Compression-induced hyperaemia in the rabbit masseter muscle: a model to investigate vascular mechano-sensitivity of skeletal muscle

Turturici, Marco; Roatta, Silvestro

doi:10.1088/0967-3334/34/3/307

Cited by 5 publications

(6 citation statements)

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“…The lack of this local regulatory mechanism in isolated vessel preparations likely explains why, when multiple compressive stimuli are delivered to isolated feed arteries, a potentiation of the dilatation is observed ( Clifford et al, 2006 ) rather than an attenuation, as reported in the present and other studies in humans and intact animals ( Tschakovsky et al, 1996 ; Turturici and Roatta, 2013a ; Messere et al, 2017a , b ).…”

Section: Discussionsupporting

confidence: 65%

“…The hyperemic response to MC was previously shown to exhibit a progressive attenuation in response to repetitive stimulation. This was first observed in the masseter muscle of the anesthetized rabbit ( Turturici and Roatta, 2013a , b ) and was later confirmed in the human forearm ( Messere et al, 2017b ). The effect was initially attributed to the possible inactivation of the putative mechano-sensitive vascular structures ( Turturici and Roatta, 2013b ; Messere et al, 2017b ) according to similar inactivation patterns described for vascular mechano-sensitive structures ( Honore et al, 2006 ; Earley and Brayden, 2015 ).…”

Section: Introductionmentioning

confidence: 70%

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Hyper-Oxygenation Attenuates the Rapid Vasodilatory Response to Muscle Contraction and Compression

et al. 2018

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A single muscle compression (MC) with accompanying hyperemia and hyper-oxygenation results in attenuation of a subsequent MC hyperemia, as long as the subsequent MC takes place when muscle oxygenation is still elevated. Whether this is due to the hyper-oxygenation, or compression-induced de-activation of mechano-sensitive structures is unclear. We hypothesized that increased oxygenation and not de-activation of mechano-sensitive structures was responsible for this attenuation and that both compression and contraction-induced hyperemia attenuate the hyperemic response to a subsequent muscle contraction, and vice-versa. Protocol-1) In eight subjects two MCs separated by a 25 s interval were delivered to the forearm without or with partial occlusion of the axillary artery, aimed at preventing hyperemia and increased oxygenation in response to the first MC. Tissue oxygenation [oxygenated (hemoglobin + myoglobin)/total (hemoglobin + myoglobin)] from forearm muscles and brachial artery blood flow were continuously monitored by means of spatially-resolved near-infrared spectroscopy (NIRS) and Doppler ultrasound, respectively. With unrestrained blood flow, the hyperemic response to the second MC was attenuated, compared to the first (5.7 ± 3.3 vs. 14.8 ± 3.9 ml, P < 0.05). This attenuation was abolished with partial occlusion of the auxillary artery (14.4 ± 3.9 ml). Protocol-2) In 10 healthy subjects, hemodynamic changes were assessed in response to MC and electrically stimulated contraction (ESC, 0.5 s duration, 20 Hz) of calf muscles, as single stimuli or delivered in sequences of two separated by a 25 s interval. When MC or ESC were delivered 25 s following MC or ESC the response to the second stimulus was always attenuated (range: 60–90%). These findings support a role for excess tissue oxygenation in the attenuation of mechanically-stimulated rapid dilation and rule out inactivation of mechano-sensitive structures. Furthermore, both MC and ESC rapid vasodilatation are attenuated by prior transient hyperemia, regardless of whether the hyperemia is due to MC or ESC. Previously, mechanisms responsible for this dilation have not been considered to be oxygen sensitive. This study identifies muscle oxygenation state as relevant blunting factor, and reveals the need to investigate how these feedforward mechanisms might actually be affected by oxygenation.

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Section: Discussionsupporting

confidence: 65%

Section: Introductionmentioning

confidence: 70%

Hyper-Oxygenation Attenuates the Rapid Vasodilatory Response to Muscle Contraction and Compression

et al. 2018

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“…muscle blood flow; hyperemia; muscle compression; tissue oxygenation SINCE THE SEMINAL WORK of Mohrman and Sparks (39) several studies have demonstrated that a rapid and transient hyperemic response can be elicited by a short-lasting muscle compression (10, 30, 38, 56 -60). Although the underlying mechanisms have not been fully identified, this phenomenon has been well documented in different experimental models, such as the isolated muscle (39), awake and anesthetized animals (57,58,60), and humans (10,30,38,56). In addition, a rapid dilatory response to compressive stimuli has also been observed in isolated feed arteries (7).…”

mentioning

confidence: 97%

Increased tissue oxygenation explains the attenuation of hyperemia upon repetitive pneumatic compression of the lower leg

