Comprehensive quantification of flavonoids and salicylic acid representative of Salix spp. using microLiquid Chromatography-Triple Quadrupole Mass Spectrometry: the importance of drying procedures and extraction solvent when performing classical solid-liquid extraction

Curtasu, Mihai Victor; Nørskov, Natalja P.

doi:10.1016/j.chroma.2023.464139

Cited by 6 publications

(1 citation statement)

References 40 publications

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“…The concentrations of CAT, EPIC, QUE, RUT, GAL and SALA after 48 h of incubation were measured using a triple quadrupole mass spectrometer connected to a microLC according to the microLC-MS/MS method 32 and adjusted to rumen fluid. The following standards were purchased from Sigma-Aldrich (Merck KGaA, Darmstadt, Germany), chemical purity (CP) and CAS number listed below: catechin ≥ 98% (18829-70-4), epicatechin ≥ 98% (490-46-0), quercetin ≥ 95% (117-39-5), rutin ≥ 94% (207671-50-9) and salicylic acid 99% (69-72-7) and labelled standards catechin-2,3,4- 13 C 3 99 atom % 13 C (98% CP), salicylic acid-D 4 certified reference material (78646-17-0) and enterolactone-2,3,5- 13 C3 (918502-72-4) from Toronto Research Chemicals (Toronto, ON, Canada).…”

Section: Methodsmentioning

confidence: 99%

Methane reduction by quercetin, tannic and salicylic acids: influence of molecular structures on methane formation and fermentation in vitro

Nørskov,

Battelli,

Curtasu

et al. 2023

Sci Rep

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Plant secondary metabolites (PSMs) can potentially reduce ruminal methane formation. However, related to differences in their molecular structures, it is not yet clear what causes an anti-methanogenic effect. In an in vitro system simulating rumen fermentation, we investigated the impact of eight compounds with distinct chemical characteristics (gallic and salicylic acids, tannic acid, catechin, epicatechin, quercetin, rutin, and salicin) when added to a basal feed (maize silage) at a concentration of 12% of the feed dry matter. After 48 h of incubation in buffered rumen fluid, methane production was significantly lowered by quercetin (43%), tannic acid (39%) and salicylic acid (34%) compared to the control (maize silage alone) and without changes in total volatile fatty acid production during fermentation. No other PSM reduced methane formation as compared to control but induced significant differences on total volatile fatty acid production. The observed differences were related to lipophilicity, the presence of double bond and carbonyl group, sugar moieties, and polymerization of the compounds. Our results indicate the importance of distinct molecular structures of PSMs and chemical characteristics for methane lowering properties and volatile fatty acid formation. Further systematic screening studies to establish the structure–function relationship between PSMs and methane reduction are warranted.

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Section: Methodsmentioning

confidence: 99%

Methane reduction by quercetin, tannic and salicylic acids: influence of molecular structures on methane formation and fermentation in vitro

Nørskov,

Battelli,

Curtasu

et al. 2023

Sci Rep

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Effect of different drying methods on the nutritional composition and phenolic compounds of the brown macroalga, Fucus vesiculosus (Fucales, Phaeophyceae)

Dhakal,

Pandey,

van der Heide

et al. 2024

J Appl Phycol

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The application of macroalgae for food and feed has been increasing continuously due to their nutritional and health-promoting properties. Efficient post-harvest drying is needed to remove moisture content from macroalgal biomass without negatively affecting its nutritional value. We hypothesized that low-temperature oven drying would preserve most of the nutrients and polyphenols in macroalgae. The polyphenol-rich brown macroalga, Fucus vesiculosus, was exposed to one of the following treatments: freeze-drying (FD; for 72 h), oven drying at 40 ℃ (OD40; for 24 h), and oven drying at 80 ℃ (OD80; for 24 h). The concentration of total fatty acids and the sum of saturated, mono-, and polyunsaturated fatty acids exhibited a decreasing trend with higher drying temperatures (FD > OD40 > OD80), indicating the sensitivity of fatty acids to a high drying temperature. However, the sum of total or essential amino acids was significantly higher (p < 0.05) in OD80 compared to OD40 and FD biomass. In this study, the average N-protein-conversion factor for dried F. vesiculosus remained relatively stable (~ 4.64) across drying treatments. The total polyphenol content remained unaffected by the drying treatment, although it tended to decrease with increasing drying temperature. Targeted metabolomics revealed three classes of phenolic compounds: phenylpropanoids, flavones, and flavonols. A low-temperature oven drying appears to be a suitable method to preserve nutrients and polyphenols in brown macroalgae. Future studies are needed to evaluate the impact of drying methods on other bioactive compounds and to understand the economic sustainability of oven drying.

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Quantitative distribution of flavan-3-ols, procyanidins, flavonols, flavanone and salicylic acid in five varieties of organic winter dormant Salix spp. by LC-MS/MS

Curtasu,

Nørskov

2024

Heliyon

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Comprehensive quantification of flavonoids and salicylic acid representative of Salix spp. using microLiquid Chromatography-Triple Quadrupole Mass Spectrometry: the importance of drying procedures and extraction solvent when performing classical solid-liquid extraction

Cited by 6 publications

References 40 publications

Methane reduction by quercetin, tannic and salicylic acids: influence of molecular structures on methane formation and fermentation in vitro

Methane reduction by quercetin, tannic and salicylic acids: influence of molecular structures on methane formation and fermentation in vitro

Effect of different drying methods on the nutritional composition and phenolic compounds of the brown macroalga, Fucus vesiculosus (Fucales, Phaeophyceae)

Quantitative distribution of flavan-3-ols, procyanidins, flavonols, flavanone and salicylic acid in five varieties of organic winter dormant Salix spp. by LC-MS/MS

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