Computational Algorithm to Assess Genetic Relationship and Functional Analysis of Non-Synonymous Substitution of HSP 70 Gene of Cattle, Sheep and Goat

Abstract

This study was carry out using heat shock protein 70 (HSP 70) sequences comprising goat (5), sheep (5) and cattle (5) which was obtained from the GenBank (NCBI). Protein Variant Effect Analyzer (PROVEAN) tool was used to check the nonsynonymous single nucleotide polymorphism of the HSP 70 gene of amino acid substitution for cattle, goat and sheep. The study observed that some amino acid substitution returned neutral/beneficial while some deleterious/harmful. Molecular evolutionary genetics analysis (MEGA) 7.0 was used to perform both Tajima's test and phylogenetic relationship for cattle, goat and sheep. The Tajima's test (D) for all the species revealed positive values, an indication of purifying selection. The phylogenetic relationship followed bovidae family speciation. The study concluded that information emanating from this research is significant for further dry and wet laboratory experiment.