Comprehensive transcriptional landscape of porcine cardiac and skeletal muscles reveals differences of aging

Chen, Jianning; Zou, Qin; Lv, Daojun; Wei, Yingying; Raza, Muhammad Ali; Yan, Chen; Li, Peilin; Xi, Xiaoyu; Xu, Huaming; Wen, Anxiang; Zhu, Li; Tang, Guoqing; Li, Mingzhou; Jiang, Anan; Liu, Yihui; Fu, Yuhua; Jiang, Yanzhi; Li, Xuewei

doi:10.18632/oncotarget.23290

Cited by 40 publications

(37 citation statements)

References 77 publications

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“…In pigs, recent work has demonstrated circRNAs' involvement in various organismal processes. For example, through a comprehensive analysis of porcine cardiac and skeletal muscles, Chen et al (2018) showed that circRNAs contribute to differences in aging. Moreover, circRNAs were defined as a new biomarker in metabolism-related diseases based on a study of circRNAs occurring in the subcutaneous adipose tissues of two pig breeds (Li et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Pituitary-Derived Circular RNAs Expression and Regulatory Network Prediction During the Onset of Puberty in Landrace × Yorkshire Crossbred Pigs

et al. 2020

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Being the center of the hypothalamus-pituitary-ovary (HPO) axis, the pituitary plays a key role in the onset of puberty. Recent studies show that circular RNAs (circRNAs) can perform as miRNA sponges to regulate development in animals. However, the function of pituitary-derived circRNAs in first estrus remains unclear in pigs. In this study, we performed a genome-wide identification and characterization of circRNAs using pituitaries from Landrace × Yorkshire crossbred pigs at three stages: pre-, in-, and post-puberty, to describe such pituitary-derived circRNAs in pigs. A total of 5148 circRNAs were found in the gilts' pituitaries, averaging 18 682 bp in genomic distance, which consisted of approximately 91% exonic, 6% intergenic, and 3% intronic circRNAs. Furthermore, 158 novel circRNAs were identified for the first time and classified as putative pituitary-specific circRNAs. Their expression levels during the onset of puberty, significantly exceeded those of the other circRNAs, and the parental genes of these putative pituitary-specific circRNAs were enriched in "ssc04917: prolactin signaling pathway," "ssc04080: neuroactive ligand-receptor interaction," and "ssc04728: dopaminergic synapse" pathways, all of which were consistent with pituitary functioning. Additionally, 17 differentially regulated circRNAs were found and investigated for their potential interaction with miRNAs, along with genes, by constructing a circRNA-targeted miRNA-gene network. Taken together, these results provide new insight into the circRNA-mediated timing of puberty in gilts at the pituitary level.

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Section: Introductionmentioning

confidence: 99%

Pituitary-Derived Circular RNAs Expression and Regulatory Network Prediction During the Onset of Puberty in Landrace × Yorkshire Crossbred Pigs

et al. 2020

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“…Feeding and slaughtering standards of the pigs were performed as described in previous literature (Chen et al, 2018). As in previous research, piglet weaning was executed at the age of ± days.…”

Section: Animalsmentioning

confidence: 99%

“…Pigs were not allowed to access food one night before slaughtering and had a resting period of 2 h after transportation. To exsanguinate and relief the pain, a sudden electric shock of 90 V and 50 Hz for 10 s was administered (Chen et al, 2018).…”

Section: Animalsmentioning

confidence: 99%

Peer Review #3 of "Comprehensive transcriptional profiling of aging porcine liver (v0.1)"

2019

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Background. Aging is a major risk factor for the development of many diseases, and the liver, as the most important metabolic organ, is significantly affected by aging. It has been shown that the liver weight tends to increase in rodents and decrease in humans with age. Pigs have a genomic structure, physiological as well as biochemical features that are similar to those of humans, and have therefore been used as a valuable model for studying human diseases. The molecular mechanisms of the liver aging of large mammals on a comprehensive transcriptional level remain poorly understood. The pig is an ideal model animal to clearly and fully understand the molecular mechanism underlying human liver aging. Methods. In this study, four healthy female Yana pigs (an indigenous Chinese breed) were investigated: two young sows (180-days-old) and two old sows (8-years-old). High throughput RNA sequencing was performed to evaluate the expression profiles of messenger RNA, long non-coding RNAs, micro RNAs, and circular RNAs during the porcine liver aging process. Gene Ontology (GO) analysis was performed to investigate the biological functions of age-related genes. Results. A number of age-related genes were identified in the porcine liver. GO annotation showed that up-regulated genes were mainly related to immune response, while the down-regulated genes were mainly related to metabolism. Moreover, several lncRNAs and their target genes were also found to be differentially expressed during liver aging. In addition, the multi-group cooperative control relationships and constructed circRNA-miRNA co-expression networks were assessed during liver aging. Conclusions. Numerous age-related genes were identified and circRNA-miRNA co-expression networks that are active during porcine liver aging were constructed. These findings contribute to the understanding of the transcriptional foundations of liver aging and also provide further references that clarify human liver aging at the molecular level.

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“…Cardiac senescence accompanies ageing and may decrease heart function. Chen et al (2018) reported that aging significantly affects the cardiac muscle. By using high throughput RNA sequencing, they found up-regulated circRNAs and 1 down-regulated gene in cardiac muscle during aging.…”

Section: Cardiac Senescencementioning

confidence: 99%

Peer Review #2 of "Circular RNAs as potential biomarkers and therapeutics for cardiovascular disease (v0.1)"

2019

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Circular RNAs (circRNAs) are genetic regulators that were earlier considered as "junk." In contrast to linear RNAs, they have covalently linked ends with no poly-adenylated tails. CircRNAs can act as RNA-binding proteins, sequestering agents, transcriptional regulators, as well as microRNA sponges. In addition, it is reported that some selected circRNAs are transformed into functional proteins. These RNA molecules always circularize through covalent bonds, and their presence has been demonstrated across species. They are usually abundant and stable as well as evolutionarily conserved in tissues(liver, lung, stomach), saliva, exosomes, and blood. Therefore, they have been proposed as the "next big thing" in molecular biomarkers for several diseases, particularly in cancer. Recently, circRNAs have been investigated in cardiovascular diseases (CVD) and reported to play important roles in heart failure, coronary artery disease, and myocardial infarction. Here, we review the recent literature and discuss the impact and the diagnostic and prognostic values of circRNAs in CVD.

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Comprehensive transcriptional landscape of porcine cardiac and skeletal muscles reveals differences of aging

Cited by 40 publications

References 77 publications

Pituitary-Derived Circular RNAs Expression and Regulatory Network Prediction During the Onset of Puberty in Landrace × Yorkshire Crossbred Pigs

Pituitary-Derived Circular RNAs Expression and Regulatory Network Prediction During the Onset of Puberty in Landrace × Yorkshire Crossbred Pigs

Peer Review #3 of "Comprehensive transcriptional profiling of aging porcine liver (v0.1)"

Peer Review #2 of "Circular RNAs as potential biomarkers and therapeutics for cardiovascular disease (v0.1)"

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