Compressibility and specific volume of actin decrease upon G to F transformation

Suzuki, Naoya; Tamura, Youjiro; Mihashi, Koshin

doi:10.1016/0167-4838(95)00213-8

Cited by 23 publications

(16 citation statements)

References 48 publications

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“…The authors explained this small concentration-related difference by interactions of f-actin filaments. According to the present study, the sound velocity of polymerized actin is almost independent of protein concentrations in the range used by Suzuki et al (1996) (Fig. 11).…”

Section: Concentration-dependent Viscoelasticity Of F-actinsupporting

confidence: 68%

“…Sound velocity measurements of polymerized actin were performed by Suzuki et al (1996) with what they called a sing-around pulse method. They observed a decrease of the compressibility during actin polymerization.…”

Section: Concentration-dependent Viscoelasticity Of F-actinmentioning

confidence: 99%

“…Using Eq. 16, we calculated the partial specific adiabatic compressibility, with 0 ϭ 0.632 cm 3 /g as determined by Suzuki et al (1996), 0 ϭ 0.998, u 0 ϭ 149800 cm/s (25°C) and (⌬u/c) lim c3o ϭ Ϫ10 cm/s (derived from the f-actin measurements as shown in Fig. 11 A) and obtained a value of Ϫ6.9 ⅐ 10 Ϫ10 Pa Ϫ1 , which is also negative but two orders of magnitude higher than Ϫ12.7 ⅐ 10 Ϫ12 Pa Ϫ1 , determined by Suzuki et al (1996).…”

Section: Concentration-dependent Viscoelasticity Of F-actinmentioning

confidence: 99%

“…The oscillating rod rheometer (ORR) simultaneously allows calculation of the volume elasticity of a fluid or gel from measurements of the velocity of longitudinal ultrasonic waves and determination of the dynamic viscosity from the oscillations (in a nanometer range) of a glass rod immersed in the viscous fluid. Compressibility of protein solutions reveals packing density and phase transitions (e.g., from liquid to liquid-crystal) and also conformational changes (Suzuki et al, 1996;Tamura et al, 1993;Leung et al, 1986). Therefore, determinations of the compression modulus are methods supplementary to nuclear magnetic resonance (NMR) spectroscopy and fluorescence resonance energy transfer (FRET).…”

Section: Introductionmentioning

confidence: 99%

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Viscoelastic Properties of f-actin, Microtubules, f-actin/α-actinin, and f-actin/Hexokinase Determined in Microliter Volumes with a Novel Nondestructive Method

Wagner

Zinke

Dancker

et al. 1999

Biophysical Journal

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A nondestructive method to determine viscoelastic properties of gels and fluids involves an oscillating glass fiber serving as a sensor for the viscosity of the surrounding fluid. Extremely small displacements (typically 1-100 nm) are caused by the glass rod oscillating at its resonance frequency. These displacements are analyzed using a phase-sensitive acoustic microscope. Alterations of the elastic modulus of a fluid or gel change the propagation speed of a longitudinal acoustic wave. The system allows to study quantities as small as 10 microliters with temporal resolution >1 Hz. For 2-100 microM f-actin gels a final viscosity of 1.3-9.4 mPa s and a final elastic modulus of 2.229-2.254 GPa (corresponding to 1493-1501 m/s sound velocity) have been determined. For 10- to 100-microM microtubule gels (native, without stabilization by taxol), a final viscosity of 1.5-124 mPa s and a final elastic modulus of 2.288-2. 547 GPa (approximately 1513-1596 m/s) have been determined. During polymerization the sound velocity in low-concentration actin solutions increased up to +1.3 m/s (approximately 1.69 kPa) and decreased up to -7 m/s (approximately 49 kPa) at high actin concentrations. On polymerization of tubulin a concentration-dependent decrease of sound velocity was observed, too (+48 to -12 m/s approximately 2.3-0.1 MPa, for 10- to 100-microM tubulin). This decrease was interpreted by a nematic phase transition of the actin filaments and microtubules with increasing concentration. 2 mM ATP (when compared to 0.2 mM ATP) increased polymerization rate, final viscosity and elastic modulus of f-actin (17 microM). The actin-binding glycolytic enzyme hexokinase also accelerated the polymerization rate and final viscosity but elastic modulus (2.26 GPa) was less than for f-actin polymerized in presence of 0.2 mM ATP (2.28 GPa).

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Section: Concentration-dependent Viscoelasticity Of F-actinsupporting

confidence: 68%

Section: Concentration-dependent Viscoelasticity Of F-actinmentioning

confidence: 99%

Section: Concentration-dependent Viscoelasticity Of F-actinmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Viscoelastic Properties of f-actin, Microtubules, f-actin/α-actinin, and f-actin/Hexokinase Determined in Microliter Volumes with a Novel Nondestructive Method

Wagner

Zinke

Dancker

et al. 1999

Biophysical Journal

View full text Add to dashboard Cite

show abstract

“…Ultrasound has been used for many years in the study of proteins (Owen and Simons, 1957;Conway and Verral, 1966;Pavlovskaya et al, 1992;Suzuki et al, 1996). These studies have been used to estimate protein hydration and to infer changes in protein conformation.…”

Section: Introductionmentioning

confidence: 99%