Computational Guided Method Applied to LSPR‐Based Biosensor for Specific Detection of the Four‐Serotypes of Dengue Virus in Seropositive Patients

Abstract

In this paper, a localized surface plasmon resonance (LSPR)‐based biosensor, computationally guided to detect selectively the four Dengue virus (DENVx) serotypes in seropositive patients, is presented. The behavior of gold nanoparticles in the shape of nanorods is theoretically and numerically studied as a function of induced structural variations, which are experimentally evidenced due to the bio‐interaction between the target analytes and its surface during the detection process. Additionally, with the implementation of the largest Lyapunov exponent, it is possible to calculate the notion of predictability for the experimental results, observing chaotic systems with a very low probability of repetition. Due to the above, when analyzing the recurrence map associated with the obtained resonance curve generated by the LSPR system, the genetic similarity of DENV3/DENV2 and DENV4/DENV1 is evidenced. Finally, the biosensors are validated by analyzing samples of seronegative patients for DENVx and seropositive ones for other Flaviviruses such as Zika virus, Yellow Fever virus, and Saint Louis Encephalitis virus.

Insights into Gold Nanoparticles Possibilities for Diagnosis and Treatment of the Head and Neck Upper Aerodigestive Tract Cancers

“…At the nanometer scale, their sensitivity is enhanced exponentially, and any minimal alteration can be detected and measured via dynamic light scattering, a property known as plasmonic shifts. Through a conventional plate spectrophotometry reader coupled with ultraviolet–visible light (UV-Vis), it is possible to perform diagnostics using AuNPs LSPR shifts [ 8 , 17 ], as well as via other spectroscopic techniques and tools; most of these spectrophotometers are already available for purchase. Another feature worth mentioning is the colorimetric changes, observable to the naked eye, that generate spectroscopic signatures that work as fingerprints, allowing their use for diagnosis and therapy.…”

Challenges and perspectives of multi-virus biosensing techniques: A review

“…LSPR technology can also be used for viral gene analysis, as shown in a recent study that detected four dengue viruses with high sensitivity and selectivity [ 25 ]. This biosensor allowed us to investigate the genetic similarity between viruses by analyzing the recurrence relation matrix.…”

“…The precise design of transducers, state-of-the-art nanofabrication, and bio-interface innovation have improved the sensitivity, accuracy, and selectivity of multi-virus biosensors. In addition, the amount of sample for detection has been minimized from the picoliter to the microliter scale, while advancements in artificial intelligence make the prediction of viral species possible [ 25 ].…”