Comprehensive review of two groups of flavonoids in Carthamus tinctorius L.

Xian, Bin; Wang, Rui; Jiang, Huajuan; Zhou, Yongfeng; Yan, Jie; Huang, Xulong; Jiang, Chen; Wu, Qinghua; Chen, Chao; Xi, Ziqing; Ren, Chaoxiang; Pei, Jin

doi:10.1016/j.biopha.2022.113462

Cited by 31 publications

(22 citation statements)

References 221 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, only kaempferol glycosides were detected in the protoplasts, and no glycosides of other flavonols, such as quercetin and isorhamnetin, were identified. It may be due to the fact that kaempferol is the most abundant flavonol in safflower, 5 and the protoplasts were prepared from fresh, unflowering corollas, while metabolomics measured blooming flowers, resulting in slight differences in their compositions. On the other hand, although CtUGT3 49 NpUGT6 has been identified in the embryos of Nelumbo nucifera and catalyzes the 3-OH glycosylation of quercetin.…”

Section: ■ Discussionmentioning

confidence: 99%

“…Various types of kaempferol glycosides are abundant in safflower, and kaempferol 3-O-glucoside (astragalin) is one of the most common glycosides. 5 Astragalin is found in many plants with various biological activities, such as antitumor, anti-inflammatory, antiallergic, antioxidant, and antibacterial activities. 6 The basic metabolic pathway of flavonoid biosynthesis is well known, particularly in model plants.…”

Section: ■ Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

“…To determine the kinetic parameters of CtUGT3 for naringenin, assays containing 50 mM Na 2 HPO 4 −NaH 2 PO 4 (pH 8.0), 1 μM CtUGT3, saturating UDP-glucose, and varying concentrations (5,10,20,40,80,100,200, 400, 800, 1000, and 1500 μM) of naringenin were performed at 37 °C for 5 min with a final volume of 100 μL. To determine the kinetic parameters of CtUGT3 for apigenin, assays containing 50 mM Na 2 HPO 4 −NaH 2 PO 4 (pH 8.0), 1 μM CtUGT3, saturating UDP-glucose, and varying concentrations (5,10,20,40,80,100,200, 400, 800, 1000, and 1500 μM) of apigenin were performed at 37 °C for 5 min with a final volume of 100 μL. To determine the kinetic parameters of CtUGT3 for kaempferol, assays containing 50 mM Na 2 HPO 4 −NaH 2 PO 4 (pH 8.0), 1 μM CtUGT3, saturating UDP-glucose, and varying concentrations (100, 200, 400, 800, 1000, 1200, 1600, 1800, 2000, and 2400 μM) of kaempferol, were performed at 37 °C for 5 min with a final volume of 100 μL.…”

Section: ■ Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Identification and Characterization of CtUGT3 as the Key Player of Astragalin Biosynthesis in Carthamus tinctorius L.

Ren,

Xi,

Xian

et al. 2023

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

Safflower (Carthamus tinctorius L.) is a multipurpose economic crop that is distributed worldwide. Flavonoid glycosides are the main bioactive components in safflower, but only a few UDP-glycosyltransferases (UGT) have been identified. Three differentially expressed UGT genes related with the accumulation of 9 flavonoid O-glycosides were screened from metabolomics and transcriptome analysis. Safflower corolla protoplasts were used to confirm the glycosylation ability of UGT candidates in vivo for the first time. The astragalin content was significantly increased only when CtUGT3 was overexpressed. CtUGT3 also showed flavonoid 3-OH and 7-OH glycosylation activities in vitro. Molecular modeling and site-directed mutagenesis revealed that G15, T136, S276, and E384 were critical catalytic residues for the glycosylation ability of CtUGT3. These results demonstrate that CtUGT3 has a flavonoid 3-OH glycosylation function and is involved in the biosynthesis of astragalin in safflower. This study provides a reference for flavonoid biosynthesis genes research in nonmodel plants.

show abstract

Section: ■ Discussionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

Section: ■ Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Identification and Characterization of CtUGT3 as the Key Player of Astragalin Biosynthesis in Carthamus tinctorius L.

Ren,

Xi,

Xian

et al. 2023

J. Agric. Food Chem.

Self Cite

View full text Add to dashboard Cite

show abstract

“…In light of these facts, Sa ower (Carthamus tinctorius L.) seed can act as the most abundant terrestrial source of PUFAs, which is a great replacement for the poultry industry. Sa ower oil was known as the "king of linoleic acid" due to its high linoleic acid (80%) content (Xian et al 2022). In the human body, linoleic acid is classi ed as an essential fatty acid, which belongs to the unsaturated group of fats, lowers cholesterol and prevents heart disease (Bruen et al 2017).…”

Section: )mentioning

confidence: 99%

Growth performance and hematological parameters of broiler chickens fed gradually increasing levels of supplemental safflower seed

