Comprehensive two-dimensional HepG2/cell membrane chromatography/monolithic column/time-of-flight mass spectrometry system for screening anti-tumor components from herbal medicines

Chen, Xiaofei; Cao, Yan; Lv, Diya; Zhu, Zhenyu; Zhang, Junping; Chai, Yifeng

doi:10.1016/j.chroma.2012.04.034

Cited by 89 publications

(69 citation statements)

References 42 publications

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“…In recent years, researchers have also found other factors that affect the proliferation of HepG2 cells, such as β(2)-AR agonists (R,R')-fenoterol (Fen) and (R,R')-4-methoxy-1-naphthylfenoterol (MNF), BBR and THP which inhibit growth (25,27). There are many reports concerning the control of HepG2 cell proliferation, and the mechanism is complex.…”

Section: Discussionmentioning

confidence: 97%

Resveratrol suppresses the STAT3 signaling pathway and inhibits proliferation of high glucose-exposed HepG2 cells partly through SIRT1

Zhu

et al. 2013

Oncology Reports

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Hepatocellular carcinoma is the most common type of liver cancer. The risk of hepatocellular carcinoma for type 2 diabetic patients is greater than that for non-diabetic individuals although the mechanism is unclear. The cancer suppressor resveratrol inhibits cancer cell proliferation partly through the STAT3 signaling pathway. However, the effects of resveratrol on STAT3 in high glucose-exposed HepG2 cells and the role of SIRT1 are not clear to date. The aim of the present study was to investigate the effects of resveratrol on STAT3 and SIRT1 regarding the proliferation of high glucose-exposed HepG2 cells. HepG2 cells were cultured in DMEM containing glucose (2.8, 5.5 and 25 mM) and resveratrol (0, 10 and 100 µM). HepG2 cell proliferation and viability were analyzed by MTT assays. The levels of p-STAT3 and SIRT1 were analyzed by western blotting, and RT-PCR methods were used to detect the transcription levels of cyclin B1, cyclin D1, VEGF and MMP-9. SIRT1-specific short-interfering RNA was used to investigate the role of SIRT1 in p-STAT3 signaling. A high glucose concentration (25 mM) induced HepG2 cell proliferation. This effect was suppressed by resveratrol (100 µM), and the effect on the p-STAT3 signaling pathway was found to be SIRT1-dependent. Our findings may provide new insights into the mechanism by which resveratrol suppresses HepG2 cell proliferation under conditions of high glucose. Furthermore, this information may provide the basis for a novel therapeutic strategy for hepatocellular carcinoma patients suffering from either diabetes or hyperglycemia.

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Section: Discussionmentioning

confidence: 97%

Resveratrol suppresses the STAT3 signaling pathway and inhibits proliferation of high glucose-exposed HepG2 cells partly through SIRT1

Zhu

et al. 2013

Oncology Reports

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“…To control the flow rate well and to maintain a constant pressure in the secondary capillary column to suppress bubble formation, a splitting cross connected to a capillary restrictor was utilized. The Reprinted and modified from [78], with permission from Elsevier. system that was constructed, possessing three separation mechanisms, namely, SCX, RP and electrophoresis, could provide high orthogonality to separate complex samples.…”

Section: Other Two-dimensional Chromatography Systemsmentioning

confidence: 98%

“…As a result, more than 40 compounds were well separated on the ILC column with interactive activity. In another approach, Chen et al [78] proposed a novel on-line LC × LC system to screen anti-tumor compounds from several CHMs. Cell membrane chromatography (CMC) was employed in the 1 D using the HepG2 cell membrane as the stationary phase.…”

Section: Comprehensive Lc (Lc × Lc) Systemmentioning

confidence: 99%

Application of two-dimensional chromatography in the analysis of Chinese herbal medicines

Cao

Wei

Chen

et al. 2014

Journal of Chromatography A

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“…13−15 This method had been extensively reviewed. 16,17 It is a practical method for screening active components from complex HMs 18−21 and also for investigating drug-receptor interactions. 22−24 In order to efficiently characterize and identify the active ingredients that have an affinity to cell membrane stationary phase (CMSP), several two-dimensional liquid chromatography (2D-LC) methods have been established for rapid investigation of complex HMs.…”

mentioning

confidence: 99%

“…33,34 In our previous research, a comprehensive two-dimensional CMC system was established for rapid screening of antitumor components from 28 HMs. 21 …”

mentioning

confidence: 99%

Comparative Normal/Failing Rat Myocardium Cell Membrane Chromatographic Analysis System for Screening Specific Components That Counteract Doxorubicin-Induced Heart Failure from Acontium carmichaeli

et al. 2014

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Cell membrane chromatography (CMC) derived from pathological tissues is ideal for screening specific components acting on specific diseases from complex medicines owing to the maximum simulation of in vivo drug-receptor interactions. However, there are no pathological tissue-derived CMC models that have ever been developed, as well as no visualized affinity comparison of potential active components between normal and pathological CMC columns. In this study, a novel comparative normal/failing rat myocardium CMC analysis system based on online column selection and comprehensive two-dimensional (2D) chromatography/monolithic column/time-of-flight mass spectrometry was developed for parallel comparison of the chromatographic behaviors on both normal and pathological CMC columns, as well as rapid screening of the specific therapeutic agents that counteract doxorubicin (DOX)-induced heart failure from Acontium carmichaeli (Fuzi). In total, 16 potential active alkaloid components with similar structures in Fuzi were retained on both normal and failing myocardium CMC models. Most of them had obvious decreases of affinities on failing myocardium CMC compared with normal CMC model except for four components, talatizamine (TALA), 14-acetyl-TALA, hetisine, and 14-benzoylneoline. One compound TALA with the highest affinity was isolated for further in vitro pharmacodynamic validation and target identification to validate the screen results. Voltage-dependent K+ channel was confirmed as a binding target of TALA and 14-acetyl-TALA with high affinities. The online high throughput comparative CMC analysis method is suitable for screening specific active components from herbal medicines by increasing the specificity of screened results and can also be applied to other biological chromatography models.

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Comprehensive two-dimensional HepG2/cell membrane chromatography/monolithic column/time-of-flight mass spectrometry system for screening anti-tumor components from herbal medicines

Cited by 89 publications

References 42 publications

Resveratrol suppresses the STAT3 signaling pathway and inhibits proliferation of high glucose-exposed HepG2 cells partly through SIRT1

Resveratrol suppresses the STAT3 signaling pathway and inhibits proliferation of high glucose-exposed HepG2 cells partly through SIRT1

Application of two-dimensional chromatography in the analysis of Chinese herbal medicines

Comparative Normal/Failing Rat Myocardium Cell Membrane Chromatographic Analysis System for Screening Specific Components That Counteract Doxorubicin-Induced Heart Failure from Acontium carmichaeli

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