Comprehensive serotyping and epidemiology of human adenovirus isolated from the respiratory tract of Korean children over 17 consecutive years (1991–2007)

Abstract

Among the 53 serotypes of human adenoviruses (HAdVs), identified to date, only a limited number have been associated with human respiratory infections. The epidemiology of each of the serotypes differs depending on the location and/or time of surveillance. This study was performed to elucidate the epidemiology of HAdV respiratory infections by comprehensive serotyping of the HAdVs isolated from the respiratory tract of Korean children. HAdVs isolated from respiratory specimens of Korean children over 17 consecutive years (1991-2007) were typed by the neutralization test or molecular methods, including two-sets of multiplex PCR assays, and/or sequence analysis of the hexon gene. From January 1991 through December 2007, a total of 741 isolates were obtained from nasal aspirates of children hospitalized or requiring medical treatment in the emergency room. All isolates were type-determined successfully and 13 different serotypes were identified, which included HAdV serotypes 1-8, 11, 19, 34, 37, and 41. HAdV-3 (n = 285, 37.7%) and HAdV-7 (n = 181, 23.9%) were the predominant serotypes; HAdV-8, -11, -19, -34, -37, and -41 were not usually associated with respiratory diseases. HAdV-3 was present both during outbreaks and in sporadic cases. HAdV-7 emerged in a very large outbreak, followed by smaller outbreaks. HAdV-1, -2, -4, -5, and -6 were isolated sporadically throughout the study period. In conclusion, a total of 13 different serotypes of HAdV were detected among Korean children with respiratory tract infections. HAdV-3 and HAdV-7 were the most common serotypes, and they were associated with HAdV outbreaks.

Detection and Serotyping of Human Adenoviruses from Patients with Influenza-Like Illness in Mongolia

“…HAdV-B7, followed by HAdV-B3 and -D8, were the most commonly detected serotypes. A similar distribution of serotypes was observed in Korea (10). HAdV-B3 and -B7 are likely to be associated with serious acute respiratory infection.…”

“…For example, HAdV-B3 and -B7 were the most commonly encountered serotypes in surveillance conducted from 1991 to 2007 in Korea (10), and HAdV-C2 and -B3 were common in Japan between 2000 and 2007 (11). HAdV-C, especially HAdV-C1 and -C2, were common in Malaysia between 1999 and 2005 (12), whereas HAdV-B3 was common in Taiwan in 2004 and 2005 (13).…”

Clinical severity of respiratory adenoviral infection by serotypes in Korean children over 17 consecutive years (1991–2007)

“…HAdVs isolated from respiratory specimens were typed by the neutralization test, polymerase chain reaction, and hexon sequence-based typing techniques as previously reported. 9 1386-6532/$ -see front matter © 2010 Elsevier B.V. All rights reserved. doi:10.1016/j.jcv.2010.07.007 Table 1 Demographic data of patients with respiratory adenoviral (HAdV) infections according to each type.…”

“…[6][7][8] During a surveillance period of 1991-2007, major respiratory viruses isolated from nasopharyngeal aspirates have been collected and comprehensive typing of respiratory HAdVs was performed in our institute. 9 …”

Adenovirus Infections in Immunocompetent and Immunocompromised Patients

“…Conventional approaches to HAdV detection in affected samples, such as peripheral blood, stool, urine, BAL fluid, nasopharyngeal aspirates, or swabs, include primarily immunofluorescence staining for antigen detection and viral culture (43,97,190,191). However, due to the limited sensitivity and, in case of viral culture, rather long time to readout, these methods have largely been supplanted in routine clinical diagnostics by molecular screening approaches generally relying on PCR-based techniques (71,97,192).…”