Comprehensive Multiphase (CMP) NMR Monitoring of the Structural Changes and Molecular Flux Within a Growing Seed

Fortier-McGill, Blythe; Majumdar, Rudraksha Dutta; Lam, Leayen; Soong, Ronald; Liaghati-Mobarhan, Yalda; Sutrisno, Andre; Visser, R.G.F.; Simpson, Myrna J.; Wheeler, Heather L.; Campbell, Malcolm M.; Gorissen, A.; Simpson, André J.

doi:10.1021/acs.jafc.7b02421

Cited by 27 publications

(30 citation statements)

References 42 publications

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“…Resonances consistent with omega‐3 fatty acids along with triacylglycerides are among the most dominant components in this fraction . Figure c represents the relaxation recovery arising from diffusion editing spectrum, most consistent with “semisolids” and strong contribution from lipids, likely due to faster relaxing components in membranes and cell walls . In addition, signals from aromatic amino acids are noted, consistent with proteins existing within rigid tissues such as muscle.…”

Section: Experimental Considerationsmentioning

confidence: 82%

“…13 C NMR is a simple and effective approach to obtain metabolic information in vivo and does not require water suppression. In this case, as only low‐power decoupling was used, the experiment will bias 13 C attached to protons with relatively narrow line shape (i.e., liquids and gels) and discriminate against true solids . If absolute quantification is required with each phase being represented equally, then the recently published approach of stepped decoupling, specifically developed for 13 C quantification in multiphase samples, should be employed …”

Section: Future Potential and Heteronuclear Nmrmentioning

confidence: 99%

“…In many ways, CMP NMR can be thought of as changing NMR technology to match natural samples, rather than changing the sample to match a specific NMR technique. CMP NMR has permitted the study of structural interactions and processes in unaltered heterogeneous samples, including soil, swelling of rubber by biofuels, intact plants, and to follow biological processes (seed growth) …”

Section: Introductionmentioning

confidence: 99%

“…Since then CMP NMR has also been applied to monitor D . magna and seed growth but its application to living systems is still very much in its infancy.…”

Section: Introductionmentioning

confidence: 99%

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In vivo comprehensive multiphase NMR

Mobarhan

Soong

Lane

et al. 2019

Magnetic Reson in Chemistry

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Traditionally, due to different hardware requirements, nuclear magnetic resonance (NMR) has developed as two separate fields: one dealing with solids, and one with solutions. Comprehensive multiphase (CMP) NMR combines all electronics and hardware (magic angle spinning [MAS], gradients, high power Radio Frequency (RF) handling, lock, susceptibility matching) into a universal probe that permits a comprehensive study of all phases (i.e., liquid, gel‐like, semisolid, and solid), in intact samples. When applied in vivo, it provides unique insight into the wide array of bonds in a living system from the most mobile liquids (blood, fluids) through gels (muscle, tissues) to the most rigid (exoskeleton, shell). In this tutorial, the practical aspects of in vivo CMP NMR are discussed including: handling the organisms, rotor preparation, sample spinning, water suppression, editing experiments, and finishes with a brief look at the potential of other heteronuclei (2H, 15N, 19F, 31P) for in vivo research. The tutorial is aimed as a general resource for researchers interested in developing and applying MAS‐based approaches to living organisms. Although the focus here is CMP NMR, many of the approaches can be adapted (or directly applied) using conventional high‐resolution magic angle spinning, and in some cases, even standard solid‐state NMR probes.

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Section: Experimental Considerationsmentioning

confidence: 82%

Section: Future Potential and Heteronuclear Nmrmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

“…Since then CMP NMR has also been applied to monitor D . magna and seed growth but its application to living systems is still very much in its infancy.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

In vivo comprehensive multiphase NMR

Mobarhan

Soong

Lane

et al. 2019

Magnetic Reson in Chemistry

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“…In some of its earliest applications, CMP-NMR technology has been applied to identify the complete metabolic and structural profile of intact 13 C-labelled seeds [ 142 ]. This was followed by a second study which focused on the growth of the seeds and followed all the components during germination and early stages of development [ 145 ]. In 2016, CMP-NMR was applied to the living organisms H. azteca (freshwater shrimp) [ 46 ], an organism commonly used in aquatic toxicity testing, that swims and burrows into sediments, thus providing information on both water and sediment contamination [ 146 ].…”

