Comprehensive proteomic analysis of exoproteins expressed by ERIC I, II, III and IV<i>Paenibacillus larvae</i>genotypes reveals a wide range of virulence factors

Erban, Tomáš; Zítek, Justyna; Bodrinova, Miroslava; Talacko, Pavel; Bartoš, Milan; Hrabák, Jaroslav

doi:10.1080/21505594.2019.1603133

Cited by 16 publications

(57 citation statements)

References 89 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Four genotypes of P. larvae have been established using by mass spectrometry [ 39 ] and repetitive-element PCR analysis [ 40 , 41 ]. Primers specific to enterobacterial repetitive intergenic consensus (ERIC) sequences revealed four distinct banding patterns, resulting in the nomenclature of P. larvae ERIC I–IV.…”

Section: Introductionmentioning

confidence: 99%

The N-terminus ofPaenibacillus larvaeC3larvinA modulates catalytic efficiency

2021

View full text Add to dashboard Cite

C3larvinA was recently described as a mono-ADP-ribosyltransferase (mART) toxin from the enterobacterial repetitive intergenic consensus (ERIC) III genotype of the agricultural pathogen, Paenibacillus larvae. It was shown to be the full-length, functional version of the previously described C3larvintrunc toxin, due to a 33-residue extension of the N-terminus of the protein. In the present study, a series of deletions and substitutions were made to the N-terminus of C3larvinA to assess the contribution of the α1-helix to toxin structure and function. Catalytic characterization of these variants identified Asp23 and Ala31 residues as supportive to enzymatic function. A third residue, Lys36, was also found to contribute to the catalytic activity of the enzyme. Analysis of the C3larvinA homology model revealed that these three residues were participating in a series of interactions to properly orient both the Q-X-E and S-T-S motifs. Ala31 and Lys36 were found to associate with a structural network of residues previously identified in silico, whereas Asp23 forms novel interactions not previously described. At last, the membrane translocation activity into host target cells of each variant was assessed, highlighting a possible relationship between protein dipole and target cell entry.

show abstract

Section: Introductionmentioning

confidence: 99%

The N-terminus ofPaenibacillus larvaeC3larvinA modulates catalytic efficiency

2021

View full text Add to dashboard Cite

show abstract

“…Epsilon toxins change the cell permeability for ions by forming large membrane pores, causing cell death and edema in animal models [67]. It was recently suggested that a protein of the ETX/MT2 family is an important virulence factor in two subtypes of P. larvae, the causative pathogen of American foulbrood [68]. It is thus plausible that melissotoxin A increases the virulence of M. plutonius by corrupting the cells of the larva's digestive tract.…”

Section: Role Of Melissotoxin a In Virulencementioning

confidence: 99%

Putative determinants of virulence inMelissococcus plutonius, the bacterial agent causing European foulbrood in honey bees

et al. 2020

View full text Add to dashboard Cite

Melissococcus plutonius is a bacterial pathogen that causes epidemic outbreaks of European foulbrood (EFB) in honey bee populations. The pathogenicity of a bacterium depends on its virulence, and understanding the mechanisms influencing virulence may allow for improved disease control and containment. Using a standardized in vitro assay, we demonstrate that virulence varies greatly among sixteen M. plutonius isolates from five European countries. Additionally, we explore the causes of this variation. In this study, virulence was independent of the multilocus sequence type of the tested pathogen, and was not affected by experimental coinfection with Paenibacillus alvei, a bacterium often associated with EFB outbreaks. Virulence in vitro was correlated with the growth dynamics of M. plutonius isolates in artificial medium, and with the presence of a plasmid carrying a gene coding for the putative toxin melissotoxin A. Our results suggest that some M. plutonius strains showed an increased virulence due to the acquisition of a toxin-carrying mobile genetic element. We discuss whether strains with increased virulence play a role in recent EFB outbreaks.

show abstract

“…Bacteria from this genus were also shown to produce a plethora of enzymes, like amylases, cellulases, hemicellulases, lipases, pectinases, oxygenases or dehydrogenases ( Grady et al, 2016 ). The proteome of P. larvae includes a wide range of virulence factors including proteases and toxins ( Erban et al, 2019 ). Paenibacillus validus stimulated the growth of Glomus intraradices ( Hildebrandt et al, 2006 ) and P. vortex facilitates dispersal of Aspergillus fumigatus ( Ingham et al, 2011 ).…”

Section: Discussionmentioning

confidence: 99%

Metabolic Potential, Ecology and Presence of Associated Bacteria Is Reflected in Genomic Diversity of Mucoromycotina

et al. 2021

View full text Add to dashboard Cite

Mucoromycotina are often considered mainly in pathogenic context but their biology remains understudied. We describe the genomes of six Mucoromycotina fungi representing distant saprotrophic lineages within the subphylum (i.e., Umbelopsidales and Mucorales). We selected two Umbelopsis isolates from soil (i.e., U. isabellina, U. vinacea), two soil-derived Mucor isolates (i.e., M. circinatus, M. plumbeus), and two Mucorales representatives with extended proteolytic activity (i.e., Thamnidium elegans and Mucor saturninus). We complement computational genome annotation with experimental characteristics of their digestive capabilities, cell wall carbohydrate composition, and extensive total lipid profiles. These traits inferred from genome composition, e.g., in terms of identified encoded enzymes, are in accordance with experimental results. Finally, we link the presence of associated bacteria with observed characteristics. Thamnidium elegans genome harbors an additional, complete genome of an associated bacterium classified to Paenibacillus sp. This fungus displays multiple altered traits compared to the remaining isolates, regardless of their evolutionary distance. For instance, it has expanded carbon assimilation capabilities, e.g., efficiently degrades carboxylic acids, and has a higher diacylglycerol:triacylglycerol ratio and skewed phospholipid composition which suggests a more rigid cellular membrane. The bacterium can complement the host enzymatic capabilities, alter the fungal metabolism, cell membrane composition but does not change the composition of the cell wall of the fungus. Comparison of early-diverging Umbelopsidales with evolutionary younger Mucorales points at several subtle differences particularly in their carbon source preferences and encoded carbohydrate repertoire. Nevertheless, all tested Mucoromycotina share features including the ability to produce 18:3 gamma-linoleic acid, use TAG as the storage lipid and have fucose as a cell wall component.

show abstract

Comprehensive proteomic analysis of exoproteins expressed by ERIC I, II, III and IVPaenibacillus larvaegenotypes reveals a wide range of virulence factors

Cited by 16 publications

References 89 publications

The N-terminus ofPaenibacillus larvaeC3larvinA modulates catalytic efficiency

The N-terminus ofPaenibacillus larvaeC3larvinA modulates catalytic efficiency

Putative determinants of virulence inMelissococcus plutonius, the bacterial agent causing European foulbrood in honey bees

Metabolic Potential, Ecology and Presence of Associated Bacteria Is Reflected in Genomic Diversity of Mucoromycotina

Contact Info

Product

Resources

About