Computational characterization of epitopic region within the outer membrane protein candidate in<i>Flavobacterium columnare</i>for vaccine development

Bhattacharya, Manojit; Malick, Ramesh Chandra; Mondal, Niladri; Patra, Prasanta; Pal, Bibhuti Bhusan; Patra, Bidhan Chandra; Das, Basanta Kumar

doi:10.1080/07391102.2019.1580222

Cited by 25 publications

(16 citation statements)

References 50 publications

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“…This epitope-based vaccine development approach has been explored and proved to be promising in a number of diseases caused by viruses like Herpes simplex virus (Hasan et al, 2019;Kumar et al, 2019), Yellow Fever Virus (Tosta et al, 2019), SARS Coronavirus (Srivastava et al, 2019), Chikungunya (Bappy et al, 2020), Dengue (Sabetian, Nezafat, Dorosti, Zarei, & Ghasemi, 2019), Alkhurma hemorrhagic fever virus (Ul-Rahman & Shabbir, 2019), etc. as well as by other pathogens including Leishmania donovani (Khatoon et al, 2019), Elizabethkingia anophelis (Nain et al, 2019), Flavobacterium columnare (Bhattacharya et al, 2020), Bacillus anthracis (Gupta, Khatoon, Mishra, Verma, & Prajapati, 2019), Streptococcus pneumoniae (Dorosti et al, 2019), Helicobacter pylori (Pasala et al, 2019), etc. Thus, this multi-epitope vaccine construct designed with a multi-dimensional approach helps in the activation of TLR3 along with the innate and specific adaptive immune response that will also be adsorbed from the intestinal lining and will generate a prominent immune response against the deadly Nipah virus.…”

Section: Introductionmentioning

confidence: 99%

Designing of a multi-epitope vaccine candidate against Nipah virus by in silico approach: a putative prophylactic solution for the deadly virus

Majee

Jain

Kumar

2020

Journal of Biomolecular Structure and Dynamics

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Nipah virus (NPV) is one of the most notorious viruses with a very high fatality rate. Because of the recurrent advent of this virus and its severe neurological implications, often leading to high mortality, the WHO R&D Blueprint, 2018 has listed the Nipah virus as one of the emerging infectious diseases requiring urgent research and development effort. Yet there is a major layback in the development of effective vaccines or drugs against NPV. In this study, we have designed a stable multivalent vaccine combining several T-cell and B-cell epitopes of the essential Nipah viral proteins with the help of different ligands and adjuvants which can effectively induce both humoral and cellular immune responses in human. Different advanced immune-informatic tools confirm the stability, high immunogenicity and least allergenicity of the vaccine candidate. The standard molecular dynamic cascade analysis validates the stable interaction of the vaccine construct with the human Toll-like receptor 3 (TLR3) complex. Later, codon optimization and in silico cloning in a known pET28a vector system shows the possibility for the expression of this vaccine in a simple organism like E.coli. It is believed that with further in vitro and in vivo validation, this vaccine construct can pose to be a better prophylactic solution to the Nipah viral disease.

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Section: Introductionmentioning

confidence: 99%

Designing of a multi-epitope vaccine candidate against Nipah virus by in silico approach: a putative prophylactic solution for the deadly virus

Majee

Jain

Kumar

2020

Journal of Biomolecular Structure and Dynamics

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“…The protein structure is submitted to the Chrion ( http://redshift.med.unc.edu/chiron/index.php ) server for model optimization, and the optimized model was submitted to the structural analysis and verification server SAVES ( http://servicesn.mbi.ucla.edu/SAVES/ ), which validate the model using the optional PROCHECK program ( Bhattacharya et al, 2019 ).…”

Section: Methodsmentioning

confidence: 99%

The Active Ingredients Identification and Antidiarrheal Mechanism Analysis of Plantago asiatica L. Superfine Powder

Dong

et al. 2021

Front. Pharmacol.

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Plantago asiatica L. is a natural medicinal plant that has been widely used for its various pharmacological effects such as antidiarrheal, anti-inflammatory, and wound healing. This study aims to explore the antidiarrheal active ingredients of Plantago asiatica L. that can be used as quality markers to evaluate P. asiatica L. superfine powder (PSP). Molecular docking experiment was performed to identify the effective components of P. asiatica L., which were further evaluated by an established mouse diarrhea model. Na+/K+-ATPase and creatine kinase (CK) activities and the Na+/K+ concentrations were determined. The gene expression of ckb and Atp1b3 was detected. PSP was prepared and evaluated in terms of the tap density and the angle of repose. The structures of PSPs of different sizes were measured by infrared spectra. The active ingredient contents of PSPs were determined by HPLC. The results indicated that the main antidiarrheal components of P. asiatica L. were luteolin and scutellarein that could increase the concentration of Na+ and K+ by upregulating the activity and gene level of CK and Na+/K+-ATPase. In addition, luteolin and scutellarein could also decrease the volume and weight of small intestinal contents to exert antidiarrheal activity. Moreover, as the PSP size decreased from 6.66 to 3.55 μm, the powder tended to be amorphous and homogenized and of good fluidity, the content of active compounds gradually increased, and the main structure of the molecule remained steady. The optimum particle size of PSP with the highest content of active components was 3.55 μm, and the lowest effective dose for antidiarrhea was 2,000 mg/kg. Therefore, the antidiarrheal active ingredients of PSP were identified as luteolin and scutellarein that exert antidiarrheal activity by binding with Na+/K+-ATPase. PSP was successfully prepared and could be used as a new dosage form for the diarrhea treatment.

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“…The presence of a T-cell epitope within a protein chain can initiate cell-mediated cytotoxicity. Instead of separating B and T-cell epitope, the common epitopes are much more effective in an immune system (Bhattacharya et al 2019 ). To get the common B and T-cell epitopes, identified B-cell epitopes are processed through the Proped-I and Proped servers for MHC-I and MHC-II molecule, respectively (Bhasin and Raghava 2007 ; Dikhit et al 2017 , 2018 ; Singh and Raghava 2003 ).…”

Section: Methodsmentioning

confidence: 99%

Evaluation and Designing of Epitopic-Peptide Vaccine Against Bunyamwera orthobunyavirus Using M-Polyprotein Target Sequences

Ghosh

Bhattacharya

Patra

et al. 2021

Int J Pept Res Ther

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Computational characterization of epitopic region within the outer membrane protein candidate inFlavobacterium columnarefor vaccine development

Cited by 25 publications

References 50 publications

Designing of a multi-epitope vaccine candidate against Nipah virus by in silico approach: a putative prophylactic solution for the deadly virus

Designing of a multi-epitope vaccine candidate against Nipah virus by in silico approach: a putative prophylactic solution for the deadly virus

The Active Ingredients Identification and Antidiarrheal Mechanism Analysis of Plantago asiatica L. Superfine Powder

Evaluation and Designing of Epitopic-Peptide Vaccine Against Bunyamwera orthobunyavirus Using M-Polyprotein Target Sequences

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