Compressive force stimulates the expression of osteogenesis-related transcription factors in ROS 17/2.8 cells

Boning up on Wolff's Law: Mechanical regulation of the cells that make and maintain bone

“…After 1-2 weeks of mechanical loading, mineralization and osteocalcin and OPN protein expression were also significantly increased compare to static controls (Duty et al, 2007;Kim et al, 2007;Rath et al, 2008;Yanagisawa et al, 2008). Notably, the osteogenic response is dependent on the magnitude of mechanical stress in a biphasic manner, indicating that there exists an optimal range of stress for inducing osteogenic differentiation (Sim et al, 2007;Yanagisawa et al, 2007Yanagisawa et al, , 2008Rath et al, 2008). However, the optimal range of stress has yet to be defined, as reported optimal stresses vary from 49 Pa to 11.8 kPa.…”

“…Mechanical stress significantly induced ALP activity and mRNA expression of Runx2, BMP2, osterix, collagen I, osteocalcin, osteonectin, and OPN in osteoprogenitors (Kim et al, 2007;Sim et al, 2007;Yanagisawa et al, 2007;Rath et al, 2008;Wagner et al, 2008;Liu et al, 2009a, b). After 1-2 weeks of mechanical loading, mineralization and osteocalcin and OPN protein expression were also significantly increased compare to static controls (Duty et al, 2007;Kim et al, 2007;Rath et al, 2008;Yanagisawa et al, 2008). Notably, the osteogenic response is dependent on the magnitude of mechanical stress in a biphasic manner, indicating that there exists an optimal range of stress for inducing osteogenic differentiation (Sim et al, 2007;Yanagisawa et al, 2007Yanagisawa et al, , 2008Rath et al, 2008).…”

High-magnitude mechanical strain inhibits the differentiation of bone-forming rat calvarial progenitor cells

“…Although the effect of mechanical strain on the expression of matrix proteins and transcription factors has been investigated (12)(13)(14)18), no studies have evaluated bone nodule formation phenotypically in detail by measuring bone nodule number and size in response to mechanical strain.…”

Compressive Force Induces the Expression of Bone Remodeling-Related Proteins via Interleukin-11 Production in MC3T3-E1 Cells

“…The cells were then compressed continuously using a uniform compression method similar to those described previously 17,[23][24][25][26][27][28][29] . Briefly, thin, round glass plates were placed over the confluent cell layer, and the CF was adjusted by placing a lead weight on the glass plates.…”

“…Three stainless-steel wire bridges (angle 120°) were placed on the glass plates so that the weight did not touch the culture medium. The cells were subjected to 1.0 or 2.0 g/cm 2 (98 or 196 Pa, respectively) of CF for 1, 3, 6, 9, 12, or 24 h [22][23][24][26][27][28][29] . Control cells were covered with thin glass plates without lead weights, which produced a CF of 0.035 g/cm 2 .…”