Comprehensive Proteomic Analysis of Colon Cancer Tissue Revealed the Reason for the Worse Prognosis of Right-Sided Colon Cancer and Mucinous Colon Cancer at the Protein Level

Abstract

To clarify the molecular mechanisms underlying the poor prognosis of right-sided and mucinous colon cancer at the proteomic level. A tandem mass tag-proteomics approach was used to identify differentially expressed proteins (DEPs) in colon carcinoma tissues from different locations and with different histological types to reveal the underlying mechanisms of these differences at the protein level. In additional, the DEPs were analyzed using bioinformatics methods. The proteomics profiles among colon cancers with different tumor locations and histological types were dramatically distinguished. In terms of tumor locations, the right-sided carcinoma specific DEPs may promote the tumor progression via activating inflammation, metastasis associated pathways. When referring to histological types, the mucinous colon cancers perhaps increased the invasion and metastasis through distinct mechanisms in different tumor locations. For mucinous cancer located in right-sided colon, the mucinous specific DEPs were mainly associated with ECM-related remodeling and the IL-17 signal pathway. For mucinous cancer located in left-sided colon, the mucinous specific DEPs showed a strong relationship with ACE2/Ang-(1–7)/MasR axis. The proteomics profiles of colon cancers showed distinct differences related to locations and histological types. These results suggested a distinct mechanism underlying the diverse subtypes of colon cancers.

CRISPR-Cas9 screening identifies INTS3 as an anti-apoptotic RNA-binding protein and therapeutic target for colorectal cancer

“…STRING analysis further indicated candidate genes were clustered into three pathways: tRNA aminoacylation for protein translation and mitochondrial translation, mRNA splicing and translation ( Figure 1 E). Several RBP candidates which have been identified as oncogenes in CRC were identified and ranked high in our screening, including EIF6, 16 FARSA, 17 RRP12 18 and EFL1 19 ( Figure 1 E). Then, we explored The Cancer Genome Atlas (TCGA) database and analyzed by The University of ALabama at Birmingham CANcer data analysis Portal (UALCAN) data analysis portal and found that 85% of RBP candidates are highly expressed in CRC tumors ( Figure S1 C).…”

Clinical Significance and Potential Molecular Mechanisms of Angiotensin-Converting Enzyme 2 in Colorectal Cancer

“…This downregulation of the ACE2/Ang-(1-7)/Mas signaling axis may contribute to tumor progression and metastasis. 44 This regulatory axis provides a partial explanation for the low expression of ACE2 in colonic mucinous adenocarcinoma.…”

The impacts of sarcopenia on outcomes of patients with left-sided colon or rectal cancer after curative surgery

“…These previous studies included not so many patients. Additionally, it has been reported that right-sided colon cancer was signi cant different from left-sided colon and rectal cancer in terms of long-term prognosis and cancer biology [13][14][15]. However, as far as we know, studies investigating the prognostic signi cance of sarcopenia among patients with left-sided colon or rectal cancer remain scarce.…”