Messere

Ceravolo

Franco

et al. 2017

Journal of Applied Physiology

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The rapid hyperemia evoked by muscle compression is short lived and was recently shown to undergo a rapid decrease even in spite of continuing mechanical stimulation. The present study aims at investigating the mechanisms underlying this attenuation, which include local metabolic mechanisms, desensitization of mechanosensitive pathways, and reduced efficacy of the muscle pump. In 10 healthy subjects, short sequences of mechanical compressions ( = 3-6; 150 mmHg) of the lower leg were delivered at different interstimulus intervals (ranging from 20 to 160 s) through a customized pneumatic device. Hemodynamic monitoring included near-infrared spectroscopy, detecting tissue oxygenation and blood volume in calf muscles, and simultaneous echo-Doppler measurement of arterial (superficial femoral artery) and venous (femoral vein) blood flow. The results indicate that ) a long-lasting (>100 s) increase in local tissue oxygenation follows compression-induced hyperemia, ) compression-induced hyperemia exhibits different patterns of attenuation depending on the interstimulus interval,) the amplitude of the hyperemia is not correlated with the amount of blood volume displaced by the compression, and ) the extent of attenuation negatively correlates with tissue oxygenation ( = -0,78, < 0.05). Increased tissue oxygenation appears to be the key factor for the attenuation of hyperemia upon repetitive compressive stimulation. Tissue oxygenation monitoring is suggested as a useful integration in medical treatments aimed at improving local circulation by repetitive tissue compression. This study shows that ) the hyperemia induced by muscle compression produces a long-lasting increase in tissue oxygenation,) the hyperemia produced by subsequent muscle compressions exhibits different patterns of attenuation at different interstimulus intervals, and ) the extent of attenuation of the compression-induced hyperemia is proportional to the level of oxygenation achieved in the tissue. The results support the concept that tissue oxygenation is a key variable in blood flow regulation.

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“…Vascular reactivity to mechanical compression takes the form of a rapid dilatation and results in a prompt and short lasting hyperemia. This phenomenon has been originally observed in the isolated muscle (Mohrman and Sparks 1974) and has also been documented in isolated feed arteries (Clifford et al 2006), in intact animals (Turturici et al 2012;Turturici and Roatta 2013a) and in humans, in both upper (Kirby et al 2007;Messere et al 2017b) and lower limbs (Credeur et al 2015;Messere et al 2017a). This mechano sensitivity of the muscle vasculature is likely to contribute also to the hyperemia that develops with passive movements (Wray et al 2005) as well as with muscle contraction (Kirby et al 2007;Messere et al 2017a) and is considered to have a role in the hyperemia at the beginning of exercise (Clifford 2007).…”

Section: Introductionmentioning

confidence: 68%

Delivery of customizable compressive patterns to human limbs to investigate vascular reactivity

Messere¹,

Pertusio²,

Macrì³

et al. 2018

Biomed. Phys. Eng. Express

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ObjectiveCommercial devices for pneumatic compressive treatment of the limbs generally provide predefined stereotyped compressive profiles. The possibility to deliver compressive stimuli with a customizable pressure profile would be useful to differently probe the vascular reactivity to muscle compression (MC) and improve the understanding of MC-induced hyperemia. Aim of this study was the realization of a novel pneumatic system capable of generating adjustable and stable compressive conditions, preceding and following a "standard" MC stimulus Approach A custom-made pneumatic system specifically built to this purpose is tested and characterized in 10 subjects.Three different compressive patterns were delivered to the leg: 1) a constant level: 50 mmHg for 50 s; 2) MC: 200 mmHg for 1 s; 3) a complex profile: 20 mmHg for 50 s, 200 mmHg for 1 s, 50 mmHg for 50 s. Main resultsThe implemented system allowed to deliver graded compressions to the limb characterized by fast transitions (0 to 200 mmHg in 0.5 ± 0.07 s) and stable plateau levels (50.4 ± 0.5 mmHg). SignificanceA new, low-cost, pneumatic prototype has been presented and tested in the present study allowing to deliver compressive stimuli with pre-and post-compressions of adjustable level. This device has been conceived for research purposes and may find application in therapeutic compressive treatments.

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Compression-induced hyperaemia in the rabbit masseter muscle: a model to investigate vascular mechano-sensitivity of skeletal muscle

Abstract: In conclusion, for its high sensitivity and reliability this technique is adequate to characterize mechano-vascular reactivity and may prove useful in the investigation of the underlying mechanisms, with implications in the control of vascular tone and blood pressure in health and disease.

Cited by 5 publications

References 19 publications

Hyper-Oxygenation Attenuates the Rapid Vasodilatory Response to Muscle Contraction and Compression

Hyper-Oxygenation Attenuates the Rapid Vasodilatory Response to Muscle Contraction and Compression

Increased tissue oxygenation explains the attenuation of hyperemia upon repetitive pneumatic compression of the lower leg

Delivery of customizable compressive patterns to human limbs to investigate vascular reactivity

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