Computation of coupled diffusion of oxygen, glucose and lactic acid in an intervertebral disc

Soukane, D. Mokhbi; Shirazi-Adl, A.; Urban, Jill

doi:10.1016/j.jbiomech.2007.01.003

Cited by 103 publications

(70 citation statements)

References 53 publications

(78 reference statements)

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“…where R(C) is the production rate (positive) of metabolic byproducts or consumption rate (negative) of nutrient species, respectively, given in concentration units/h, C is the solute concentration (kPa for oxygen, nmol/mm 3 for glucose and lactate) and D is the diffusivity (mm 2 /h) [32,33] (see Table 1 for input data). The model is subject to boundary conditions (Fig.…”

Section: Finite Element Modelmentioning

confidence: 99%

“…As described earlier [32,33], the convergence of the present steady-state case was hardly reached due to severe nonlinearities; therefore a pseudo-transient approach was employed by adding a capacity matrix (CM) to the conventional matrix (K) while using the implicit method. The final system in the matrix form is given as:…”

Section: Finite Element Modelmentioning

confidence: 99%

“…Earlier model studies [18,32,33,42] have been limited to axisymmetric geometries of the IVD that, amongst others, neglect disc height variations present in the sagittal plane especially at lower lumbar discs. These geometries also place restrictions on realistic simulation of conditions of interest such as local endplate fractures/calcifications and changes in the posture.…”

Section: Introductionmentioning

confidence: 99%

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Investigation of solute concentrations in a 3D model of intervertebral disc

2008

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As the disc is the largest avascular structure in the body, disc cells depend for their normal function on an adequate supply of nutrients (oxygen and glucose) and the removal of metabolic by-products (lactic acid) via blood vessels at the cartilaginous endplates and annulus periphery. Concentration gradients develop depending on the balance between the rates of transport and rates of cellular activity. Since consumption and production rates are coupled via extracellular pH, the gradients are interdependent. This is a novel model study which takes into account the realistic 3D geometry of a L5-S1 lumbar disc in solving the nonlinear coupled diffusion equations. Effects of perturbations (calcification, sclerosis) in endplates, increases in cell metabolic rates following growth factor injection and changes in lumbar posture (kyphotic or lordotic) on extreme values of nutrient and metabolite concentrations and their spatial locations are investigated. Solute concentrations, particularly those of glucose, substantially diminish as a consequence of disturbances in supply at the endplates, increases in cell metabolic rate and more lordotic postures. Results, when compared to those from simplified axisymmetric models, demonstrate the importance of consideration of realistic 3D disc geometry.

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Section: Finite Element Modelmentioning

confidence: 99%

Section: Finite Element Modelmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Investigation of solute concentrations in a 3D model of intervertebral disc

2008

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“…Tissue culture and mathematical based studies have clearly established the importance of adequate oxygen (Kumar et al, 2004;Wang et al, 2008) and glucose delivery (Boubriak et al, 2006;Converti et al, 1996;Mokhbi Soukane et al, 2007) for adequate cell activity and tissue formation and noted that any unmet demand of nutrients can lead to low cell proliferation, and low collagen production particularly at the center of scaffolds with low porosities (Ishaug-Riley et al, 1998;Patnaik et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

“…(Boubriak et al, 2006;Converti et al, 1996;Day et al, 2013;Gao and Xu, 2011;Koutny, 2011a;Koutny, 2011b;Martineau, 2012;Mokhbi Soukane et al, 2007).…”

Section: Chapter 5: Mass Transport Modelmentioning

confidence: 99%

Movement Effects on the Flow Physics and Nutrient Delivery in Engineered Valvular Tissues

Salinas¹

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Enzymatically Bioactive Nucleus Pulposus Matrix Hydrogel Microspheres for Exogenous Stem Cells Therapy and Endogenous Repair Strategy to Achieve Disc Regeneration

Peng,

Chen,

Zhang

et al. 2023

Advanced Science

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Exogenous stem cell therapy and endogenous repair has shown great potential in intervertebral disc regeneration. However, limited nutrients and accumulation of lactate largely impair the survival and regenerative capacity of implanted stem cells and endogenous nucleus pulposus cells (NPCs). Herein, an injectable hydrogel microsphere (LMGDNPs) have been developed by immersing lactate oxidase (LOX)‐manganese dioxide (MnO2) nanozyme (LM) into glucose‐enriched decellularized nucleus pulposus hydrogel microspheres (GDNPs) through a microfluidic system. LMGDNPs showed a delayed release profile of LOX and satisfactory enzymatic capacity in consuming lactate. Mesenchymal stem cells (MSCs) plated on LMGDNPs exhibited better cell viability than cells on GelMA and decellularized nucleus pulposus microspheres (DNP) and showed a obviously increased NPCs phenotype. LMGDNPs prevented MSCs and NPCs death and promoted extracellular matrix synthesis by exhausting lactate. It is determined that LMGDNPs promoted NPCs autophagy by activating transforming growth factor β2 overlapping transcript 1 (TGFB2‐OT1), relying on the nanozyme. MSCs‐loaded LMGDNPs largely preserved disc hydration and alleviated matrix degradation in vivo. Summarily, LMGDNPs promoted cell survival and matrix regeneration by providing a nutrient supply, exhausting lactate, and activating autophagy via TGFB2‐OT1 and its downstream pathway and may serve as an ideal delivery system for exogenous stem cell therapy and endogenous repair.

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Computation of coupled diffusion of oxygen, glucose and lactic acid in an intervertebral disc

Cited by 103 publications

References 53 publications

Investigation of solute concentrations in a 3D model of intervertebral disc

Investigation of solute concentrations in a 3D model of intervertebral disc

Movement Effects on the Flow Physics and Nutrient Delivery in Engineered Valvular Tissues

Enzymatically Bioactive Nucleus Pulposus Matrix Hydrogel Microspheres for Exogenous Stem Cells Therapy and Endogenous Repair Strategy to Achieve Disc Regeneration

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