Comprehensive phenotyping of human peripheral blood B lymphocytes in healthy conditions

Abstract

The B cell compartment provides innate and adaptive immune defenses against pathogens. Different B cell subsets, reflecting the maturation stages of B cells, have noninterchangeable functions and roles in innate and adaptive immune responses. In this review, we provide an overview of the B cell subsets present in peripheral blood of healthy individuals. A specific gating strategy is also described to clearly and univocally identify B cell subsets based on the their phenotypic traits by flow cytometric analysis.

scTAM-seq enables targeted high-confidence analysis of DNA methylation in single cells

“…2A-D). The gradual gain of CD27 appeared to be associated with gradual progression of cellular differentiation, with CD27-negative ns-memory B cells representing early, atypical memory B cells that are present at the highest frequencies at birth [20, 21]. Furthermore, linking the different DNA methylation patterns within the ns-memory B cell cluster to chromatin states [22] showed that one CpG cluster that loses methylation (CpG cluster 1) is enriched for heterochromatin (Fisher’s test p-value: 2.28×10 −9 ), while gain of methylation (CpG cluster 5) occurs more frequently in polycomb-associated poised promoters (Fisher’s test p-value: 2.7×10 −4 ) (Fig.…”

scTAM-seq enables targeted high-confidence analysis of DNA methylation in single cells

“…2a-d). The gradual gain of CD27 appeared to be associated with gradual progression of cellular differentiation, with CD27-negative ns-memory B cells representing early, atypical memory B cells that are present at the highest frequencies at birth [22,23]. Furthermore, linking the different DNAm patterns within the ns-memory B cell cluster to chromatin states [24] showed that one CpG cluster that loses methylation along differentiation pseudotime (CpG cluster 1) is enriched for heterochromatin (Fisher's test p-value: 2.28×10 −9 ), while gain of methylation (CpG cluster 5) occurs more frequently in polycomb-associated poised promoters (Fisher's test p-value: 2.7×10 −4 ) (Fig.…”

Comprehensive phenotyping of human peripheral blood B lymphocytes in pathological conditions

“…Live cells are identified based on the FSC/SSC lympho‐monocyte gate and then selected as CD45 pos CD19 pos B cells [ 6 ]. Briefly, we first discriminate transitional B cells (CD24 pos CD38 bright ) and plasmablasts (CD24 neg CD38 bright ).…”