Comprehensive on-line RPLC-CZE-MS of peptides

Lewis, Kenneth C.; Opiteck, Gregory J.; Jorgenson, James W.; Sheeley, Douglas M.

doi:10.1016/s1044-0305(97)00009-3

Cited by 95 publications

(95 citation statements)

References 18 publications

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“…However, if the ultimate in sensitivity is required the sheathless design is preferred due to its higher ionization efficiency in this nanospray design [15]. Attempts have been made to optimize all interfaces: By reducing the sheath liquid flow the ionization efficiency has been tried to increase [16][17][18], however, "nonstandard" capillaries (OD 180 mm) are required. On the other hand the lifespan of the capillary can be increased by using capillary tip coatings based on conductive polymers [19].…”

Section: Esimentioning

confidence: 99%

Quantitation in capillary electrophoresis-mass spectrometry

2005

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Quantitation in capillary electrophoresis-mass spectrometryCE-MS has evolved into a strong alternative to LC-MS. Most of CE-MS applications deal with characterization and identification. However, quantitative aspects have gained importance in, e.g., pharmaceutical and biotechnological applications. Here we summarize and evaluate various methodological aspects in order to achieve sensitive and reproducible results. Similar to LC-MS, aspects of matrix influence on the electrospray process need to be carefully addressed when quantitative results are intended by CE-MS. Due to a more complicated coupling special emphasis needs to be put on the CE-MS interface. Generally linearity over more than three orders of magnitude can be achieved by CE-ESI-MS. Furthermore, a literature survey has been performed in order to give an overview over quantitative measurements performed by CE-MS. The precision can be doubled when changing from a structural related to an isotopically labeled internal standard. Thus a level of precision better than 5% RSD can be achieved.

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Section: Esimentioning

confidence: 99%

Quantitation in capillary electrophoresis-mass spectrometry

2005

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“…The interface was transparent and allowed direct observation of the transfer of sample from LC to CZE. A 3-D system was further developed by coupling the 2-D system of RPLC-CZE with MS [46]. Peptide standard and tryptic digests of ribonuclease B were separated and detected on-line by electrospray mass spectrometry in about 15 min.…”

Section: Ce-cementioning

confidence: 99%

“…In contrast to 2-D PAGE, multi-dimensional liquid chromatography has emerged with obvious advantages of availability of various chromatographic modes offering different separation mechanisms, automation, high resolution, sensitivity, reproducibility, and high throughput. Multidimensional separation techniques, including liquid chromatography-liquid chromatography (LC-LC) [1 -3], capillary electrophoresis-capillary electrophoresis (CE-CE) [4,5], liquid chromatography-capillary electrophoresis (LC-CE) [6,7], etc., have attracted much attention in recent years. They have been widely used for separation of biological samples [8,9], polymers [10], TCMs [11,12], and other complex mixtures [13].…”

Section: Introductionmentioning

confidence: 99%

Recent developments and contributions from Chinese scientists in multidimensional separations for proteomics and traditional Chinese medicines

Gao

Deng

Lin

et al. 2007

J of Separation Science

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ReviewRecent developments and contributions from Chinese scientists in multidimensional separations for proteomics and traditional Chinese medicinesThe most basic task in proteomics remains the detection and identification of proteins from a biological sample, and the most traditional way to achieve this goal consists in protein separations performed by two-dimensional polyacrylamide gel electrophoresis (2-D PAGE). Yet the 2-D PAGE-mass spectrometry (MS) approach has its drawbacks with regard to automation, sensitivity, and throughput. Consequently, considerable effort has been devoted to the development of non-gel-based proteome separation technologies in an effort to alleviate the shortcomings of 2-D PAGE. In addition, traditional Chinese medicines (TCMs), due to their long period of clinical testing and reliable therapeutic efficacy, are attracting increased global attention. However, hundreds or even thousands of components are usually present in TCMs, which results in great difficulties of separation. As a mainstream separation tool, multidimensional liquid separation systems have shown powerful separation ability, high peak capacity, and excellent detectability in the analysis of complex samples including biological samples and TCMs, etc. Therefore, this review emphasizes the most recent advances in multidimensional liquid chromatography and capillary electrophoresis-based separation techniques, and the corresponding applications in proteomics and TCMs. In view of the significant contributions from Chinese scientists, this review focuses mainly on the work of Chinese scientists in the above fields.

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“…In addition to implementation of electric connection for CE, the interface should have the ability to exactly transfer nanoliters of fractions from SPE onto CE with minimized degradation of the efficiencies of SPE and CE. For such a purpose, a variety of interfaces have been designed, including such types as valve-loop, 7,8 T-split, 9,10 valve-loop-vial or T-piece, [11][12][13][14] two-leveled two cross, 15,16 flow-gating, 11,[17][18][19][20][21] and interfaces with integrated SPE. 22,23 With the first three types of interfaces, success of the coupling has been achieved to some extent, but some limitations have been recognized.…”

Section: Introductionmentioning

confidence: 99%

A Miniaturized Transverse Flow Gating Interface for the On-line Coupling of Solid-phase Extraction with Capillary Electrophoresis

Weng

et al. 2014

ANAL. SCI.

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A miniaturized transverse flow gating interface (μ-TFGI) is presented for the on-line coupling of solid-phase extraction with capillary electrophoresis (SPE-CE). The fabrication and operation procedures of the μ-TFGI are described in detail. After the operation conditions were investigated and selected, the μ-TFGI was evaluated on an SPE-CE-UV setup using clenbuterol (CLB) as the test analyte. The preconcentration factors obtained with the SPE-CE-UV system and the SPE-UV part were 600 and 620, respectively. The plate numbers obtained within 3 min were 100000 and 80000 for automatic injection via the μ-TFGI and manual direct injection, respectively. The precisions were 2.4 -6.8% (RSD, %, n = 9) and 3.1% (RSD, %, n = 27) for the recovery (94.3 -101.9%) and migration time of CLB spiked in urine samples, respectively. These results demonstrate that the μ-TFGI has the ability to exactly and reproducibly transfer nanoliters of fractions from SPE onto CE with no degradation of the efficiencies of SPE and CE.

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Comprehensive on-line RPLC-CZE-MS of peptides

Cited by 95 publications

References 18 publications

Quantitation in capillary electrophoresis-mass spectrometry

Quantitation in capillary electrophoresis-mass spectrometry

Recent developments and contributions from Chinese scientists in multidimensional separations for proteomics and traditional Chinese medicines

A Miniaturized Transverse Flow Gating Interface for the On-line Coupling of Solid-phase Extraction with Capillary Electrophoresis

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