Section: Types Of Nmrmentioning

confidence: 99%

In-Vivo NMR Spectroscopy: A Powerful and Complimentary Tool for Understanding Environmental Toxicity

et al. 2018

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Part review, part perspective, this article examines the applications and potential of in-vivo Nuclear Magnetic Resonance (NMR) for understanding environmental toxicity. In-vivo NMR can be applied in high field NMR spectrometers using either magic angle spinning based approaches, or flow systems. Solution-state NMR in combination with a flow system provides a low stress approach to monitor dissolved metabolites, while magic angle spinning NMR allows the detection of all components (solutions, gels and solids), albeit with additional stress caused by the rapid sample spinning. With in-vivo NMR it is possible to use the same organisms for control and exposure studies (controls are the same organisms prior to exposure inside the NMR). As such individual variability can be reduced while continual data collection over time provides the temporal resolution required to discern complex interconnected response pathways. When multidimensional NMR is combined with isotopic labelling, a wide range of metabolites can be identified in-vivo providing a unique window into the living metabolome that is highly complementary to more traditional metabolomics studies employing extracts, tissues, or biofluids.

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Evaluation of double‐tuned single‐sided planar microcoils for the analysis of small ¹³C enriched biological samples using ¹H‐¹³C 2D heteronuclear correlation NMR spectroscopy

Moxley-Paquette

Lane

et al. 2021

Magnetic Reson in Chemistry

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Microcoils provide a cost-effective approach to improve detection limits for mass-limited samples. Single-sided planar microcoils are advantageous in comparison to volume coils, in that the sample can simply be placed on top. However, the considerable drawback is that the RF field that is produced by the coil decreases with distance from the coil surface, which potentially limits more complex multi-pulse NMR pulse sequences. Unfortunately, 1 H NMR alone is not very informative for intact biological samples due to line broadening caused by magnetic susceptibility distortions, and 1 H-13 C 2D NMR correlations are required to provide the additional spectral dispersion for metabolic assignments in vivo or in situ. To our knowledge, double-tuned single-sided microcoils have not been applied for the 2D 1 H-13 C analysis of intact 13 C enriched biological samples. Questions include the following: Can 1 H-13 C 2D NMR be performed on single-sided planar microcoils? If so, do they still hold sensitivity advantages over conventional 5 mm NMR technology for mass limited samples? Here, 2D 1 H-13 C HSQC, HMQC, and HETCOR variants were compared and then applied to 13 C enriched broccoli seeds and Daphnia magna (water fleas). Compared to 5 mm NMR probes, the microcoils showed a sixfold improvement in mass sensitivity (albeit only for a small localized region) and allowed for the identification of metabolites in a single intact D. magna for the first time. Single-sided planar microcoils show practical benefit for 1 H-13 C NMR of intact biological samples, if localized information within $0.7 mm of the 1 mm I.D. planar microcoil surface is of specific interest.

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Comprehensive Multiphase (CMP) NMR Monitoring of the Structural Changes and Molecular Flux Within a Growing Seed

Cited by 27 publications

References 42 publications

In vivo comprehensive multiphase NMR

In vivo comprehensive multiphase NMR

In-Vivo NMR Spectroscopy: A Powerful and Complimentary Tool for Understanding Environmental Toxicity

Evaluation of double‐tuned single‐sided planar microcoils for the analysis of small ¹³C enriched biological samples using ¹H‐¹³C 2D heteronuclear correlation NMR spectroscopy

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Comprehensive Multiphase (CMP) NMR Monitoring of the Structural Changes and Molecular Flux Within a Growing Seed

Cited by 27 publications

References 42 publications

In vivo comprehensive multiphase NMR

In vivo comprehensive multiphase NMR

In-Vivo NMR Spectroscopy: A Powerful and Complimentary Tool for Understanding Environmental Toxicity

Evaluation of double‐tuned single‐sided planar microcoils for the analysis of small 13C enriched biological samples using 1H‐13C 2D heteronuclear correlation NMR spectroscopy

Contact Info

Product

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Evaluation of double‐tuned single‐sided planar microcoils for the analysis of small ¹³C enriched biological samples using ¹H‐¹³C 2D heteronuclear correlation NMR spectroscopy