Organosulfur and Polyphenol Compound Profiles of Black and Fresh Onion during Simulated Gastrointestinal Digestion

Changes in the Organosulfur and Polyphenol Compound Profiles of Black and Fresh Onion during Simulated Gastrointestinal Digestion

Omics approaches inAlliumresearch: Progress and way ahead

Do Soil Warming and Changes in Precipitation Patterns Affect Seed Yield and Seed Quality of Field-Grown Winter Oilseed Rape?

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