Computational analysis of a species D human adenovirus provides evidence of a novel virus

Espínola, Emilio E.; Barrios, Julio César Quiroz; Russomando, Graciela; Mirazo, Santiago

doi:10.1099/jgv.0.000947

Cited by 18 publications

(8 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Human adenovirus is well-recognized as an important pathogen of respiratory tract infection in childhood [1–3]. More than 70 HAdV serotypes are further subdivided into seven species (A-G) [4, 5]; however, species B (HAdV-3,7,11 and 14), C (HAdV-1,2,5 and 6), and E (HAdV-4) are the ones most associated with respiratory infections [6]. Epidemiological studies have reported an approximately 5–10% HAdV-positive rate among acute respiratory tract infections in children [7, 8].…”

Section: Introductionmentioning

confidence: 99%

Human adenovirus type 7 infection causes a more severe disease than type 3

Tang

et al. 2019

BMC Infect Dis

View full text Add to dashboard Cite

BackgroundHuman adenovirus type 3 (HAdV-3) and 7 (HAdV-7) cause significant morbidity and develop severe complications and long-term pulmonary sequelae in children. However, epidemiologic reports have suggested that nearly all highly severe or fatal adenoviral diseases in children are associated with HAdV-7 rather than HAdV-3. Here, we conduct in-depth investigations to confirm and extend these findings through a comprehensive series of assays in vitro and in vivo as well as clinical correlates.MethodsA total of 8248 nasopharyngeal aspirate (NPA) samples were collected from hospitalized children with acute respiratory infections in Children’s Hospital of Chongqing Medical University from June 2009 to May 2015. Among 289 samples that tested positive for HAdVs, clinical data of 258 cases of HAdV-3 (127) and HAdV-7 (131) infections were analyzed. All HAdV-positive samples were classified by sequencing the hexon and fiber genes, and compared with clinical data and virological assays. We also performed in vitro assays of virus quantification, viral growth kinetics, competitive fitness, cytotoxicity and C3a assay of the two strains. Mouse adenovirus model was used to evaluate acute inflammatory responses.ResultsClinical characteristics revealed that HAdV-7 infection caused more severe pneumonia, toxic encephalopathy, respiratory failure, longer mean hospitalization, significantly lower white blood cell (WBC) and platelet counts, compared to those of HAdV-3. In cell culture, HAdV-7 replicated at a higher level than HAdV-3, and viral fitness showed significant differences as well. HAdV-7 also exhibited higher C3a production and cytotoxic effects, and HAdV-7-infected mice showed aggravated pathology and higher pulmonary virus loads, compared to HAdV-3-infected mice. Macrophages in BALF remained markedly high during infection, with concomitant increase in pro-inflammatory cytokines (TNF-α, IL-1β, IFN-γ, and IL-6), compared HAdV-3 infection.ConclusionsThese results document that HAdV-7 replicates more robustly than HAdV-3, and promotes an exacerbated cytokine response, causing a more severe airway inflammation. The findings merit further mechanistic studies that offer the pediatricians an informed decision to proceed with early diagnosis and treatment of HAdV-7 infection.

show abstract

Section: Introductionmentioning

confidence: 99%

Human adenovirus type 7 infection causes a more severe disease than type 3

Tang

et al. 2019

BMC Infect Dis

View full text Add to dashboard Cite

show abstract

“…Very young children, elderly persons with underlying respiratory or cardiac disease, or immunocompromised persons are at high risk for severe HAdV infection, leading to multi‐system organ failure and death . More than 70 HAdV types divided into seven species (A to G) have been described; however, most common respiratory infections are associated with species B, C, and E . The most commonly detected HAdV types in the in the United States are C1, C2, B3, and C5 .…”

Section: Introductionmentioning

confidence: 99%

Adenovirus types associated with severe respiratory diseases: A retrospective 4‐year study in Kuwait

