2006
DOI: 10.1073/pnas.0510740103
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Comprehensive mutation identification in an evolved bacterial cooperator and its cheating ancestor

Abstract: Precise characterization of the mutation histories of evolutionary lineages is crucial for understanding the evolutionary process, yet mutation identification has been constrained by traditional techniques. We sought to identify all accumulated mutations in an experimentally evolved lineage of the cooperative bacterium Myxococcus xanthus, which constructs fruiting bodies by a process of social multicellular development in response to starvation. This lineage had undergone two major transitions in social phenot… Show more

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Cited by 139 publications
(121 citation statements)
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“…At least one mutation in the LTEE was necessary to produce a genetic background with the potential to generate Cit ϩ variants, while the distribution and dynamics of Cit ϩ mutants in fluctuation tests indicate at least two additional mutations are involved. To find the relevant mutations, we will perform whole-genome resequencing, which has become a powerful approach that is well suited to experimental evolution (57)(58)(59). We expect to find dozens of mutations relative to the ancestor (22), which will complicate identification of those changes that were important specifically for the origin of the Cit ϩ function.…”
Section: Discussion and Future Directionsmentioning
confidence: 99%
“…At least one mutation in the LTEE was necessary to produce a genetic background with the potential to generate Cit ϩ variants, while the distribution and dynamics of Cit ϩ mutants in fluctuation tests indicate at least two additional mutations are involved. To find the relevant mutations, we will perform whole-genome resequencing, which has become a powerful approach that is well suited to experimental evolution (57)(58)(59). We expect to find dozens of mutations relative to the ancestor (22), which will complicate identification of those changes that were important specifically for the origin of the Cit ϩ function.…”
Section: Discussion and Future Directionsmentioning
confidence: 99%
“…This multi-level understanding facilitates the interpretation of evolution. It is also now possible to obtain genome-wide sequences of bacteria to identify evolutionary changes (Honisch et al, 2004;Herring et al, 2006;Velicer et al, 2006) and to obtain genome-wide expression levels (Fong et al, 2005b). At the same time, explaining the evolutionary changes can be problematic.…”
Section: Experimental Evolution In Free-living Organismsmentioning
confidence: 99%
“…This strain could form spores, resist the cheater mutant, and even produced more spores than the ancestral (wild-type) strain. Whole-genome sequencing revealed that it took just a single base change to go from the cheater strain to the new super strain [63]. The mutation increases expression of a gene that is predicted to encode a member of the Gcn5-related N-acetyltransferase superfamily, some members of which regulate both global and specific gene expression [64].…”
Section: Phoenix Genesmentioning
confidence: 99%
“…This goal is helped by the small size of many microbial genomes and by the emergence of economical sequencing methods [63]. One hypothesis is that genes that are involved in competitive interactions undergo rapid evolution owing to evolutionary arms races [66,67].…”
Section: Social-gene Evolution In Naturementioning
confidence: 99%