Comprehensive screening procedure for diuretics in urine by high-performance liquid chromatography

Cooper, Sam F.; Massé, Robert; Dugal, Robert

doi:10.1016/s0378-4347(00)82884-4

Cited by 84 publications

(26 citation statements)

References 12 publications

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“…Acetonitrile-water mixtures are suitable for chromatography of neutral and weak acid diuretics [4], but for chromatography of basic and acidic compounds control of the pH of the eluent is needed, for example by adding a phosphate buffer at pH 3 [4,6,8]. Moreover, good peak symmetry of the basic diuretics is only obtained if an organic competing base [4,5,7] or an ammonium salt [3] is added to the acidic eluent. For that reasons we chose initially in our study an ODS stationary phase and a mobile phase consisting of acetonitrile and a 0.1 mol L 1 ammonium acetate solution, while the pH of the aqueous-organic eluent was adjusted to 3 with concentrated phosphoric acid.…”

Section: Resultsmentioning

confidence: 99%

Prediction of the chromatographic behaviour for a series of diuretic compounds

2000

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SummaryThe proportion of organic modifier and the pH of the acetonitrile-water mixtures used as mobile phases were optimized in order to separate a group of diuretic compounds covering a wide range of physyco-chemical properties. The Linear Solvation Energy Relationship (LSER) formalism based either on the multiparameter re*,/3 and ce scales or the single solvent polarity parameter E7 N, have been used to predict their chromatographic behaviour as a function of the percentage of acetonitrile in the eluent. Moreover, correlation established between retention and pH of the aqueous-organic mobile phases have been used to predict the chromatographic behaviour of the diuretic compounds studied as a function of the eluent pH. Linear correlation between the chromatographic retention and the E~ polarity parameter of mobile phases containing different percentages of organic modifier has been obtained. Based on the knowledge of the acid-base dissociation constant the relation between retention and mobile phase pH has also been linearized. These relationships allowed an important reduction of the experimental retention data needed for developing a given separation and a great improvement in chromatographic optimization schemes.

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Section: Resultsmentioning

confidence: 99%

Prediction of the chromatographic behaviour for a series of diuretic compounds

2000

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“…Until now analysis with HPLC/UV has required an extra clean-up step with lead acetate after the acidic extraction (Cooper et al, 1989;Tsai et al, 1991). Using the LC/MS method described here, the extra clean-up step could be omitted.…”

Section: )mentioning

confidence: 99%

Screening for 18 diuretics and probenecid in doping analysis by liquid chromatography–tandem mass spectrometry

Deventer

Delbeke

Roels

et al. 2002

Biomedical Chromatography

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A fast and selective liquid chromatography-tandem mass spectrometric (LC/MS/MS) method for the screening of 18 diuretics and probenecid in human urine is presented. Analyses were performed on a LCQ-Deca instrument equipped with ESI-interface using scan by scan polarity changing. All diuretics and probenecid were separated in less than 20 min after liquid-liquid extraction with ethyl acetate. The LOD for all substances was 100 ng/mL or better. The method was applied to detect diuretics after the oral administration of several drugs including hydrochlorothiazide, bumetanide, spironolactone, furosemide, amiloride, triamterene, chlortalidone and epithizide. All diuretics could be detected for periods up to 96 h after the intake of therapeutic amounts.

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“…Samples from the receiver chamber were collected at 0, 30, 60, 90, 120 min and were analysed for the radiolabelled marker molecules by liquid scintillation counting (Beckmann Coulter, Unterschleissheim, Germany) and for budesonide or spironolactone by HPLC in accordance with published methods (10,11). TEER (transepithelial electrical resistance) measurements (Millicell ERS, Millipore, Bedford, USA) at the start and at the end of each experiment were conducted.…”

Section: Drug Absorption Studiesmentioning

confidence: 99%