Chehadeh

Al-Adwani

John

et al. 2018

Journal of Medical Virology

View full text Add to dashboard Cite

Human adenovirus (HAdV) infection can result in a severe respiratory disease. The aim of this study was to identify HAdV types detected in patients hospitalized for severe respiratory illness. The study population consisted of 743 patients with severe respiratory disease admitted to four major hospitals in Kuwait between January 2013 and December 2016. Respiratory specimens were retrospectively screened for 20 respiratory viruses by real-time PCR. The HAdV hexon gene was amplified and directly sequenced, and HAdV types were identified by performing Bayesian phylogenetic analysis. HAdV DNA was detected in 27 (3.6%) patients, with peaks in November and March. Most patients were infants and young children suffering from pneumonia or acute bronchiolitis. The detected HAdV types were C1, C2, C5, B3, and B7. Clusters of HAdV C1, C2, and C5 were observed with high posterior probability. All patients infected with HAdV C5 and 50% of patients infected with HAdV C2 or B7 were admitted to the intensive care unit (ICU). Co-infection with other viruses was detected in 44.4% of patients. The most common co-infecting virus was rhinovirus (HRV). HAdV/HRV co-infection was detected in two children who presumably developed disseminated HAdV infection and died. This is the first report describing the circulation of HAdV types associated with severe outcomes in Kuwait. These findings highlight the need for a national surveillance system to monitor changes in predominant HAdV types and increased numbers of severe respiratory infections.

show abstract

“…Adenovirus is a DNA virus responsible for varieties of human diseases that affect the gastrointestinal tract, respiratory tract, and eye [1]. There are seven species for adenovirus categorized from A to G with two subgroups [2,3]. The classification correlates with clinical presentation, antigenic like symptoms to severe infections with pneumonia and high mortality up to 50% in immunocompromised patients [9].…”

Section: Introductionmentioning

confidence: 99%

Clinico-Pathological Study of Adenovirus Associated with Respiratory Infections in Children

Zaki¹,

Eid²,

Faried³

2020

TOMICROJ

View full text Add to dashboard Cite

Background: Adenovirus is associated with respiratory tract infections in children worldwide. However, there is insufficient data about adenovirus infections in Egyptian children and the genotypes present in this infection. Objective: The aim of the present study was to investigate the prevalence of adenovirus and its genotypes in respiratory tract infection in children by real-time Polymerase Chain Reaction (PCR). Methods: The study was a cross-sectional study that included 100 children complaining of respiratory tract infections signs and symptoms. Laboratory investigation for adenovirus included real-time polymerase chain reaction and genotypes detection by Multiplex Polymerase Chain Reaction (PCR). Results: Adenovirus was detected by PCR for fiber gene in 11% with genotype 3 in 6 samples (54.5%) and genotype 7 in 5 samples (45.5%) positive for adenovirus by Multiplex PCR. The main presenting symptoms and signs in children with adenovirus detected by PCR were cough, fever, wheezing, and croups (90.9%, 81.1%, 63.6%, and 63.6%, respectively). The diagnosis in children with adenovirus was pneumonia in 72.7% and bronchitis in 27.7%. There were statistically insignificant differences in demographic, clinical, and hematological parameters between children with adenovirus and children negative to adenovirus by PCR. Conclusion: The clinical characteristics of respiratory infections with adenovirus vary upon the age of the patients and the immune status. Therefore, there is a requirement for an extensive study of adenovirus in respiratory infections in children with different ages and immune status.

show abstract

Computational analysis of a species D human adenovirus provides evidence of a novel virus

Cited by 18 publications

References 22 publications

Human adenovirus type 7 infection causes a more severe disease than type 3

Human adenovirus type 7 infection causes a more severe disease than type 3

Adenovirus types associated with severe respiratory diseases: A retrospective 4‐year study in Kuwait

Clinico-Pathological Study of Adenovirus Associated with Respiratory Infections in Children

Contact Info

Product

Resources

About