Rathaur

Chandra

Prakash

2023

Preprint

View full text Add to dashboard Cite

Fats and oils are used to enhance the palatability of feed, absorb fat-soluble vitamins, and pass digesta through the gastrointestinal tract of poultry. The goal of current study was to see how dietary supplementation of Safflower seed (SS) affected on the production and hematological parameters of broiler chickens. A total of 200 male chickens (7-day-old) were randomly distributed into 5 treatments with each 5 replicate pens of 8 chicks in a 42-day trial. Each group was randomly allocated to one of 5 dietary treatments: (0, 2.5, 5, 7.5, and 10%) SS, respectively. The experimental diets were formulated for starter (7 to 21 days) and finisher (22 to 42 days) phase. Result indicated that inclusion of SS in diet improved body weight and feed conversion ratio during 7 to 42 days (P < 0.05). The highest and lowest body weight were observed in 5% SS and 0% SS levels, respectively. The obtained results demonstrated a significantly (P < 0.01) increased content of RBC, HB, PCV, Lymphocytes, MCV, MCH, MCHC, and Platelets in the blood of broiler chickens fed with various levels of SS for five weeks. Findings suggest that, SS may use as natural energy source to increase growth performance and improve hematological parameters.

show abstract

Investigation of antibacterial and anticancer activities of biosynthesized metal‐doped and undoped zinc oxide nanoparticles

Şendal,

Üstün Özgür,

Ortadoğulu Sucu

et al. 2024

Biotech and App Biochem

View full text Add to dashboard Cite

Over the past 10 years, nanotechnology has emerged as a very promising technique for a wide range of biomedical applications. Green synthesized metal and metal oxide nanoparticles (NPs) are cheap, easy to produce in large quantities, and safe for the environment. Currently, efforts are being made to dope ZnO in order to improve its optical, electrical, and ferromagnetic qualities as well as its crystallographic quality. Actually, doping is one of the simplest methods for enhancing an NP's physicochemical characteristics because it involves introducing impure ions into the crystal lattice of the particle. In this study, the biosynthesis of zinc oxide NPs (ZnONPs) and metal‐doped (Mg2+ and Ag+) ZnONPs was carried out by using aqueous and water‐alcoholic extracts of Cynara scolymus L. leaves, Carthamus tinctorius L. flowers, and Rheum ribes L. (RrL) plant, which are rich in phytochemical content. Plant extracts act as a natural reducing, capping, and stabilizing agent in the production. The produced NPs were characterized using a variety of methods, such as ultraviolet‐visible spectroscopy, Fourier transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS), and scanning electron microscopy (SEM). The produced metal‐doped and undoped ZnONPs exhibited characteristic absorption peaks between 365 and 383 nm due to their surface plasmon resonance bands. SEM analysis revealed that the NPs were oval, nearly spherical, and spherical. In the FTIR spectra, the Zn–O bonding peak ranges from 400 to 700 cm−1. The peaks obtained in the range of 407–562 cm−1 clearly represent the Zn–O bond. In addition, the FTIR results showed that there were notable amounts of phenol and flavonoid compounds in both the prepared extract and ZnONPs. According to DLS analysis results, the size distribution of produced NPs is between 120 and 786 nm. The antibacterial properties of green produced NPs on Gram‐positive (Staphylococcus aureus RN4220) and Gram‐negative (Escherichia coli DH10B) bacterial strains were investigated by agar well diffusion method. In studies investigating the anticancer activities of biosynthesized NPs, mouse fibroblast cells (L929) were used as healthy cells and human cervical cancer cells (HeLa) were used as cancer cells. Only the produced Ag‐ZnONPs showed potent dose‐dependent antibacterial activity (at concentrations higher than 100 µg/mL) against Gram‐positive and Gram‐negative bacteria. RrL‐ZnONP‐600 and RrL‐ZnONP‐800 NPs produced with water–ethanol extract of RrL plant and calcined at 600 and 800°C were effective at high concentrations in healthy cells and at low concentrations in HeLa cancer cells, showing that they have the potential to be anticancer agents. The study's findings highlight the potential of green synthesis techniques in the production of medicinal nanomaterials for the treatment of cancer and other biological uses.

show abstract

Comprehensive review of two groups of flavonoids in Carthamus tinctorius L.

Cited by 31 publications

References 221 publications

Identification and Characterization of CtUGT3 as the Key Player of Astragalin Biosynthesis in Carthamus tinctorius L.

Identification and Characterization of CtUGT3 as the Key Player of Astragalin Biosynthesis in Carthamus tinctorius L.

Growth performance and hematological parameters of broiler chickens fed gradually increasing levels of supplemental safflower seed

Investigation of antibacterial and anticancer activities of biosynthesized metal‐doped and undoped zinc oxide nanoparticles

Contact Info

Product

Resources

About