Comprehensive screening and separation of cyclooxygenase-2 inhibitors from Pterocephalus hookeri by affinity solid-phase extraction coupled with preparative high-performance liquid chromatography

Zhu, Yunhe; Wang, Weidong; Liu, Zenggen; Jiang, S. Y.; Jiang, Lei; Mei, Lijuan

doi:10.1016/j.jchromb.2021.122981

Cited by 7 publications

(8 citation statements)

References 35 publications

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“…The affinity solid-phase extraction HPLC method for screening and analyzing COX-2 inhibitors is depicted in Figure 2 . The ASPE column packed with silica gel which functionalized with COX-2 by the glutaraldehyde cross-linking method is critical to this strategy [ 15 ]. Experimental details of the ASPE column fabrication referenced from the literature with slight modifications [ 25 ].…”

Section: Resultsmentioning

confidence: 99%

“…The conversions of AA to prostaglandin G 2 and prostaglandin G 2 to prostaglandin H 2 were catalyzed by COX-2 enzyme [ 15 ]. In order to verify the screening result, sylvestroside I was tested its COX-2 inhibitory activity on the LPS-activated RAW 264.7 macrophage cells.…”

Section: Resultsmentioning

confidence: 99%

“…At the same time, studies have shown that iridoids were the main material basis for P. hookeri to exert the above medicinal effects [ 14 ]. In addition, it also includes triterpenes [ 4 ], phenylpropanoids [ 3 ], flavonoids [ 15 ], etc. However, a vast amountof research on the activities of P. hookeri are based on the crude extracts, and only a few studies have analyzed the activity of individual compounds [ 5 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

“…Then, preparation can substantially purify the target compound with a purity of >95% [ 32 ]. The medium-pressure chromatographic tower is based on the absorption chromatography with the advantage of higher sample loading, the convenience of packing procedure, lower cost, and online detection and has widely used for crude extracts purification and natural compounds enrichment [ 15 , 28 , 33 ]. Therefore, it is necessary to isolate the potential COX-2 inhibitors by pretreatment of medium-pressure chromatographic tower and suitable preparative HPLC strategy for chemical structural characterization and inhibitory activity verification.…”

Section: Introductionmentioning

confidence: 99%

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Targeted Separation of COX-2 Inhibitor from Pterocephalus hookeri Using Preparative High-Performance Liquid Chromatography Directed by the Affinity Solid-Phase Extraction HPLC System

et al. 2021

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Pterocephalus hookeri, as a kind of popular traditional Tibetan medicine, is reputed to treat inflammatory related diseases. In the present work, a cyclooxygenase-2 functionalized affinity solid-phase extraction HPLC system was developed and combined with preparative-HPLC for rapidly screening and separating cyclooxygenase-2 ligand from P. hookeri extracts. Firstly, ligands of cyclooxygenase-2 were screened from extracts by affinity solid-phase extraction HPLC system. Then directed by the screening results, the recognized potential active compounds were targeted separated. As a result, the major cyclooxygenase-2 inhibitor of P. hookeri was obtained with a purity of >95%, which was identified as sylvestroside I. To test the accuracy of this method, the anti-inflammatory activity of sylvestroside I was inspected in lipopolysaccharide-induced RAW 264.7 cells. The results show that sylvestroside I significantly suppressed the release of prostaglandin E2 with dose-dependent, which was in good agreement with the screening result of the affinity solid-phase method. This method of integration of screening and targeted separation proved to be very efficient for the recognition and isolation of cyclooxygenase-2 inhibitors from natural products.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Targeted Separation of COX-2 Inhibitor from Pterocephalus hookeri Using Preparative High-Performance Liquid Chromatography Directed by the Affinity Solid-Phase Extraction HPLC System

et al. 2021

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“…Recently, numerous methods have been developed for the rapid screening of enzyme inhibitors such as ultrafiltration, bioaffinity chromatography, membrane affinity chromatography, and ligand fishing. , Among them, ligand fishing using target protein-immobilized magnetic nanoparticles as adsorbents combined with high-performance liquid chromatography-mass spectrometry (HPLC-MS) has been recognized as one of the most powerful strategies for discovering target ingredients from natural products. , Our group has immobilized MAO-B onto magnetic nanoparticles for screening of the inhibitors in three traditional Chinese medicinal plants, Cortex fraxini, Pericarpium granati, and Lonicera japonica. , …”

Section: Introductionmentioning

confidence: 99%

Screening of Monoamine Oxidase B Inhibitors from Fragaria nubicola by Ligand Fishing and Their Neuroprotective Effects

Liu

Bai

et al. 2022

J. Agric. Food Chem.

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Fragaria nubicola, known as Tibetan strawberry, is an edible plant possessing various health-promoting effects. However, its functional compositions were rarely studied. In this work, monoamine oxidase B (MAO-B) inhibitors in this plant were rapidly screened using the enzyme-functionalized magnetic nanoparticles coupled with UPLC-QTOF-MS. Two inhibitors, quercetin-3-O-β-D-glucuronide-6″-methyl ester (1) and kaempferol-3-O-β-D-glucuronide-6″-methyl ester (2), were identified from this plant with the IC 50 values of 19.44 ± 1.17 and 22.63 ± 1.78 μM, respectively. Enzyme kinetic analysis and molecular docking were carried out to investigate the mechanism of inhibition. Contents of both compounds as well as those of total phenolics and flavonoids were quantified to be 24.76

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Ligand fishing as a tool to screen natural products with anticancer potential

Filho

Oliveira

Leal

et al. 2023

J of Separation Science

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Cancer is the second leading cause of death in the world and its incidence is expected to increase with the aging of the world's population and globalization of risk factors. Natural products and their derivatives have provided a significant number of approved anticancer drugs and the development of robust and selective screening assays for the identification of lead anticancer natural products are essential in the challenge of developing personalized targeted therapies tailored to the genetic and molecular characteristics of tumors. To this end, a ligand fishing assay is a remarkable tool to rapidly and rigorously screen complex matrices, such as plant extracts, for the isolation and identification of specific ligands that bind to relevant pharmacological targets. In this paper, we review the application of ligand fishing with cancer‐related targets to screen natural product extracts for the isolation and identification of selective ligands. We provide critical analysis of the system configurations, targets, and key phytochemical classes related to the field of anticancer research. Based on the data collected, ligand fishing emerges as a robust and powerful screening system for the rapid discovery of new anticancer drugs from natural resources. It is currently an underexplored strategy according to its considerable potential.

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Comprehensive screening and separation of cyclooxygenase-2 inhibitors from Pterocephalus hookeri by affinity solid-phase extraction coupled with preparative high-performance liquid chromatography

Cited by 7 publications

References 35 publications

Targeted Separation of COX-2 Inhibitor from Pterocephalus hookeri Using Preparative High-Performance Liquid Chromatography Directed by the Affinity Solid-Phase Extraction HPLC System

Targeted Separation of COX-2 Inhibitor from Pterocephalus hookeri Using Preparative High-Performance Liquid Chromatography Directed by the Affinity Solid-Phase Extraction HPLC System

Screening of Monoamine Oxidase B Inhibitors from Fragaria nubicola by Ligand Fishing and Their Neuroprotective Effects

Ligand fishing as a tool to screen natural products with anticancer potential

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