Computational framework for the techno-economic analysis of monoclonal antibody capture chromatography platforms

Romero, Juan J; Jenkins, Eleanor W.; Osuofa, Joshua; Husson, Scott M.

doi:10.1016/j.chroma.2022.463755

Cited by 2 publications

(2 citation statements)

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“…Romero et al (2023) developed a thorough computational framework to evaluate the performance of antibody capture processes and considered the use of PrismA resin chromatography column, a Purexa™ PrA cast membrane, and a Fibro™ PrismA nonwoven fabric membrane. The results showed that in single‐cycle operations the cost of goods of the resin approach was 28%–30% lower than those of either membrane.…”

Section: Discussionmentioning

confidence: 99%

“…Most critically, it has been shown that 100% recovery can be achieved at low retention times, though high ligand densities have been found to negatively impact capsid infectivity. Because the yields of many viral vector production systems are very low, the limited capacity of nonwovens compared to resins is not a significant negative factor, and the 29-fold increase in productivity over resin systems would result in significant cost savings.8 | DISCUSSION AND CONCLUSIONSRomero et al (2023) developed a thorough computational framework to evaluate the performance of antibody capture processes and considered the use of PrismA resin chromatography column, a…”

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confidence: 99%

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Advances in high‐throughput, high‐capacity nonwoven membranes for chromatography in downstream processing: A review

Lavoie

Fan

Pourdeyhimi

et al. 2023

Biotech & Bioengineering

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Nonwoven membranes are highly engineered fibrous materials that can be manufactured on a large scale from a wide range of different polymers, and their surfaces can be modified using a large variety of different chemistries and ligands. The fiber diameters, surface areas, pore sizes, total porosities, and thicknesses of the nonwoven mats can be carefully controlled, providing many opportunities for creative approaches for the development of novel membranes with unique properties to meet the needs of the future of downstream processing. Fibrous membranes are already finding use in ultrafiltration, microfiltration, depth filtration, and, more recently, in membrane chromatography for product capture and impurity removal. This article summarizes the various methods of manufacturing nonwoven fabrics, and the many methods available for the modification of the fiber surfaces. It also reviews recent studies focused on the use of nonwoven fabric devices in membrane chromatography and provides some perspectives on the challenges that need to be overcome to increase binding capacities, decrease residence times, and reduce pressure drops so that eventually they can replace resin column chromatography in downstream process operations.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%