Comprehensive screening and identification of natural inducible nitric oxide synthase inhibitors from Radix Ophiopogonis by off-line multi-hyphenated analyses

Guo, Yanju; Fu, Rao; Yin, Qian; Zhou, Zhenzhen; Liu, Haichun; Qi, Jin; Zhang, Boli; Yu, Boyang

doi:10.1016/j.chroma.2019.01.029

Cited by 20 publications

(9 citation statements)

References 38 publications

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“…Homoisoflavonoids, a special type of flavonoids with B‐ring and C‐ring being connected by an additional CH 2 group, are rare in plants, and less than 300 homoisoflavonoids have been isolated and identified in nature products [1]. Recently, homoisoflavonoids have sparked interests due to their various biological activities, for example, antioxidant [2], anti‐adipogenic [3], cytotoxic [4], anti‐inflammatory [5], and anti‐diabetic activities [6]. Hence, it is significant from the viewpoint of health‐promoting functionalities to exploit plants, fruits, or vegetables rich in homoisoflavonoids for the development of functional foods, nutraceuticals, or medicines.…”

Section: Introductionmentioning

confidence: 99%

“…Roots of O. japonicas are considered as an effective clinical tonic and strengthening drug for treatment of cough, arrhythmias, inflammation, respiratory disease, thrombosis, and gastrointestinal disorders [7]. Previous chemical studies have found that O. japonicus contains homoisoflavonoids, steroidal saponins, polysaccharides, and organic acids [2,5,7]. It was reported that about 50 homoisoflavoniods were purified and identified by repeated column fractionations [1].…”

Section: Introductionmentioning

confidence: 99%

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Homoisoflavonoids profiling of Ophiopogon japonicus by off‐line coupling high‐speed countercurrent chromatography with high‐performance liquid chromatography–diode array detector‒quadrupole time‐of‐flight tandem mass spectrometry

Deng

Tong

et al. 2020

J of Separation Science

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Roots of Ophiopogon japonicus have been used as a functional food ingredient and traditional Chinese medicine for a long time in China. Homoisoflavonoids are one of the major kinds of bioactive compounds in O. japonicus; however, literature data about its homoisoflavonoids profile are scarce because of the complex ingredients with low abundance. Here, homoisoflavonoid fraction was prepared by petroleum ether extraction. Then, a high-speed countercurrent chromatography offline coupling with high-performance liquid chromatography-diode array detectorquadrupole time-of-flight tandem mass spectrometry was developed for systematic identification of homoisoflavonoids. After that, 39 homoisoflavonoids, including 29 homoisoflavanone and 10 homoisoflavone, were unambiguously or tentatively identified, while 12 of them were reported in O. japonicus for the first time. Finally, eight available homoisoflavonoids were sensitively, precisely, and accurately determined by standard calibration curves, with limit of detection and limit of quantification in the range of 0.05-0.30 μg/mL and 0.12-0.66 μg/mL, relative standard deviation less than 7.3% for intra-and interday variations, and recovery at 94.5-105.2%. Collectively, our developed method is efficient, reliable, and valuable to profile chemical components of complex natural products.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Homoisoflavonoids profiling of Ophiopogon japonicus by off‐line coupling high‐speed countercurrent chromatography with high‐performance liquid chromatography–diode array detector‒quadrupole time‐of‐flight tandem mass spectrometry

Deng

Tong

et al. 2020

J of Separation Science

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“…This work proved principal component analysis (PCA) is an efficient tool for optimize UF conditions. In addition, Guo et al (2019) adopted the denatured enzyme method to screen inducible nitric oxide synthase (iNOS) inhibitors from Ophiopogon japonicus . Seven specific compounds were unambiguously identified.…”

Section: Ultrafiltraion‐based Ba‐msmentioning

confidence: 99%

Label‐free Bio‐affinity Mass Spectrometry for Screening and Locating Bioactive Molecules

Tao

Yan

Cai

2019

Mass Spectrometry Reviews

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Despite the recent increase in the development of bioactive molecules in the drug industry, the enormous chemical space and lack of productivity are still important issues. Additional alternative approaches to screen and locate bioactive molecules are urgently needed. Label‐free bio‐affinity mass spectrometry (BA‐MS) provides opportunities for the discovery and development of innovative drugs. This review provides a comprehensive portrayal of BA‐MS techniques and of their applications in screening and locating bioactive molecules. After introducing the basic principles, alongside some application notes, the current state‐of‐the‐art of BA‐MS‐assisted drug discovery is discussed, including native MS, size‐exclusion chromatography‐MS, ultrafiltration‐MS, solid‐phase micro‐extraction‐MS, and cell membrane chromatography‐MS. Finally, several challenges and limitations of the current methods are summarized, with a view to potential future directions for BA‐MS‐assisted drug discovery. © 2019 John Wiley & Sons Ltd. Mass Spec Rev

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“…Additionally, in recent years, ultrafiltration coupled to liquid chromatography-mass spectrometry has been proven useful as screening assay and powerful tool to reduce time and cost for the identification of naturally occurring enzyme inhibitors [ 17 , 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Identification of Asiaticoside from Centella erecta (Apiaceae) as Potential Apyrase Inhibitor by UF-UHPLC-MS and Its In Vivo Antischistosomal Activity

et al. 2022

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Schistosomiasis, caused by parasites of the genus Schistosoma, is a neglected disease with high global prevalence, affecting more than 240 million people in several countries. Praziquantel (PZQ) is the only drug currently available for the treatment. S. mansoni NTPDases (known as SmNTPDases, ATP diphosphohydrolases or ecto-apyrases) are potential drug targets for the discovery of new antischistosomal drugs. In this study, we screen NTPDases inhibitors from Centella erecta (Apiaceae) using an ultrafiltration combined UHPLC-QTOF-MS method and potato apyrase, identifying asiaticoside as one of the apyrase-binding compounds. After isolation of asiaticoside from C. erecta extract, we assessed its in vivo antischistosomal activities against Schistosoma mansoni worms and its in vitro enzymatic apyrase inhibition. Also, molecular docking analysis of asiaticoside against potato apyrase, S. mansoni NTPDases 1 and 2 were performed. Asiaticoside showed a significant in vitro apyrase inhibition and molecular docking studies corroborate with its possible actions in potato apyrase and S. mansoni NTPDases. In mice harboring a patent S. mansoni infection, a single oral dose of asiaticoside (400 mg/kg. p.o.) showed significantly in vivo antischistosomal efficacy, markedly decreasing the total worm load and egg burden, giving support for further exploration of apyrase inhibitors as antischistosomal agents.

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Comprehensive screening and identification of natural inducible nitric oxide synthase inhibitors from Radix Ophiopogonis by off-line multi-hyphenated analyses

Cited by 20 publications

References 38 publications

Homoisoflavonoids profiling of Ophiopogon japonicus by off‐line coupling high‐speed countercurrent chromatography with high‐performance liquid chromatography–diode array detector‒quadrupole time‐of‐flight tandem mass spectrometry

Homoisoflavonoids profiling of Ophiopogon japonicus by off‐line coupling high‐speed countercurrent chromatography with high‐performance liquid chromatography–diode array detector‒quadrupole time‐of‐flight tandem mass spectrometry

Label‐free Bio‐affinity Mass Spectrometry for Screening and Locating Bioactive Molecules

Identification of Asiaticoside from Centella erecta (Apiaceae) as Potential Apyrase Inhibitor by UF-UHPLC-MS and Its In Vivo Antischistosomal Activity

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